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Articles by Y. Ono
Total Records ( 3 ) for Y. Ono
  M. A. I. Khan , K. Ueno , S. Horimoto , F. Komai , K. Tanaka and Y. Ono
  This study evaluated the upland weed control potentiality, germination inhibition ability and growth suppression efficiency of the five combinations of green tea waste – rice bran compost (GRC). GRC was prepared by mixing green tea waste and rice bran at five ratios, and allowed to decompose for 5 mo. Application of GRC suppressed weed growth up to 93.4% in number and 95.4% in dry weight in 2004, and 80.7% in number and 73.4% in dry weight of weeds in 2005, as compared with the untreated control (only soil) under the greenhouse condition. Among the five combinations of GRC, rice bran alone (RC) showed the significantly highest and green tea waste alone (GC) showed the lowest weed suppressing activity in both years. The weed control potentiality of GRC was increased by the increase of rice bran percentages in the mixture. The exudates of GRC inhibited the hypocotyl and radicle elongation of lettuce seedlings when examined by the sandwich method. The water extracts of GRC also inhibited the germination and radicle elongation of the test species in the seed germination tests. The growth inhibitory activity of RC was greater than that of GC, and radicle elongation was more sensitive than seed germination and hypocotyl elongation in all bioassays. The inhibitory activity of GRC water extract varied with the test species and was higher for the dicotyledonous species than monocotyledonous species. The inhibitory effect on seed germination and seedling growth increased as the extract concentration increased. The concentration dependent responses of test species to the water extract of GRC indicated that it might contain phytotoxic substances that were responsible for growth inhibition. Moreover, GRC promoted spinach growth significantly compared with the untreated control. These results suggest that the use of 30% green tea waste + 70% rice bran mixture compost (GRC-3) might be useful to control the upland weeds and enhance spinach growth among the five combinations of GRC, and it may reduce the use of hazardous synthetic agrochemicals. Optimization of the combination of composting materials is necessary for the multipurpose use compost.
  M. A. I. Khan , K. Ueno , S. Horimoto , F. Komai , K. Tanaka and Y. Ono
  Rice bran compost (RC) was evaluated under greenhouse and field conditions for eco-friendly weed control of vegetable crops in organic farming systems. RC was applied 7 days before spinach seed sowing, at the rate of 0% (control), 10%, 20% and 30% of soil (v/v) in the greenhouse and 2 kg m-2 under field conditions. RC significantly controlled the number and dry weight of weeds, and the weed control potentiality was increased by the increased application of RC in both years. The efficacy of RC in reducing weed emergence and dry weight of broadleaf species was higher than grass weeds. Among the broad leaf weeds, Lamium amplexicaule L was the most sensitive to RC and was drastically reduced by the increased application of RC. Under field conditions, the total number of weeds and the dry weight of weeds were also significantly reduced with the application of RC, compared with the untreated control. The growth inhibition potentiality of water soluble fractions of RC was also tested with seed germination and radicle elongation of test species (barley, oat, tomato and lettuce). The concentration response curves for the test species were drawn by plotting the percentage of germination against the logarithm of the concentration. Considering the I25 value (defined as 25% inhibition of control) both for seed germination and radicle elongation, it was indicated that water soluble fractions of RC was more effective in the suppression of dicotyledonous species (tomato and lettuce) than monocotyledonous species (barley and oat). The results of the preliminary experiment with fresh rice bran under greenhouse conditions showed that fresh bran suppressed spinach seed germination when applied as 10% rice bran in soil but RC did not show a significantly negative effect up to 20% RC application, compared with the control. Moreover, RC enhanced spinach growth. Considering both the weed control potentiality and spinach growth enhancing ability of RC, the effective appplication rate of RC is between 10-20% of soil (v/v) for weed control as well as the successful growth of spinach. This research indicates that RC has the potential of being used as a natural herbicides.
  Y. Ono , Y. Ito , K. Kaneko , Y. Shibata-Watanabe , T. Tainaka , W. Sumida , T. Nakamura , H. Kamei , T. Kiuchi , H. Ando and H. Kimura
  Cytomegalovirus (CMV), Epstein-Barr virus (EBV), and human herpesvirus-6 (HHV-6) cause symptomatic diseases in liver transplant recipients. The loads of these viruses, the associations between viral DNAemia, serologic status, and acute rejection reactions were investigated in a group of 17 juvenile and 17 adult recipients of living donor liver transplantation (LDLT) for a median of 8 weeks posttransplantation. At least 1 plasma sample from 15/34 (44.1%) patients was positive for CMV DNA. For most of the CMV-positive patients, the CMV DNA appeared in the second week of LDLT, and disappeared by the eighth week. A minimum of 200 EBV DNA copies/μg peripheral blood mononuclear cell DNA (defined as positive for EBV) was detected in 5/34 (14.7%) patients, and the number of EBV-positive children was significantly greater than the number of EBV-positive adults. In most of the EBV-positive patients, the EBV loads increased after 4 weeks posttransplantation. Plasma HHV-6 was detected in 7/34 (20.6%) patients. HHV-6 DNA appeared for a short period from the second week of LDLT. In addition, 8 of the 19 virus-positive recipients carried 2 viruses, with the combination of CMV and HHV-6 being the most frequent. Serologic status seemed to be an important factor for all 3 viral infections. The rate of acute cellular rejection was not significantly higher in the CMV-, EBV-, or HHV-6-positive groups. Simultaneous monitoring for 3 herpesviruses revealed the impact of these viruses on LDLT recipients.
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