Asian Science Citation Index is committed to provide an authoritative, trusted and significant information by the coverage of the most important and influential journals to meet the needs of the global scientific community.  
ASCI Database
308-Lasani Town,
Sargodha Road,
Faisalabad, Pakistan
Fax: +92-41-8815544
Contact Via Web
Suggest a Journal
 
Articles by Y. Li
Total Records ( 15 ) for Y. Li
  H Liu , S Li , Y Zhang , Y Yan and Y. Li
 

Glutamate decarboxylase 65 (GAD65) produces -aminobutyric acid, the main inhibitory neurotransmitter in adult mammalian brain. Previous experiments, performed in brain, showed that GAD65 gene possesses two TATA-less promoters, although the significance is unknown. Here, by rapid amplification of cDNA ends method, two distinct GAD65 mRNA isoforms transcribed from two independent clusters of transcription start sites were identified in post-natal rat testis. RT–PCR results revealed that the two mRNA isoforms had distinct expression patterns during post-natal testis maturation, suggesting that GAD65 gene expression was regulated by alternative promoters at the transcription level. By using GAD65-specific antibodies, western blotting analysis showed that the 58-kDa GAD65, N-terminal 69 amino acids truncated form of full-length GAD65 protein, was developmentally expressed during post-natal testis maturation, suggesting that GAD65 gene expression in testis may also be regulated by post-translational processing. Confocal immunofluorescence microscopy revealed that GAD65 protein was presented in Leydig cells of Day 1 testis, primary spermatocytes and spermatids of post-natal of Day 90 testis. The above results suggested that GAD65 gene expression is dynamically regulated at multiple levels during post-natal testis maturation.

  A.M. Fouad , Y. Li , W. Chen , D. Ruan , S. Wang , W. Xie , Y.C. Lin and C.T. Zheng
  Objective: Manganese (Mn) is a crucial trace element for poultry nutrition because it has multiple physiological functions. Thus, the main goal of this study was to evaluate the effects of dietary Mn supplementation on laying performance, egg quality and antioxidant status in Shanma laying ducks. Methodology: Five hundred and four Shanma laying ducks, at 17 weeks of age, were randomly assigned to 7 treatments, with 6 replicates per treatment and 12 ducks per replicate. Birds were fed the same basal diet, which was supplemented with 0.0 (control), 15, 30, 45, 60, 75 or 90 mg Mn/kg in the form of Mn-sulfate. Results: Results showed that dietary Mn supplementation did not affect egg production, egg weight, feed conversion ratio, egg mass, egg quality, tibia characteristics, total antioxidant capacity, copper-zinc superoxide dismutase or lipid peroxidation (malondialdehyde), but supplementing 90 mg Mn/kg diet significantly (p<0.05) improved the activities of total superoxide dismutase, Mn-containing superoxide dismutase and increased Mn content in egg yolk compared with the control group. Conclusion: These results indicate that corn-soybean meal diet containing sufficient amount of Mn for laying performance and egg quality in Shanma laying ducks under the conditions of current experiment, but adding 90 mg Mn/kg basal diet is required to improve the activities of Mn-containing superoxide dismutase and total superoxide dismutase and elevate Mn content in their egg yolk.
  T Du , M. R Lewin , H Wang , X Ji , H. H Bohn , T Xu , L Xu , Y Zhang and Y. Li
  Objective

To investigate the circadian and seasonal patterns in the presentation of acute upper gastrointestinal bleeding (AUGIB) in Beijing, China.

Methods

Medical records of the Beijing Emergency Medical Service System (EMSS) for 1 August 2005 to 31 July 2007 were reviewed; all patients diagnosed with AUGIB were included in the study.

Results

2580 patients were recorded in the EMSS system with a diagnosis of AUGIB during the study period. 1888 (73%) were male and 692 (27%) were female. Mean age was 53±20 years for male patients and 63±21 years for female patients. Significant differences in the presentation of AUGIB were noticed between seasons (p<0.001) and months (p<0.001). The number of cases in cold months (from December to April) was significantly higher than that in warm months (June to September). There was a significant circadian rhythm; there were fewer cases during daytime hours compared with night-time hours (p<0.001).

Conclusions

The presentation of AUGIB in Beijing has a clear seasonal and circadian rhythm. Circadian and seasonal rhythms associated with AUGIB may aid in identifying modifiable risk factors in individuals and populations.

  Y. Li , M. Dong , X. Xiang , Z. Xiang and Y. Pang
  In this study, we established an optimization control model and the corresponding computer algorithm to estimate the diffusion coefficient of the drug releasing in the spherical device. First, based on the diffusion equation in the spherical device in the polar coordinates system, the optimal control model was given to compute the diffusion coefficient in the drug releasing problem in the sphere device. Next, the Least Square Method based on the Separation Variables (LSMSV) was used to solve the problem to estimate the appropriate diffusion coefficient. Finally, a numerical example was presented to show that the control model and the numerical method are valid for computing the diffusion coefficient of the drug releasing in the sphere device.
  Q.R. Hou , J.Y. Wang , H.H. Wang , Y. Li , G.X. Zhang , Y. Wei and Hassan
  Lysozyme plays important role in vertebrates and invertebrates for its unique protein structure and enzyme activity. In the present study, the potential physiological function of the chicken lysozyme gene (LYZ) was studied by SNP. 329 J+ line Jinghai Yellow chicken were involved in this experiment. To determine gene mutations, DNA analysis of the LYZ gene, including Single Strand Confirmation Polymorphism (SSCP) and direct DNA sequencing, was performed. Chicken Body Weight (BW) were analyzed by GLM of SAS software. DNA sequencing demonstrated a G111A transition of exon 1, a T1426C transition and a C1492T transition of exon 2, all of them were silent mutations that caused no alteration in amino acid sequence. Statistical analysis indicated that, AA and CC genotype related with high BW, TT and CN genotype contributed to low BW. Therefore, results suggested that there was a possibility of the LYZ gene SNPs acting as a genetic marker for growth traits of Jinghai Yellow chicken.
  J.J. Xu , S.S. Wang , D.D. Liu , L.Q. Cao , Y. Li and J.P. Tao
  Coccidiosis causes diarrhoea, dehydration and death in geese. Eastern China is a large goose-raising area in China but the coccidial infection status in geese in this region has not been reported as so far. To understand Coccidia species and infection rate, fecal samples were collected from 146 randomly selected clinically healthy domestic goose populations between August 2010 and July 2011. Oocysts were separated by a floatation technique using saturated saline. Coccidia species was identified by examining morphological features of the sporulated oocysts and further verified through animal regression test. The results showed that coccidian oocysts were detected in 87.67% of the goose population. Eight different species of the Eimeriidae family were identified, namely T. parvula Koltan (90.63%), E. hermani Farr (76.56%), E. stigmosa Klimes (48.44%), E. nocens Koltan (35.94%), E. fulva Farr (15.63%), E. anseris Koltan (9.38%), E. farri Hanson, Levine and Ivens (4.69%) and I. anseris Koltan (4.69%). Among them, the first three species were most prevalent. In addition, 87.50% of the farms had at least two commensal Coccidia species, indicating concurrent infection existed widely in geese. The analysis of coccidial infection with age revealed that the infection mainly occurred in geese older than 30 days and the infection rate increased with ages. In summary, the results suggest that the coccidial infection was common in domestic geese in eastern China and measures for prevention and treatment of coccidiosis were needed for this area.
  T. Detchprohm , M. Zhu , Y. Li , Y. Xia , L. Liu , D. Hanser and C. Wetzel
 

We demonstrate homoepitaxial growth of GaInN/GaN-based green (500–560 nm) light emitting diodes (LEDs) on a-plane and m-plane quasi-bulk GaN prepared by hydride vapor phase epitaxy (HVPE). We find that in order to achieve an emission peak wavelength beyond 500 nm, a minimum InN-fraction of 14% is needed for both, a- and m-plane quantum wells (QWs), while 8% are enough for c-plane-oriented QWs. Besides increasing the InN-fraction in these non-polar QWs, widening the QW also proves to effectively shift the emission to longer wavelengths without loosing efficiency with the benefit of maintaining a low InN-fraction.

  P Gao , K Liu , L Liu , Z Wang , Z Liao , Z Xu , W Wang , X Bai , E Wang and Y. Li
 

The higher-order harmonic resonances, including second and third harmonic modes, were induced by applying alternative current signals inside a high-resolution transmission electron microscope (HRTEM), which have been used to study the mechanical properties of individual cadmium sulphide (CdS) nanowires. Young's moduli (E) and mechanical quality factors (Q) of individual CdS nanowires with diameters in the range of 50–350 nm were measured with the assistance of the mechanical resonances. The results indicate that the smooth nanowires have larger E and Q in comparison with the rough nanowires, and for the rough nanowires, E and Q increase with increasing diameters. The morphology- and size-dependent mechanical properties of CdS nanowires are directly correlated with their structure, as imaged by in situ TEM.

  M Jiang , Y Ma , C Chen , X Fu , S Yang , X Li , G Yu , Y Mao , Y Xie and Y. Li
 

Androgen signaling plays an important role in many biological processes. Androgen Responsive Gene Database (ARGDB) is devoted to providing integrated knowledge on androgen-controlled genes. Gene records were collected on the basis of PubMed literature collections. More than 6000 abstracts and 950 original publications were manually screened, leading to 1785 human genes, 993 mouse genes, and 583 rat genes finally included in the database. All the collected genes were experimentally proved to be regulated by androgen at the expression level or to contain androgen-responsive regions. For each gene important details of the androgen regulation experiments were collected from references, such as expression change, androgen-responsive sequence, response time, tissue/cell type, experimental method, ligand identity, and androgen amount, which will facilitate further evaluation by researchers. Furthermore, the database was integrated with multiple annotation resources, including National Center for Biotechnology Information, Gene Ontology, and Kyoto Encyclopedia of Genes and Genomes pathway, to reveal the biological characteristics and significance of androgen-regulated genes. The ARGDB web site is mainly composed of the Browse, Search, Element Scan, and Submission modules. It is user friendly and freely accessible at http://argdb.fudan.edu.cn. Preliminary analysis of the collected data was performed. Many disease pathways, such as prostate carcinogenesis, were found to be enriched in androgen-regulated genes. The discovered androgen-response motifs were similar to those in previous reports. The analysis results are displayed in the web site. In conclusion, ARGDB provides a unified gateway to storage, retrieval, and update of information on androgen-regulated genes.

  L Jin , D Martynowski , S Zheng , T Wada , W Xie and Y. Li
 

The retinoic acid-related orphan receptor (ROR) has important roles in development and metabolic homeostasis. Although the biological functions of ROR have been studied extensively, no ligands for ROR have been identified, and no structure of ROR has been reported. In this study, we showed that hydroxycholesterols promote the recruitment of coactivators by ROR using biochemical assays. We also report the crystal structures of the ROR ligand-binding domain bound with hydroxycholesterols. The structures reveal the binding modes of various hydroxycholesterols in the ROR pocket, with the receptors all adopting the canonical active conformation. Mutations that disrupt the binding of hydroxycholesterols abolish the constitutive activity of ROR. Our observations suggest an important role for the endogenous hydroxycholesterols in modulating ROR-dependent biological processes.

  A.M. Fouad , Y. Li , W. Chen , D. Ruan , S. Wang , W.G. Xie , Y.C. Lin and C.T. Zheng
  This study was designed to investigate the influences of dietary copper (Cu) on laying performance, egg quality, plasma total cholesterol (TC), high-density lipoprotein cholesterol (HDLC) and low density lipoprotein-cholesterol (LDLC) concentrations in Shanma laying ducks. A total of 504 egg laying ducks aged 17 week were randomly allotted to seven groups (twelve ducks per replicate and six replicates each treatment). The control group was fed a corn-soybean meal diet, whereas the treatment groups were fed corn-soybean meal diets supplemented with 4, 8, 12, 16, 20, or 24 mg Cu-sulfate/kg. Egg production, egg weight, egg mass, feed conversion ratio, broken egg rate, abnormal egg rate, Haugh unit, yolk color, albumen height, eggshell weight, eggshell percent, eggshell breaking strength, eggshell thickness, plasma TC, HDLC and LDLC concentrations did not appear response to dietary Cu levels. Our results indicated that corn-soybean meal diet containing sufficient amount of Cu for laying performance and egg quality in laying ducks under the conditions of current experiment. Also, laying performance, plasma TC, HDLC, LDLC concentrations and egg quality were not sensitive traits to low levels of Cu.
  Z Huang , Y Shi , B Cai , L Wang , Y Wu , B Ying , L Qin , C Hu and Y. Li
 

Objectives. To discover novel potential biomarkers and establish a diagnostic pattern for SLE by using proteomic technology.

Methods. Serum proteomic spectra were generated by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) combined with weak cationic exchange magnetic beads. A training set of spectra, derived from analysing sera from 32 patients with SLE, 43 patients with other autoimmune diseases and 43 age- and sex-matched healthy volunteers, was used to train and develop a decision tree model with a machine learning algorithm called decision boosting. A blinded testing set, including 32 patients with SLE, 42 patients with other autoimmune diseases and 40 healthy people, was used to determine the accuracy of the model.

Results. The diagnostic pattern with a panel of four potential protein biomarkers of mass-to-charge (m/z) ratio 4070.09, 7770.45, 28 045.1 and 3376.02 could accurately recognize 25 of 32 patients with SLE, 36 of 42 patients with other autoimmune diseases and 36 of 40 healthy people.

Conclusions. The preliminary data suggested a potential application of MALDI-TOF MS combined with magnetic beads as an effective technology to profile serum proteome, and with pattern analysis, a diagnostic model comprising four potential biomarkers was indicated to differentiate individuals with SLE from RA, SS, SSc and healthy controls rapidly and precisely.

  F Yokoi , G Yang , J Li , M. P DeAndrade , T Zhou and Y. Li
 

DYT1 early-onset generalized torsion dystonia is an inherited movement disorder caused by mutations in DYT1 coding for torsinA with ~30% penetrance. Most of the DYT1 dystonia patients exhibit symptoms during childhood and adolescence. On the other hand, DYT1 mutation carriers without symptoms during these periods mostly do not exhibit symptoms later in their life. Little is known about what controls the timing of the onset, a critical issue for DYT1 mutation carriers. DYT11 myoclonus-dystonia is caused by mutations in SGCE coding for -sarcoglycan. Two dystonia patients from a single family with double mutations in DYT1 and SGCE exhibited more severe symptoms. A recent study suggested that torsinA contributes to the quality control of -sarcoglycan. Here, we derived mice carrying mutations in both Dyt1 and Sgce and found that these double mutant mice showed earlier onset of motor deficits in beam-walking test. A novel monoclonal antibody against mouse -sarcoglycan was developed by using Sgce knock-out mice to avoid the immune tolerance. Western blot analysis suggested that functional deficits of torsinA and -sarcoglycan may independently cause motor deficits. Examining additional mutations in other dystonia genes may be beneficial to predict the onset in DYT1 mutation carriers.

  A Dessein , C Chevillard , V Arnaud , X Hou , A. A Hamdoun , H Dessein , H He , S. A Abdelmaboud , X Luo , J Li , A Varoquaux , A Mergani , M Abdelwahed , J Zhou , A Monis , M. G.R Pitta , N Gasmelseed , S Cabantous , Y Zhao , A Prata , C Brandt , N. E Elwali , L Argiro and Y. Li
 

Abnormal fibrosis occurs during chronic hepatic inflammations and is the principal cause of death in hepatitis C virus and schistosome infections. Hepatic fibrosis (HF) may develop either slowly or rapidly in schistosome-infected subjects. This depends, in part, on a major genetic control exerted by genes of chromosome 6q23. A gene (connective tissue growth factor [CTGF]) is located in that region that encodes a strongly fibrogenic molecule. We show that the single nucleotide polymorphism (SNP) rs9402373 that lies close to CTGF is associated with severe HF (P = 2 x 10–6; odds ratio [OR] = 2.01; confidence interval of OR [CI] = 1.51–2.7) in two Chinese samples, in Sudanese, and in Brazilians infected with either Schistosoma japonicum or S. mansoni. Furthermore, SNP rs12526196, also located close to CTGF, is independently associated with severe fibrosis (P = 6 x 10–4; OR = 1.94; CI = 1.32–2.82) in the Chinese and Sudanese subjects. Both variants affect nuclear factor binding and may alter gene transcription or transcript stability. The identified variants may be valuable markers for the prediction of disease progression, and identify a critical step in the development of HF that could be a target for chemotherapy.

  X. Sun , Z. Chen , Y. Li , L. Liao and J. Tan
  Not avialable
 
 
 
Copyright   |   Desclaimer   |    Privacy Policy   |   Browsers   |   Accessibility