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by
Y.L. Li |
Total Records (
3 ) for
Y.L. Li |
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Q. Huang
,
Y.L. Li
,
X. Xu
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Y. Huang
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Z.W. Cui
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D.Y. Yu
and
W.F. Li
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The aim of this study is to investigate the effects of Bacillus subtilis BS02 on viability and immune responses of RAW 264.7 cells. RAW 264.7 cells were stimulated with and without B. subtilis spores for 12 h in the treatment group and the control group, respectively. Cytotoxic effect of B. subtilis BS02 on macrophages was measured by cell viability and LDH cytotoxicity assay. Immune responses of macrophage elicited by B. subtilis BS02 were analyzed by measuring the activities of Acid Phosphatase (ACP) and Lactate Dehydrogenase (LDH), the production of Nitric Oxide (NO) and inducible Nitric Oxide Synthase (iNOS) and the secretion of inflammatory cytokines. The results showed that after 12 h incubation, B. subtilis BS02 spores had no influence on viability of RAW 264.7 cells; ACP and LDH activities, the production of NO and iNOS, the levels of inflammatory cytokines [Tumor Necrosis Factor-alpha (TNF-α), Interferon-gamma (IFN-γ), Interleukin-1 beta (IL-1β), IL-6, IL-8, IL-10 and IL-12] were significantly higher than that in the control group (p<0.01). These results indicate that B. subtilis BS02 is not only safe for RAW 264.7 cells but also can activate macrophage immune function. |
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W.F. Li
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Z.W. Cui
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I.R. Rajput
,
Y.L. Li
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H.Z. Wu
and
D.Y. Yu
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The free radical scavenging systems remove most peroxide which shows antioxidantion capacity of body and lactic acid producing bacteria have capacity to support the body in the mechanism. The present study was initiated to investigate the antioxidantion functioning property of Enterococcus faecium 1 (EF1) to Caco-2 cells under oxidative stress condition. The cells were cultured and randomly divided into 4 groups, the control group (T0), the oxidative stress group (T1), Tert-Butyl Hydroquinone (TBHQ) with addition of H2O2 (T2) and EF1 with combination of H2O2 (T3). The results showed that Total Antioxidation Capacity (T-AOC), Catalase (CAT), Superoxide Dismutase (SOD) activities, Glutathione (GSH) contents in the cultured supernatant and SOD activity of the cells lysate at 12 h increased (p<0.05) in T3 as compared to T1. The supernatant of cells cultured at 12 h significantly improved the SOD, GSH-Px activities and GSH contents in T3. While, Anti Superoxide Anion Free Radical (ASAFR), CAT, SOD and Glutathione Peroxidase (GSH-Px) activities (p<0.05) increased in supernatant at 48 h, conversely Malondialdehyde (MDA) contents was significantly reduced (p<0.05). The SOD activity and GSH contents of cells lysates at 48 h showed similarly reduced (p<0.05). The comparative findings of T3 to T2 supernatant and lysate of cells at 48 h showed significant increase in T-AOC, CAT, SOD, GSH-Px activities and GSH contents of supernatant and in lysate POD activity and GSH contents significantly increased. While, decline (p<0.05) was observed in the MDA contents in supernatant and lysates of T3. The findings revealed that Enterococcus faecium 1 could increase the antioxidation functioning activity of Caco-2 cells under oxidative stress condition. |
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X.L. Li
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X.M. Zou
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P. Gao
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Y.L. Li
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H. Wang
and
X.W. Chen
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Objective: This study was designed to evaluate the role of nitric oxide (NO) in ischemia-reperfusion injury (IRI) and acute rejection (AR) in rat intestinal transplantation, using administration of the NO inhibitor NG-nitro-L-arginine methyl ester (L-NAME). Materials and Methods: Rats that underwent orthotopic allogeneic intestinal transplantation were assigned to 2 groups. In the normal allograft group (Wistar to Sprague-Dawley rats), L-NAME 0 mg/kg/d (group 1-1), 4 mg/kg/d (group 1-2), 8 mg/kg/d (group 1-3), or 12 mg/kg/d (group 1-4) was injected intraperitoneally. In the high responder allograft group (Dark Agouti to Lewis rats), L-NAME 0 mg/kg/d (group 2-1) or 8 mg/kg/d (group 2-2) was injected intraperitoneally. Survival times were observed and maltose absorption tests performed as well as light microscopic examination of the grafts.Results: The mean survival time of group 1-3 was significantly prolonged compared with group 1-1 (P < .01). In group 2, the survival time of group 2-2 was significantly prolonged compared with group 2-1 (P < .01). Histological changes showed IRI was attenuated in group 1–2 compared with group 1-1, whereas it was aggravated in groups 1-3 and 1-4. Treatment with L-NAME (8 mg/kg/d) attenuated the graft damage of AR in groups 1 and 2. Maltose absorption tests showed that inhibition of NO impaired maltose absorption.Conclusion: This study suggested that NO plays a dual role as both a cytotoxic and a cytoprotective factor in IRI, and may serve as a kind of cytotoxic medium in AR in rat intestinal allotransplantation. |
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