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Articles by Y. Laura
Total Records ( 2 ) for Y. Laura
  Y. Laura , Sri Harimurti and Ismaya
  Objective: The objective of this study was to determine the effect of different dimethylacetamide (DMA) levels on sperm quality of Bangkok rooster chicken and sperm survivability in reproductive tract of hen. Materials and Methods: Sperm was collected from 4 Bangkok rooster chicken aged 1 year. Thirty native hens were used for insemination. Sperm from roosters was collected once a week and cryopreserved 24 h in liquid nitrogen containers at -196°C using 3% (P1), 5% (P2), 10% (P3), 14% (P4) and 18% (P5) DMA levels. Frozen thawed sperm quality was observed after thawing at 4° for 60 sec. The hens were slaughtered and sperm viability was recorded in their reproductive tract on 3, 7, 14 and 21 days after insemination. Results: The results showed that freezing decreased the sperm motility, viability and increased abnormalities. The P2 was observed to be the best DMA concentration for chicken sperm cryopreservation. Different DMA concentrations significantly (p<0.01) affected the motility, viability and abnormalities of frozen thawed sperm. Sperm motility (%) after thawing in P1, P2, P3, P4 and P5 were 38.00±9.08, 46.00±8.94, 16.00±5.48, 8.00±2.24 and 1.00±2.74, respectively. Sperm viability (%) after thawing was 40.10±7.21, 53.50±12.53, 22.00±4.43, 15.30±11.40 and 12.00±3.98 in P1, P2, P3, P4 and P5, respectively. Sperm abnormalities (%) after thawing in P1, P2, P3, P4 and P5 were 60.40±7.40, 41.00±3.32, 67.20±4.09, 68.20±8.58 and 71.00±11.64, respectively. In P2, spermatozoa from the hen’s reproductive tract (vagina, uterus, infundibulum and fimbria) were found motile up to 21 days after insemination. Conclusion: It can be concluded that the best DMA concentration for chicken sperm cryopreservation was 5% level in terms of sperm quality and its survivability in the reproductive tract of hen.
  Y. Laura , A. Adyatama , Y. Indra , Y. Achadri and C.M. Airin
  Objective: The aim of this study was to evaluate the estradiol (E2) residues found in milk from local dairy farms of cows in different physiological reproduction states to determine whether the E2 residues were still within normal limits for consumption. Materials and Methods: This research used 22 adult Friesian Holstein Crossbreed (PFH) cows that were divided into 3 groups; A: Productive cows (non-pregnant and non-estrus conditions), B: Pregnant cows in the second trimester of gestation and C: Estrus cows. All of the cows were in the productive age range of 2-4 years old or in the second to fourth parity. Blood and milk samples were collected twice a day. Blood samples were kept at room temperature for 24 h to collect the serum and then stored in a freezer. The milk was centrifuged at 2000 rpm for 10 min and the supernatant was collected and then kept frozen. The estradiol assay was conducted with an ELISA kit from Calbiotech. The body weight was estimated with Rondo measuring tape and the daily milk production was evaluated. The data were analyzed in a completely randomized design with IBM SPSS 23. Results: Reproduction conditions affected the E2 residues in milk (p<0.05). The estradiol residues found in milk were greater than in serum due to an unknown mechanism. The highest E2 residues were observed in the milk of pregnant cows (187.425±27.315 pg mL–1). Conclusion: It can be concluded that theE2 residues found in milk in every group was very low compared with the normal estradiol levels consumed by humans.
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