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Articles by Y.M. Abdel-Hadi
Total Records ( 2 ) for Y.M. Abdel-Hadi
  M.S. Khairul Afizi , B.S. Siti Fatimah , N.S. Mariana and Y.M. Abdel-Hadi
  Motile Aeromonad septicemia is the main devastating disease in cultured fish and caused mainly by Aeromonas hydrophila. Development of pathogenic bacterial resistance caused by the excessive use of chemotherapeutics is the main disadvantage of antibiotic application. Thus, the sensitivity of certain commercial antibiotics and common herbs was evaluated against pathogenic A. hydrophila, A. sobria and A. caviae, isolated from Malaysian and Egyptian cultured fish, mainly tilapia. A suspension of freshly cultured isolates was prepared and spread over the Muller’s Hinton agar plates to study their sensitivity to both antibiotics and herbs. Double-fold dilution was used to determine the Minimal Inhibitory Concentration (MIC) for the effective herbs at 100, 50, 25, 12.5 and 6.25%. Results revealed high resistance of the 6 tested Aeromonas isolates against most of the screened antibiotics except Ciprofloxacin and Gentamicin. Regarding herbal sensitivity, only Origanum vulgare showed an efficacy and inhibition zone against all isolates except A. hydrophila isolated from cultured tilapia in Egypt which exhibited resistance to that herb. The MIC ranged from 25-55% for the Egyptian isolates (25% for A. caviae and 55% for A. sobria) and 24-30% for the Malaysian isolates (24% for A. hydrophila, 25% for A. caviae and 30% for A. sobria). This study recommends the mandate of effective vaccine development against A. hydrophila to protect cultured fish from that pathogen due to its high resistance for both herbs and antibiotics.
  Y.M. Abdel-Hadi , Mariana Nor Shamsudin , K. Yusoff and Shater Zakaria
  Monovalent, killed and live attenuated vaccines of Aeromonas hydrophila and Pseudomonas putida were used in the immunization of red tilapia against Motile Aeromonad and Pseudomonad septicemias. There were 4 treatments and a 5th control group with 3 replicates per each. A 4th replicate was kept for replacement of natural mortality among the experimented fish. The 4 treatments included, Heat-killed vaccine of A. hydrophila, Live-attenuated vaccine of A. hydrophila (using herbs), Heat-killed vaccine of P. putida and Live-attenuated vaccine of P. putida. A total of 160 brood stocks of O. niloticus with 250 g average body weight were used for all treatments (8 fish per each glass aquarium). Vaccination was conducted via the Intra Peritoneal route (I/P) as an initial dose followed by 2 booster doses every 2 weeks. The last dose was applied via the immersion route. The evaluation of vaccination was carried out through periodical antibody titration of the serum of the examined fish (every 2 weeks) using direct agglutination method as well as by the experimental challenge 3 months after the initial immunization. Results revealed that there were a significant difference between the vaccinated and non vaccinated fish of the control group regarding antibody titers and Relative Percent Survival (RPS) of the challenge test. Differences in immunity levels within the vaccinated groups themselves were demonstrated.
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