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Articles by Xue-Wei Li
Total Records ( 6 ) for Xue-Wei Li
  An-An Jiang , Ming-Zhou Li , Kai Zhang , Yi-Ren Gu and Xue-Wei Li
  PPARGC1A, the gene encoding peroxisome proliferative activated receptor-γ coactivator-1α is a useful candidate gene for pork quality due to its regulation of the determination of myofibre type. In this study, changes in developmental gene expression of PPARGC1A in longissimus dorsi muscles were examined in two pig breeds, Landrace (lean) and Meishan pigs (obese) at birth, 30, 60, 90, 120 and 150 days of age using quantitative real-time PCR. The results indicated that there were distinct expression patterns of PPARGC1A in the two pig breeds. At most stages, except at 30 and 60 days, the abundance of PPARGC1A mRNA in Meishan pigs was significantly higher than that in Landrace (p<0.01). Interestingly, there was a negative correlation between the PPARGC1A expression pattern and myofibre cross-sectional area across five developmental stages which implies that PPARGC1A might have an important influence on myofibre growth.
  Jiu-Qiang Guan , Ming-Zhou Li , Ping-Er Lou , Su-Rong Shuai , Qing Zhao and Xue-Wei Li
  The three adiponectin-related genes (AdipoQ, AdipoR1, AdipoR2) have been notably identified in association with adiponectin levels in vivo and obesity phenotypes. The mRNA levels of AdipoQ, AdipoR1 and AdipoR2 have been measured across six different adipose tissues from the female leaner Landrace and female fatty Rongchang pig breeds using quantitative real time RT-PCR (q-PCR) approach. The mRNA levels of AdipoQ (PB = 2.27x10-9), AdipoR1 (PB = 0.04) and AdipoR2 (PB = 5.72x10-6) were higher in the leaner Landrace pigs than in the fatty Rongchang pigs. The mRNA levels of AdipoQ (PT (VATs vs. SATs) = 2.67x10-3) and AdipoR2 (PT (VATs vs. SATs) = 2.27x10-4) were higher in SATs compared with VATs. These results present the breed and tissue-specific expression patterns of AdipoQ, AdipoR1 and AdipoR2 which highlight their potential as candidate genes for the pig fat mass trait.
  De-Bing Li , Chao Li , Shan-Shan Shao , Gong-Wei Zhang , Yong Tang , Ding-Gang Zhou and Xue-Wei Li
  To investigate the effects of dietary Vitamin D3 (VD3) on MHC-II-β gene expression in Monopterus albus (M. albus). A total of 540 healthy M. albus (weigh, 21.7±2.1 g) were randomly assigned to six groups (3 replicates per group, 30 M. albus per replicate) and fed with dietary VD3 at various concentrations (0 (control), 250, 500, 1,000, 2,000 and 4,000 IU kg-1). After 20, 40 and 60 days of feeding, researchers randomly selected six M. albus from each group and collected tissues (hepatopancreas, spleen, head kidney and hindgut) for detection of MHC-II-β gene expression using real-time quantitative PCR. MHC-II-β was expressed in four tissues with expression in the head kidney significantly higher (p<0.01) than that in the spleen, hindgut and hepatopancreas. At 20 days, the highest MHC-II-β expression was detected in the 4,000 IU kg-1 group in the head kidney and in the 2,000 IU kg-1 group in the spleen, both of which were significantly higher than that of the other groups (p<0.05). At 40 days, the 1000 IU kg-1 group showed the highest MHC-II-β expression in the head kidney, hindgut and hepatopancreas. At 60 days, the 500 IU kg-1 group showed the highest MHC-II-β expression in the head kidney and spleen and was significant higher than that of the control (p<0.05) while the 4,000 IU kg-1 group showed significantly lower MHC-II-β expression compared to the control (p<0.05). Relative to the MHC-II-β expression in the head kidney, the results demonstrate that short-term (20 days) and high dose dietary provision of VD3 (4,000 IU kg-1) significantly increased MHC-II-β expression in M. albus immune organs. However, the highest MHC-II-β expression was observed with long-term (60 days) dietary provision of VD3 at a dose of 500 IU kg-1.
  Ping-Er Lou , Ying-Kai Liu , Tao Wang , Ming-Zhou Li and Xue-Wei Li
  Monozygotic (MZ) twins having the same DNA information have been used for many biomedical studies. Pigs present high similarity with humans and have been used as an ideal mammalian model the use of MZ twins is even more valuable. In the study, researchers present a systematic method for the stepwise exclusion of inconsistently genotyped individuals in a large population by Short Tandem Repeat (STR) genotyping. Five pairs of microsatellite markers with different fluorescent dyes were selected and their reliability was tested. The results demonstrate that the cumulative exclusion rate of all markers was 99.67% in 1,201 pigs; two pairs of MZ twins were initially identified. Researchers have established an effective, easy and cheap way to identify MZ twin pigs.
  De-Bing Li , Shan-Shan Shao , Gong-Wei Zhang , Ding-Gang Zhou and Xue-Wei Li
  To investigate the effects of dietary Vitamin D3 (VD3) on the apoptosis, morphological structure and antibacterial peptide hepcidin gene expression in the spleen of Monopterus albus (M. albus), so as to provide theoretical basis for investigating the immunoregulatory functions and mechanisms of VD3. A total of 540 healthy M. albus (weigh, 21.7±2.1 g) were randomly assigned into six groups (3 replicates per group, 30 M. albus per replicate) and fed with dietary VD3 at various concentrations (0 (control), 250, 500, 1000, 2000 and 4000 IU kg-1). The experiment lasted for 60 days. After feeding experiment, the spleen was collected from 6 M. albus selected randomly from each group for the detection of apoptosis using FCM and antibacterial peptide hepcidin gene using fluorescent quantitative RT-PCR, besides morphological structure was observed using histological method. The Proliferation Index (PI) was significantly increased (p<0.05), the apoptotic rate was significantly reduced (p<0.05), hepcidin gene expression was significantly up-regulated (p<0.05), the percentage of white pulp was increased and more splenic corpuscles were clearly visible in the 1000 IU kg-1 group compared to the control group. There were no significant differences in hepcidin gene expression in the 4000 IU kg-1 group and the number of splenic corpuscle was reduced, gradually degenerating and disappearing. The results demonstrate that appropriate dose dietary provision of VD3 significantly reduced the cell apoptosis rate in the spleen of M. albus whereas promoted the growth of peripheral lymphoid organ and significantly increased hepcidin gene expression, enhancing immunity. However, when the dietary provision of VD3 was excessive or deficient, the apoptosis was increased, cell proliferation was blocked, growth of spleen was inhibited, hepcidin gene expression was down-regulated and immune function was impaired.
  De-Bing Li , Chao Li , Shan-Shan Shao , Gong-Wei Zhang , Yong Tang , Ding-Gang Zhou and Xue-Wei Li
  To investigate the effects of dietary Vitamin D3 (VD3) on MHC-II-β gene expression in Monopterus albus (M. albus). A total of 540 healthy M. albus (weight, 21.7±2.1 g) were randomly assigned to six groups (3 replicates per group, 30 M. albus per replicate) and fed with dietary VD3 at various concentrations (0 (control), 250, 500, 1,000, 2,000 and 4,000 IU kg-1). After 20, 40 and 60 days of feeding, researchers randomly selected six M. albus from each group and collected tissues (hepatopancreas, spleen, head kidney and hindgut) for detection of MHC-II-β gene expression using real-time quantitative PCR. MHC-II-β was expressed in four tissues with expression in the head kidney significantly higher (p<0.01) than that in the spleen, hindgut and hepatopancreas. At 20 days, the highest MHC-II-β expression was detected in the 4,000 IU kg-1 group in the head kidney and in the 2,000 IU kg-1 group in the spleen, both of which were significantly higher than that of the other groups (p<0.05). At 40 days, the 1000 IU kg-1 group showed the highest MHC-II-β expression in the head kidney, hindgut and hepatopancreas. At 60 days, the 500 IU kg-1 group showed the highest MHC-II-β expression in the head kidney and spleen and was significant higher than that of the control (p<0.05) while the 4,000 IU kg-1 group showed significantly lower MHC-II-β expression compared to the control (p<0.05). Relative to the MHC-II-β expression in the head kidney, the results demonstrate that short-term (20 days) and high dose dietary provision of VD3 (4,000 IU kg-1) significantly increased MHC-II-β expression in M. albus immune organs. However, the highest MHC-II-β expression was observed with long-term (60 days) dietary provision of VD3 at a dose of 500 IU kg-1.
 
 
 
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