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Articles by Y Yamamoto
Total Records ( 8 ) for Y Yamamoto
  T Ishii Yonemoto , H Masuzaki , S Yasue , S Okada , C Kozuka , T Tanaka , M Noguchi , T Tomita , J Fujikura , Y Yamamoto , K Ebihara , K Hosoda and K. Nakao

Increased expression and activity of the intracellular glucocorticoid-reactivating enzyme 11β-hydroxysteroid dehydrogenase type 1 (11β-HSD1) contribute to dysfunction of adipose tissue. Although the pathophysiological role of 11β-HSD1 in mature adipocytes has long been investigated, its potential role in preadipocytes still remains obscure. The present study demonstrates that the expression of 11β-HSD1 in preadipocyte-rich stromal vascular fraction (SVF) cells in fat depots from ob/ob and diet-induced obese mice was markedly elevated compared with lean control. In 3T3-L1 preadipocytes, the level of mRNA and reductase activity of 11β-HSD1 was augmented by TNF-, IL-1β, and LPS, with a concomitant increase in inducible nitric oxide synthase (iNOS), monocyte chemoattractant protein-1 (MCP-1), or IL-6 secretion. Pharmacological inhibition of 11β-HSD1 and RNA interference against 11β-HSD1 reduced the mRNA and protein levels of iNOS, MCP-1, and IL-6. In contrast, overexpression of 11β-HSD1 further augmented TNF--induced iNOS, IL-6, and MCP-1 expression. Moreover, 11β-HSD1 inhibitors attenuated TNF--induced phosphorylation of NF-B p65 and p38-, JNK-, and ERK1/2-MAPK. Collectively, the present study provides novel evidence that inflammatory stimuli-induced 11β-HSD1 in activated preadipocytes intensifies NF-B and MAPK signaling pathways and results in further induction of proinflammatory molecules. Not limited to 3T3-L1 preadipocytes, we also demonstrated that the notion was reproducible in the primary SVF cells from obese mice. These findings highlight an unexpected, proinflammatory role of reamplified glucocorticoids within preadipocytes in obese adipose tissue.

  Y Yamamoto , S Yamazaki , Y Hayashino , O Takahashi , Y Tokuda , T Shimbo , T Fukui , S Hinohara , Y Miyachi and S. Fukuhara

Objective  To evaluate the relationship between frequency of pruritic symptoms experienced over a 1-month period and psychological stress.

Design  Cohort study.

Setting  Population-based study in Japan.

Participants  A total of 2224 participants at least 18 years old and without psychiatric disorders participated in the Japan Health Diary Study (October 2003), a cohort study comprising a representative sample in Japan.

Main Outcome Measures  Frequency of pruritic symptoms assessed by self-reported health diaries over the 1-month period and subsequent psychological stress measured using the Japanese version of the Perceived Stress Scale.

Results  The 2224 participants had a mean age of 44.6 years, 1212 (54.5%) were women, and 70 (3.1%) presented with pruritic symptoms. Multivariable analysis showed that patients with pruritic symptoms had significantly higher psychological stress than those without pruritic symptoms (β coefficient, 2.33; 95% confidence interval [CI], 0.53-4.14; P = .01). Furthermore, a linear trend was observed between increased psychological stress and increased severity of pruritic symptoms, with β coefficients for the first, second, and third tertiles for symptoms of 0.81 (95% CI, –1.97 to 3.59), 1.77 (95% CI, –0.82 to 4.37), and 4.86 (95% CI, 1.29 to 8.43), respectively (P value for trend, .004).

Conclusion  Our results suggest that frequency of pruritic symptoms is associated with psychological stress in the general population.

  Y Kimura , Y Ishibashi , E Tsuda , Y Yamamoto , H Tsukada and S. Toh

A high incidence of anterior cruciate ligament (ACL) injuries related to sports activities has been reported; however, the injury situation of ACL injury in badminton has not been elucidated. This study investigated the mechanism of ACL injury in badminton using a questionnaire.


Information on injury mechanism was gathered from interviews with six male and 15 female badminton players who received a non-contact ACL injury playing badminton and underwent ACL reconstruction.


The most common injury mechanism (10 of 21 injuries) was single-leg landing after overhead stroke. Nine of 10 players had injured the knee opposite to the racket-hand side. The second most frequent injury mechanism (eight of 21 injuries) was plant-and-cut while side-stepping or backward stepping. All eight players injured the knee of the racket-hand side. Eleven injuries occurred in the rear court, and six of the 11 injuries occurred during single-leg landing after an overhead stroke.


The knee opposite to the racket-hand side tended to sustain the ACL injuries during single-leg landing after a backhand overhead stroke, whereas the knee of the racket-hand side tended to be injured by plant-and-cut during side or backward stepping. These injury patterns appear to be due to specific movements during badminton.

  Y Yamamoto , T Tanahashi , T Kawai , S Chikahisa , S Katsuura , K Nishida , S Teshima Kondo , H Sei and K. Rokutan

Caloric restriction (CR) is an effective method for prevention of age-associated diseases as well as overweight and obesity; however, there is controversy regarding the effects of dieting regimens on behavior. In this study, we investigated two different dieting regimens: repeated fasting and refeeding (RFR) and daily feeding of half the amount of food consumed by RFR mice (CR). CR and RFR mice had an approximate 20% reduction in food intake compared with control mice. Open field, light-dark transition, elevated plus maze, and forced swimming tests indicated that CR, but not RFR, reduced anxiety- and depressive-like behaviors, with a reduction peak on day 8. Using a mouse whole genome microarray, we analyzed gene expression in the prefrontal cortex, amygdala, and hypothalamus. In addition to the CR-responsive genes commonly modified by RFR and CR, each regimen differentially changed the expression of distinct genes in each region. The most profound change was observed in the amygdalas of CR mice: 884 genes were specifically upregulated. Ingenuity pathway analysis revealed that these 884 genes significantly modified nine canonical pathways in the amygdala. -Adrenergic and dopamine receptor signalings were the two top-scoring pathways. Quantitative RT-PCR confirmed the upregulation of six genes in these pathways. Western blotting confirmed that CR specifically increased dopamine- and cAMP-regulated phosphoprotein (Darpp-32), a key regulator of dopamine receptor signaling, in the amygdala. Our results suggest that CR may change behavior through altered gene expression.

  E Tsuda , Y Ishibashi , A Fukuda , Y Yamamoto , H Tsukada and S. Ono

Several laboratory studies have pointed out a potential risk of femoral tunnel misplacement in anterior cruciate ligament reconstruction with a transtibial technique. The tunnel malposition away from the anatomic attachment may result in increased postoperative knee laxity in double-bundle reconstruction.


This study was conducted to evaluate the femoral and tibial tunnel positions in transtibial double-bundle reconstruction, and to determine the relationship between the tunnel positions and the results of the postoperative knee laxity examinations.

Study Design

Case series; Level of evidence, 4.


Fifty-three of 71 patients who underwent transtibial double-bundle reconstruction from 2004 to 2005 were followed more than 24 months. The tunnel positions for the anteromedial and posterolateral grafts were measured using 3-dimensional computed tomography images applying the quadrant method. The postoperative knee laxity was examined with the KT-1000 arthrometer manual maximum test, anterior drawer test, and pivot-shift test.


The deep-shallow position (parallel to Blumensaat’s line) and high-low position (perpendicular to Blumensaat’s line) of the femoral tunnels were 27.7% ± 5.6% from the most posterior condylar contour and 16.3% ± 5.2% from Blumensaat’s line for the anteromedial graft, and 35.5% ± 6.4% and 48.0% ± 5.4% for the posterolateral graft. The medial-lateral and anterior-posterior positions of the tibial tunnels were 46.1% ± 2.6% from the most medial contour and 36.5% ± 4.9% from the most anterior contour for the anteromedial graft, and 47.5% ± 3.1% and 51.6% ± 5.0% for the posterolateral graft. There was no statistical correlation between any parameters of the femoral or tibial tunnel position and the results of the knee laxity tests.


The femoral tunnels placed in transtibial double-bundle reconstruction were located appropriately in high-low and deep-shallow orientation, but had larger variability than the previously reported data of the anatomic femoral attachment. However, the variability of the femoral tunnel position was not so large as to result in graft insufficiency with increased postoperative knee laxity.

  S. K Min , K Nakazato , Y Yamamoto , K Gushiken , H Fujimoto , H Fujishiro , Y Kobayakawa and K. Hiranuma

Background: The authors previously identified a significant association between lumbar disc degeneration (LDDG) and cartilage intermediate layer protein (CILP) single nucleotide polymorphism (SNP) in collegiate male judokas.

Hypothesis: A significant association between LDDG and the CILP SNP is observed in Japanese collegiate athletes.

Study Design: Cross-sectional study; Level of evidence, 3.

Methods: The participants were 601 trained collegiate athletes (male, 403; female, 198) from 7 different sports. Lumbar disc degeneration was evaluated using T2-weighted magnetic resonance imaging. Genotyping of the CILP gene (1184T/C) was performed by using DNA sequencing.

Results: Among the 601 collegiate athletes, the odds ratio (OR) for the occurrence of LDDG with the CILP C allele was 1.4 (95% confidence interval [CI], 1.05-1.86). By using logistic regression analysis concomitant with the interaction term and the Wald test, the authors found that weight (OR, 1.04; 95% CI, 1.02-1.06), CILP genotype (CT: OR, 2.0; 95% CI, 1.24-3.15; CC: OR, 2.9; 95% CI, 1.09-7.74), and gender (OR, 2.1; 95% CI, 1.21-3.67) were significant risk factors for LDDG. These analyses also indicated that there was no effect of the CILP genotype on LDDG in female athletes.

Conclusion: The CILP SNP 1184T/C is a risk factor for male collegiate athletes. Information regarding the CILP gene polymorphism may be important for preventing and managing lumbar disc diseases, especially in male athletes.

  K Ohe , T Watanabe , S. i Harada , S Munesue , Y Yamamoto , H Yonekura and H. Yamamoto

Receptor for advanced glycation endproducts (RAGE) is a cell-surface receptor. The binding of ligands to membrane-bound RAGE (mRAGE) evokes cellular responses involved in various pathological processes. Previously, we identified a novel soluble form, endogenous secretory RAGE (esRAGE) generated by alternative 5' splice site selection in intron 9 that leads to extension of exon 9 (exon 9B). Because esRAGE works as an antagonistic decoy receptor, the elucidation of regulatory mechanism of the alternative splicing is important to understand RAGE-related pathological processes. Here, we identified G-rich cis-elements within exon 9B for regulation of the alternative splicing using a RAGE minigene. Mutagenesis of the G-rich cis-elements caused a drastic increase in the esRAGE/mRAGE ratio in the minigene-transfected cells and in loss of binding of the RNA motif to heterogenous nuclear ribonucleoprotein (hnRNP) H. On the other hand, the artificial introduction of a G-stretch in exon 9B caused a drastic decrease in the esRAGE/mRAGE ratio accompanied by the binding of hnRNP H to the RNA motif. Thus, the G-stretches within exon 9B regulate RAGE alternative splicing via interaction with hnRNP H. The findings should provide a molecular basis for the development of medicines for RAGE-related disorders that could modulate esRAGE/mRAGE ratio.

  K. R Long , Y Yamamoto , A. L Baker , S. C Watkins , C. B Coyne , J. F Conway and M. Aridor

While dynamin pinches vesicles from the plasma membrane, the Sar1 GTPase specializes in cinching ER membrane tubules.

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