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Articles by Y. Zafar
Total Records ( 2 ) for Y. Zafar
  Muhammad Aslam , Nadia Iqbal , M. Mohsin Iqbal , M.A. Haq and Y. Zafar
  In the present study, a series of experiment were carried out to investigate the influence of explant type and media combination on the induction of embryogenic callus, plant regeneration and suitable explant for DNA microinjection. The size of explant played major role in the plant regeneration. Mortality rate was high when smaller size explant was used. The plants developed through meristem culture did not undergo any type of malformation because the meristems are highly organized tissues and showed no variation for the phenotypic characters in these studies. With the increase in kinetin concentration up to 2 mg L-1, shoot formation was enhanced and callus initiation and proliferation was observed which was maximum at 6 mg L-1 concentration. Best media for callus induction, shoot and root regeneration was MS medium supplemented with 2 mg L-1 K and 1.5 mg L-1 IAA. All varieties showed higher degree of root formation except FH-900 and CIM-446 that showed least rooting. The MS medium without growth regulators produced moderate shoot and root formation while medium containing different levels of phytohormones effected significantly in shoot and root formation. Charcoal had positive effect on callus induction and browning was reduced. It is concluded that cotyledon and root from the local genotypes are not suitable explants for callus induction and proliferation. Ovules and hypocotyls are the best for callus induction. The efforts are underway to develop plant regeneration procedure for gene transfer leading to rapid introduction of leaf curl virus resistance into the higher yielding cotton genotypes and vice versa.
  S. Mansoor , M. Hussain , S. H. Khan , A. Bashir , A. B. Leghari , G. A. Panwar , W.A. Siddiqui , Y. Zafar and K. A. Malik
  Samples of cotton plants showing symptoms of cotton leaf curl disease were collected from cotton fields in Sindh. Samples of some other plants including tomato, chillies, okra and Hibiscus suspected for whitefly-transmitted geminiviruses were also collected from these areas and total DNA was extracted. Degenerate primers designed to amplify DNA-A of whitefly-transmitted geminiviruses were used in PCR for the amplification of viral DNA. A product of expected (1.4 kb) was obtained from all these samples which confirmed the infection of whitefly-transmitted geminiviruses. PCR primers specific for the two whitefly-transmitted geminiviruses species namely CLCuV-Pk1 and CLCuV-Pk2 found associated with cotton leaf curl disease in Punjab were also used to confirm identity of cotton leaf curl virus in Sindh. A product specific for CLCuV-Pk1 was obtained from all four symptomatic cotton samples. The results showed that cotton samples were infected with CLCuV-Pk1 while CLCuV-Pk2 was not detected in these samples. This is the first report of detection of whitefly-transmitted geminiviruses on these crops from Sindh. Our data not only confirm the presence of a whitefly-transmitted geminivirus on cotton but also showed that the disease is caused by one of the virus species found in Punjab.
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