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Articles by Y.A. Elhassaneen
Total Records ( 5 ) for Y.A. Elhassaneen
  Y.A. Elhassaneen and M.I. Sanad
  Selenium, vitamin C, pungency, amino acids, phenolics content and antioxidant activities of two Egyptian onion varieties, namely white (Giza-6) and red (Beheri) onions have been studied. Data analysis showed that the red variety presents higher values for selenium, vitamin C and sulphur-containing amino acids. Concerning pungency, white onion can be classified as intermediate pungency (8.24 μmol of Pyruvic acid/100 g fresh wt.) and red as pungent (11.37 μmol of pyruvic acid/100 g fresh wt.). The phenolic acids, flavonols, anthocyanins and total phenolics content in red variety (81.59, 70.38, 7.56 and 187.17 mg/100 g fresh wt., respectively) were higher than for white variety (72.47, 32.49, 4.90 and 131.65 mg/100 g fresh wt., respectively). Consequently, antioxidant activity was higher for the red variety. Correlation analysis indicates that phenolic compounds beside other factors including Se and sulphur-containing amino acid contents play the major role in the antioxidant activity of onion bulbs. The antioxidant capacity of freeze dried powder from both onion varieties was also tested in sunflower oil-in-water emulsions and hydroperoxide formation was monitored during storage at 40°C. In accordance with differences in Se, sulphur-containing amino acid and phenolics content, Egyptian red onions had better antioxidant activity, while white onions was only effective in the early stages of the oxidation process. These data indicates that red variety has higher potential health benefits related to the presence of antioxidant compounds.
  S.E. Hassab El-Nabi and Y.A. Elhassaneen
  Simple, sensitive and rapid techniques were modified for detection of total genomic damage of DNA (TGD-DNA), Electrophoretic pattern of RNA and molecular apoptosis. The techniques were applied in both cell culture and solid tissues. In cell culture, the cells were lysated directly in the gel wells and mixed with loading buffer. In solid tissues, 5-10 mg were squeezed and lysated in ependorf tubes, then 1 mg of lysated tissues was loaded in the gel wells and mixed with loading buffer. This technique elaborated the DNA damage as smear or apoptotic bands. Detection of molecular apoptosis by gel electrophoresis was modified in our laboratory, where, salt out extraction method of DNA was applied using small amount of tissues (5-10 mg), squeezed by blue tips and lysated quickly by lysing buffer. Mixture of loading mix was developed which has a great effect on the stability and resolution of apoptotic bands. The developed technique is very simple, fast and economic to prepare DNA apoptotic ladder from liver, spleen and human leukocytes. Induction of apoptosis was done chemically by methotraxate in liver, spleen and thymus of rats, or naturally by postmortem changes in liver of rat and storage blood of human. Some factors were studied for stability and resolution of apoptotic ladder such concentrations of SDS in lysing buffer, effects of temperatures, glycerol and our loading mix. Concentration of SDS has no effect on the intensity of apoptotic bands in extracted DNA, while in cell or tissue lysate, the apoptotic bands appeared sharp at low concentrations of SDS (0.5-1%). After preparation of DNA and addition of TE buffer the best temperature that keeps on the stability of DNA was 37 °C. Our loading mix and glycerol protects apoptotic bands from degradation at room temperature or -20 °C. The stability of home made apoptotic ladder was stable for 6 month or more at room temperature.
  Y.A. Elhassaneen , S.A. Saleh , S.F. El-Abd , M.M. El-Sayed and N.N. El-Nashar
  The aim of this study was to investigate the protective role of Cinnamon Flavonoid Extract (CFE) against histopathological changes in albino rats of Wistar strain treated with Dibutylnitrosamine (DBNA) for 12 weeks. The results indicated that rats treated with DBNA recorded decreasing in the total body and liver weights and increasing in spleen and kidney weights with significant values when compared with the control group all over the experiment period 4, 8 and 12 weeks. Addition of CFE by 150 and 300 mg kg-1 b.wt./day in the presence of nitrosamine induced significant improvements in all organs weights. Also, DBNA treated group had histopathological changes on liver through degeneration hyperemia, inflammatory reaction, kidney through hemorrhages renal casts hyperemia, inflammatory reaction and also urinary bladder through papillary hyperplasia with papillary projection formation in the cell layer of the lining epithelium. The co-treatment of CFE with DBNA leads to prevent some of the previous histopathological changes mainly on liver and urinary bladder and secondary on the kidney. It could be concluded that CFE was effective in protecting against DBNA-induced histopathological changes. These results supported present hypothesis that CFE contains several compounds that are able to prevent or inhibit DBNA toxicity.
  Y.A. Elhassaneen , H.A. El-Fadaly and N.E. Dewan
  In the present study a trial was carried out to develop a new biotechnique for removal some toxic substances from edible oils as affected by deep-fat frying operation. Wastewater samples from Oil and Soap Company was used as a source of oil using bacteria and very simple technique for treatment the deep-fat frying oils with bacterial isolates was applied. A decrease in many toxic and/or carcinogenic compounds was observed in treated oils included free fatty acids (FFA), peroxide value (PV), malonaldehyde (MDA) and benzo(a)pyrene B(a)P contents by different rates. The rates of decreasing were increased with the increasing of oil concentration in cultural medium of used bacteria up to 15%. Spectroscopic analysis showed that many absorbance peaks 3420, 3120, 1750 and 970-1000 nm have been disappeared which means the removal of many corresponding toxic compounds included hydroperoxides, polymerization products, carbonyl groups or acids and trans-ethylenic double bonds of fatty acids as the result of treatment by Bacillus firmus. Results suggested that this biotechnique could be adequately coordinated with the commercial concern and the interests of society or the administration, which aim to improve consumer safety.
  Y.A. Elhassaneen and A.A. Abd El-Moaty
  It has long been known that frequent oral exposure to insect- infested flour run an increased risk of many diseases, including cancer, kidney failure etc in experimental animals. The susceptibility to all of these diseases may be a consequence of superimposed oxidative stress due to lack of defensive mechanisms. In concern with this hypothesis, the present study was carried out to assess the influence of feeding insect-infested wheat flour on the oxidant and antioxidant status in rats. Wheat flour samples were infested with Tribolium confusum for eight weeks then introduced immediately in feeding the rats for ten weeks. During the feeding period, blood samples were drawl for hematological studies periodically after each two weeks. The obtained data revealed that activities of antioxidant enzymes GSH-Px and GSH-R were found lower in the erythrocytes of rats feeding insect-infest wheat flour. By ten weeks of feeding the infested flour, the lowering ratios in these enzyme activities were 9.27 and 32.09% respectively. The GSH/GSSG ratio in plasma, which provide a sensitive index of whole body oxidative stress, were lower significantly and recorded 5.29 ±1.35. Plasma levels of antioxidant vitamin (vitamins A, C and E) were also significantly lower in plasma of rats feeding insect-infested wheat flour for ten weeks of feeding the infested flour and the lowering ratios for these vitamins level were 38.25, 37.49 and 43.21%, respectively. Additionally, levels of oxidants in plasma, TBARS, nitrite (NO2) and nitrate (NO3), were higher by the ratio of 124.92, 188.97 and 178.35%, respectively. Results suggested that enzymatic antioxidant defence system of erythrocytes was depressed and the erythrocytes were exposed to oxidant stress due to insect-infested flour feeding. Increased plasma TBARS, NO2 and NO3 levels indicated that not only erythrocytes but also some other tissues and cells might be exposed to the radical stress by insect-infested flour. Feeding insect-infested flour also caused significant changes in the levels of antioxidant vitamins partly protected erythrocytes against such harmful effects of feeding on infested flour by scavenging free radical species and by activating or inducing antioxidant enzymes.
 
 
 
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