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Articles by Y. Fujii
Total Records ( 4 ) for Y. Fujii
  T Toyama , H Yamashita , H Sugiura , N Kondo , H Iwase and Y. Fujii
  Objective

The CYP2D6 enzyme plays a major role in converting tamoxifen to its active metabolites. We investigated whether there is an association between the CYP2D6*10 allele and clinical outcome in node-negative Japanese breast cancer patients.

Methods

CYP2D6 genotyping was performed in 154 node-negative breast cancer patients who had received adjuvant tamoxifen treatment alone. The CYP2D6 genotypes were determined using the TaqMan Allelic Discrimination Assay.

Results

Eighteen percent (28 of 154) of the patients carried the CYP2D6*10/*10 genotype, 40% the CYP2D6 wild-type (wt)/*10 genotype and 42% the CYP2D6 wt/wt genotype. There were no discernible correlations between clinicopathologic parameters and the CYP2D6*10 genotype. Next, we determined whether there was a correlation between the CYP2D6*10 genotype and survival and found that the clinical outcome for patients carrying the CYP2D6*10/*10 genotype was similar to those with other genotypes.

Conclusions

Our results suggest that the CYP2D6*10 genotype is unlikely to have any clinical significance for prognosis of node-negative Japanese breast cancer patients receiving adjuvant tamoxifen alone.

  Y. Fujii , S.M.A. Kawsar , R. Matsumoto , H. Yasumitsu , N. Kojima and Y. Ozeki
  To find novel carbohydrate-binding proteins (lectins) from marine invertebrates to understand the binding mechanism of the protein and to apply it for glycan-dependent diagnostics and/or glycoconjugates capture technology. A D-galactoside-binding lectin was purified from foot of bladder moon shell, Glossaulax didyma by lactosyl-agarose affinity chromatography. The crude supernatant by Tris-buffered saline had strong hemagglutination activity against trypsinized and glutaraldehyde-fixed human erythrocyte. However, the activity was not inhibited by any tested saccharides and chilete reagents. On the other hand, the dialyzed crude supernatant obtained from the precipitates with 100 mM lactose in Tris-buffered saline had also hemagglutination activity inhibited by β-galactoside and D-galactose. The lectin was purified with lactosyl-agarose affinity chromatography. The molecular mass of the lectin was determined to be 60 kDa by SDS-PAGE under reducing and non-reducing conditions and being a 60 kDa polypeptide monomer by gel permeation chromatography. The association-rate constant (kass) and dissociation-rate constant (kdiss) determined for the lectin against asialofetuin was determined as 5.4x104 M-1sec-1 and 7.2x10-3sec-1, respectively. Lectin-conjugated Sepharose gel captured asialofetuin and eluted it by lactose-containing buffer from the gel, indicating that the lectin could catch the asialoglycoprotein. It was concluded that a many amount of a D-galactoside-binding lectin which can catch asialoglycoprotein presents in foot of the bladder moon shell.
  S Ohno , N Terada , N Ohno , S Saitoh , Y Saitoh and Y. Fujii
 

Our final goal of morphological and immunohistochemical studies is that all findings examined in animal experiments should reflect the physiologically functional background. Therefore, the preservation of original components in cells and tissues of animals is necessary for describing the functional morphology of living animal organs. It is generally accepted that morphological findings of various organs were easily modified by stopping their blood supply. There had been a need to develop a new preparation technique for freezing the living animal organs in vivo and then obtaining acceptable morphology and also immunolocalization of original components in functioning cells and tissues. We already developed the ‘in vivo cryotechnique’ (IVCT) not only for their morphology, but also for immunohistochemistry of many soluble components in various living animal organs. All physiological processes of cells and tissues were immediately immobilized by IVCT, and every component in the cells and tissues was maintained in situ at the time of freezing. Thus, the ischaemic or anoxic effects on them could be minimized by IVCT. Our specially designed cryoknife with liquid cryogen has solved the morphological and immunohistochemical problems which are inevitable with the conventional preparation methods at a light or electron microscopic level. The IVCT will be extremely useful for arresting transient physiological processes and for maintaining any intracellular components in situ, such as rapidly changing signal molecules, membrane channels and receptors.

 
 
 
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