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Articles by Xuewei Li
Total Records ( 9 ) for Xuewei Li
  Xun Wang , Haifeng Liu , Ling Zhao , Han Dong , Yuhao Wang , Di Sun , Yuyin Liu , Ruqi Yang and Xuewei Li
  To study the effects of Crude Polysaccharides from Catathelasma ventricosum (CVCP) on proliferation and differentiation of 3T3-L1 preadipocyte. 3T3-L1 cells were treated with culture media contained different concentrations of CVCP (0, 150, 300 and 600 μg mL-1) for 24 h and subsequently cell proliferation was assayed by MTT Method. The extent of the differentiation was determined by fluescence spectrophotometry and Nile Red staining on days 8 after treatment with culture media supplemented with different concentration CVCP (0, 150, 300 and 600 μg mL-1). As the results, the different concentration CVCP (150, 300 and 600 μg mL-1) promoted proliferation of 3T3-L1 preadipocytes when compared with the control (p<0.05). And it stimulated cell differentiation into adipocyte at low concentration (150 μg mL-1) while it inhibited cell differentiation into adipocyte at higher concentration (300 and 600 μg mL-1). But the difference was not significant (p>0.05). Besides, lipid content in 3T3-L1 cells treated with 150 μg mL-1 was significantly higher than treated with 600 μg mL-1 (p<0.05). It can be concluded from the results that the CVCP promoted proliferation of the 3T3-L1 cells 24 h after treatment. Meanwhile, it was involved to some extent in regulation of adipogenesis and lipogenesis.
  Xun Wang , Haifeng Liu , Ling Zhao , Han Dong , Zhengxin Yan , Hengmin Cui and Xuewei Li
  Constitutive Androstane Receptor (CAR) is one of members of the nuclear receptors superfamily which is involved in induction of drug metabolism and lipogenic pathway. In this study, the reseachers investigated whether the mRNA expression pattern of CAR in porcine preadipocyte is changed during the process of induced differentiation. Preadipocytes were aseptically taken from 3 days old piglet under general anesthesia and grown to near confluence. Postconfluent cells (Day 0) were further cultured in differentiation medium for 3 days. From day 3 onward, the cells were cultured in maintain-medium based on differentiation medium without roglitazone and IBMX until day 15. The total RNA was isolated on seven time points (i.e., days 0, 2, 4, 6, 8, 10 and 15). The mRNA expression changes of the CAR before and after porcine primary preadipocytes differentiation that acorss seven time points (i.e., days 0, 2, 4, 6, 8, 10 and 15) were measured using the quantitative RT-PCR method. In addition, the researchers also examined lipid accumulation and the mRNA expression of adpocyte differentiation markers (Such as PPARγ and RXRα) which confirmed the differentiation of preadipocytes to adipocytes. The results showed that the mRNA expression of CAR was up-regulated during porcine primary preadipocyte differentiation. There is the reason that the CAR may be involved in the process of preadipocyte differentiation and is a potential drug target for the treatment of obesity and diabetes.
  Mingwang Zhang , Mingzhou Li , Long Jin , Yan Li and Xuewei Li
  Tibetan pig is an endangered plateau type pig breed which distributed mainly in Qinghai-Tibetan Plateau. In present study, researchers analyzed 218 prior published partial mitochondrial D-loop sequences (415 bp) by using phylogenetic, network, mismatch distribution and Analysis of Molecular Variance (AMOVA) analysis to clarify the population differentiation and explore the relationships among populations from different geographical locations of Tibetan pigs. The results revealed a total of 43 haplotypes in 218 samples from seven Tibetan pig populations in which only one common Haplotype (H1) was shared by the seven populations. Haplotype diversity was high (0.889) whereas nucleotide diversity among all individuals was low (0.00534). It is notable that Hezuo Tibetan pigs have 19 unique haplotypes in total 24 haplotypes of 53 individuals with the highest nucleotide diversity (0.00765). Bayesian tree and median joining network procedures carried out on the data showed that seven Tibetan pig haplotypes were essentially indistinguishable except some unique haplotypes from Hezuo. The AMOVA analysis indicated that the genetic variation mainly occurred within populations (85.41%) but variance among populations was only 14.59%. The unimodal mismatch distribution of haplotypes, together with significant negative values of Fu’s FS and negative values of Tajima’s D suggest that a recent population expansion of Tibetan pig has been occurred in Diqing, Hezuo and total population.
  Lu Bai , Jideng Ma , Yue Wang , Zhengjian Zhao , Xuewei Li , Anan Jiang and Lin Bai
  Blood plays an important role in many biological processes and circulating microRNAs (miRNAs) in the blood have been reported to be biomarkers for various physiological and pathological states. However, few studies have examined porcine blood miRNAs, especially arterial blood miRNAs. Here, researchers sequenced the microRNAomes of porcine arterial and venous blood samples in which we identified 277 and 294 known porcine miRNAs, respectively. In both blood samples, highly expressed miRNAs were implicated in erythropoiesis and angiogenesis. A total of 57 miRNAs showed significantly different expression levels (p<0.01) between arterial and venous blood of which seven were enriched in arterial blood and five were enriched in venous blood by more than a 1.5 fold change. Interestingly, functional enrichment analysis of the genes predicted to be targeted by the enriched miRNAs indicated that arterial blood was mainly associated with the immune response and venous blood was primarily associated with apoptosis and the hypoxia response. The results suggest that the 12 miRNAs with differential expression should be considered as biomarkers for physiological differences between arterial and venous blood and that they may help promote the further molecular diagnosis of blood.
  Ya Tan , Jideng Ma , Yingkai Liu , Keren Long , Liujun He , Xuewei Li and Li Zhu
  MicroRNAs (miRNAs) are non-coding RNAs that negatively regulate gene expression. Compelling evidences have demonstrated the important roles of miRNAs in muscle proliferation, differentiation and development processes. Cardiac muscle and skeletal muscle (longissimus dorsi muscle and psoas major muscle) are both striated muscles which perform different functions in vivo. However, little is known about the differences of miRNA expression pattern between these distinct muscles. In this study, researchers applied a deep sequencing approach to identify the miRNA transcriptomes between cardiac and skeletal muscles. Three sequencing libraries were constructed from three 210 days old pigs, a total of 56 M raw data was generated with 95.58% of them were mappable sequences. In total, 321 unique miRNAs were identified in three sequencing libraries of which 109 were differentially expressed. Ranking analysis showed that six out of the ten most highly expressed miRNAs in cardiac muscle were up-regulated compared with longissimus dorsi muscle and psoas major muscle. GO and KEGG pathway analyses for the predicted target genes of the six up-regulated miRNAs showed that the miRNAs highly expressed in CM are mainly involved in insulin and calcium signaling pathways and the constructed miRNA-mRNA interaction networks highlighted the important differences between cardiac and skeletal muscles, all these results may promote the researches on muscle-related diseases in a certain degree.
  Yihui Liu , Jideng Ma , Li Chen , Pengbo Lou , Jun Zhou , Mingzhou Li and Xuewei Li
  High-throughput sequencing of two small RNA libraries derived from immature (20 days old) and mature (210 day old) porcine testis samples yielded over 20 million high-quality reads. Researchers detected 461 mature microRNAs (miRNAs) encoded by 277 precursor (pre)-miRNAs of which 428 were unique. In total, 303 unique miRNAs of (428, 70.79%) were differentially expressed between immature and mature porcine testes. Compared with immature testis, 95 unique miRNAs were up-regulated and 208 unique miRNAs were down-regulated in mature testis. Strikingly, researchers found that most miRNAs and differentially expressed miRNAs were preferentially located on the X chromosome which implied their crucial roles in the sex-determination system. Furthermore, GO and KEGG analyses of the target genes that were predicted from the highly abundant differentially expressed miRNAs between mature and immature porcine testes illustrate the likely roles for these miRNAs in spermatogenesis. The study indicates that miRNAs are extensively involved in spermatogenesis and that unraveling miRNA functions in the testis will further the understanding of regulatory mechanisms of mammalian spermatogenesis and male infertility treatment.
  Jian Xiao , Jideng Ma , Long Jin , Xuewei Li , Mingzhou Li and An-An Jiang
  The liver is a vital organ that plays a major role in metabolism and other functions in the body. In this study, researchers used microarray analyses to investigate the gene expression profile during the development of the liver in pigs (Suss crofa). Researchers identified various differentially expressed genes in the liver between the prenatal and postnatal stages. The upregulated genes in the prenatal liver were related to the cell cycle and developmental process. The genes that were upregulated in the postnatal liver were active in the immunological process. The study presented gene expression profiles of the porcine liver during the pre and post-natal stages which contribute to the understanding porcine liver development.
  Shen He , Chong Feng , Chuan Long , Hongmei Pan , Ning Wang , Ningning Shi , Fei Wang , Yanrong Zhou , Hongxing Chen , Mingxing Chu , Dengke Pan and Xuewei Li
  The development of α-1,3-galactosyltransferase gene knockout pigs along with overexpression of human Complement Regulatory Proteins (hCRPs) has been an important step in overcoming hyperacute rejection when pig organs are transplanted into nonhuman primates. Most previous studies have used commercial pigs as organ donors. However, little has been reported about the use of inbred miniature pigs as genetic donors expressing hCRPs. As expression of hCRP on the surface of pig cells, especially human membrane cofactor protein (hCD46) could effectively protect them against human complement-mediated rejection, researchers constructed an engineered hCD46 minigene expression vector and generated transgenic Wuzhishan miniature Pigs (WZSP) expressing hCD46. Expression of hCD46 with a distinct tissue-specific pattern similar to the human one was observed using immunohistochemistry. Various tissues revealed strong immunostaining in most epithelial tissues and vascular endothelium. Flow cytometry analysis of hCD46 porcine AECs showed expression levels similar to human AECs. Complement-mediated cytolysis of transgenic porcine Aortic Endothelial Cells (AECs) with human serum showed significant protection. Distinct differences between ABO blood groups were also observed with much less cytolysis of porcine AECs treated with B blood group serum than with other serum types. In conclusion, transgenic WZSPs with sufficient expression of hCD46 have been established successfully and will facilitate further xenotransplantation research.
  Xun Wang , Haifeng Liu , Ling Zhao , Han Dong , Chunyou Ning , Xuewei Li and Hengmin Cui
  To study the effects of crude polysaccharides from Poria cocos (PCCP) on proliferation and differentiation of 3T3-L1 preadipocyte. 3T3-L1 cells were treated with culture medium contained different concentrations of PCCP (0, 50, 100 and 200 μg mL-1) for 48 h and subsequently cell proliferation was assayed by MTT Method. The extent of the differentiation was determined by fluescence spectrophotometry and Oil Red O staining on days 8 after treatment with culture medium supplemented with different concentration PCCP (0, 50, 100 and 200 μg mL-1). As the results, the different concentration PCCP (50, 100 and 200 μg mL-1) inhibited to some extent proliferation of 3T3-L1 preadipocytes when compared with the control. But the difference is not significant (p>0.05). And PCCP stimulated cell differentiation into adipocyte at lower concentration (50 and 100 μg mL-1) (p<0.05) while it inhibited cell differentiation into adipocyte at high concentration (200 μg mL-1) (p>0.05). It can be concluded from the results that the PCCP was involved in regulation of adipogenesis and lipogenesis.
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