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Articles by Y. Rassi
Total Records ( 6 ) for Y. Rassi
  M. Khoobdel , M. Shayeghi , H. Vatandoost , Y. Rassi , M.R. Abaei , H. Ladonni , A. Mehrabi Tavana , S.H. Bahrami , M.E. Najaffi , S.H. Mosakazemi , K. Khamisabadi , S. Azari Hamidian and M.R. Akhoond
  In this study, six types of current military uniforms of Iran were treated by permethrin (0.125 mg permethrin [AI] cm-2) and examined against the biting of natural population mosquitoes of rural areas of kazeroon, Fars Province, south of Iran, namely Culex and Anopheles. Eight volunteers were selected for this study. Six of them put on the treated uniforms and the other two ones wore the untreated uniforms. All the subjects participated in the night biting test for eight active nights of July and August 2004. There was no significant difference in the protection of different treated uniforms against mosquitoes biting. In this study, the average number of biting among who wore untreated uniforms (controls) was 3.21 mosquito biting/min/person (192.8 h-1). This amount was 0.26 (15.6 h-1) for who wore treated uniforms (cases). The relative protection level of treated uniforms, in comparison with untreated ones, was about 91.9%. The protection percent of treated uniforms for different species of mosquitoes, in comparison with untreated ones, was calculated through determining the species of captured mosquitoes, separately from case and control subjects. The results showed that the relative protection percent of treated uniforms against species of Culex bitaeniohynchus, Cx. tritaeniorhynchus, Cx. perexiguus, Cx. theileri and An. stephensi was 72.7, 87, 89.8, 84.3 and 78.7, respectively. The results of chemical analysis with High Performance Thin Layer Chromatography (HPTLC) method, before and after field test, showed that in two weeks of study, there hasn`t been any significant decrease in the amount of uniforms` permethrin.
  H. Vatandoost , R. Abdoljabari Boonab , M.R. Abai , M.A. Oshaghi , Y. Rassi , S. Gholizadeh , K. Mashhadi-Esmail A. Kousah , M. Haghi , M. Gorghani , B. Aliakbarie-Sharabiani , M. Seif Farshid and N. Piazak
  An entomological survey was carried out on the bionomics of Anophelines species in Kalibar, East-Azerbaijan during the year 2003-2004. The result showed that five Anophelines species comprising A. sacharovi, A. maculipennis, A. superpictus, A. hyrcanus and A. claviger were present in the study area. Based on the species density rate it was assumed that A. sacharovi could play an important role in malaria transmission and A. maculipennis and A. superpictus could be considered as secondary vectors. A. sacharovi was active from May to September which peaks in July and August in outdoor and indoor, respectively. This species was mainly endophil and anthropophil. Biting activity of A. sacharovi took place in the second half of night. Susceptibility tests using the WHO-recommended discriminative doses of insecticides revealed that this species is resistant to DDT, tolerant to dieldrin and susceptible to malathion, fenitrothion, permethrin, and deltamethrin. A. maculipennis had high density at the beginning of summer, then its population fell down slowly and in early autumn reached to the lowest. The population of this species was found frequently in human shelters and most of its bites took place in the second half of night. Its larvae occurred in slow flowing water and channels with water plants and were more abundant in August.
  Y. Rassi , E. Javadian , A. Nadim , A. Zahraii , H. Vatandoost , H. Motazedian , K. Azizi and M. Mohebali
  Visceral leishmaniasis is one of the most important health problems in Iran which is transmitted by sandflies species to human. In order to determine vector/s of the disease an investigation was carried out in Meshkinshahr district, the most important focus of the disease in Ardebil province, north west of Iran, during 2001-2005. Using sticky papers, CDC light traps and aspirators, totally 2500 sandflies were collected and identified at the species level. Host blood preference and natural infection of female specimens to promastigotes were tested, respectively using ELISA and dissection followed by PCR assay using species-specific kinetoplast minicircle primers. Results showed that six species of Phlebotomus kandelakii, P. perfiliewi transcaucasicus, P. papatasi, P. jacusieli, P. caucasicus and P. sergenti were present in the district, where P. kandelakii with 40% was the most prevalent species. Anthropophilic index of P. kandelakii species was calculated 32.8%, indicating a strong preference to human. Among the dissected female sandflies, only 11 out of 1002 (1.1%) of P. kandelakii was found naturally infected with promastigotes. Species-specific amplification of the Giemsa stained promastigote slides revealed specific PCR products of Leishmania infantum DNA in the infected P. kandelakii sand-flies. Having found high prevalence and anthropophic index and natural infection to Leishmania infantum provide enough evidences to incriminate the species of P. kandelakii as the main and proven vector of visceral leishmaniasis for the first time in the region and in science.
  M.A. Abaii , Y. Rassi , H. Imamian , M. Fateh , M. Mohebali , S. Rafizadeh , H. Hajjaran , K. Azizi and M. Ismaiili
  A study was made in rural region of Shahrood city, Semnan province in the central of Iran during 2005 to investigate of vectors of zoonotic cutaneous leishmaniasis. Sticky traps and an aspirator were used for collecting of sandflies. Three species of Phlebotomus papatasi, P. caucasicus and Sergentomyia sintoni were collected and identified and the first species was dominant (53%). Nested PCR method were employed for identifying of isolated parasites of dissected female of sandflies. Among the dissected sand flies 3 out of 24 (12.5%) Phlebotomus papatasi and 2 out of 48 (4.2%) Phlebotomus caucasicus were found naturally infected with promastigotes. Species-specific amplification of Giemsa staind promastigote slides revealed specific PCR production of Leishmania major DNA in the infected P. papatasi and P. caucasicus sand flies. Having high prevalence and infection rate provide enough evidence to incriminate of P. papatasi as the main and proven vector of cutaneous leishmaniasis to human in the region and the species of P. caucasicus play the second role for maintenance of disease between rodents.
  H. Nasirian , H. Ladonni , M. Shayeghi , H. Vatandoost , Y. Rassi , M.R. Yaghoobi Ershadi , J. Rafinejad and H.R. Basseri
  The duration of fipronil WHO glass jar method toxicity against twelve strains of feral German cockroaches, Blattella germanica (L.), was determined. In the WHO glass jar bioassay, the average LT50 of susceptible strain was 16.4,14.3,12.4 and 11.3 exposure minutes after 24,48,72 and 96 h, respectively and the average LT95 was 20.3,19.9,19.5 and 19.1 exposure minutes after 24,48,72 and 96 h, respectively. As with the susceptible reference strain, where LT50 was halved from 24 to 96 h, the LT50 of fipronil decreased with time in the feral German cockroach strains. LT50 varied > 8-folds from 16.2 to 24.7 exposure min at 24 h, 8.4 folds from 14.4 to 22.8 min at 48 h and almost 8.8 folds from 12.5 to 21.3 exposure minutes at 72 h. At the end of the bioassay at 96 h, LT50 varied from 11.6 to 19.7 exposure minutes, which is 1.0 and 1.7 folds exposure min higher than the standard susceptible value of fipronil. All German cockroach strains showed a similar susceptibility or very low tolerance (1.5 to 1.7 folds) to fipronil compared with the susceptible laboratory strain and the steep slopes of time exposure-mortality curves indicated that the feral German cockroach strains was homogenous in time exposures to fipronil. These results indicate that the fipronil was relatively slow-acting in WHO glass jar method bioassay, with LT50 values decreasing until 96 h and becoming stable thereafter.
  B. Davari , H. Vatandoost , H. Ladonni , M. Shaeghi , M.A. Oshaghi , H.R.Basseri , A.A. Enayati , Y. Rassi , M.R. Abai , A.A. Hanfi Bojd and K. Akbarzadeh
  Using of insecticides depend largely on the knowledge of the susceptibility levels of malaria vectors to these chemical. In this study, the susceptibility levels of Anopheles stephensi to DDT 4%, dieldrin 0.4%, fipronil 1%, deltamethrin 0.05%, permethrin 0.75%, lambdacyhalothrin 0.05% and cyfluthrin 0.15% were determined according to WHO methods in three endemic malarious regions; Sistan and Baluchistan, Hormozgan and Fars provinces. The LT50 values of different insecticides were calculated using the probit regression line for each strain. The results showed that An. stephensi is resistant to DDT, dieldrin and fipronil in all regions; however Bandar Abbas strain showed the highest level of resistance to these three insecticides. All the tested strains were sensitive against four pyrethroid insecticides. The LT50 of Bandar Abbas strain against cyfluthrin and deltamethrin with 5.46 and 3.22 min were the highest and the lowest values respectively. The highest and the lowest mortality rates of the Kazeroon strain of An. stephensi were against lambdacyhalothrin and permethrin with 6.80 and 0.682 min, respectively. In Iranshahr region, the figures for deltamethrin, permethrin, lambdacyhalothrin and cyfluthrin were 3.50, 5.10, 3.95 and 5.38 min, respectively. The results of the study suggested a possible cross-resistance between dieldrin and fipronil. Regarding the sensitivity of An. stephensi to different pyrethroids in the malarious regions of Iran, using pyrethroids is still suggested. Performing complementary tests with cyfluthrin on Bandar Abbas strain and with permethrin on Iranshahr strain seems to be necessary.
 
 
 
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