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Articles by Xue Gao
Total Records ( 3 ) for Xue Gao
  Run-Jun Yang , Jun-Ya Li , Xue Gao , Zhi-Hui Zhao , Lu-Pei Zhang , Hui-Jiang Gao and Shang-Zhong Xu
  Fas-Associated Death Domain (FADD) is a signal connection protein in the Fas/FasL system which may play a key role in apoptosis. Follicle atresia and luteolysis are thought to occur by apoptosis. To reveal the intracellular signal transduction molecules involved in the process of follicular development in the bovine ovary. This was accomplished by cloning the FADD gene using RT-PCR, deleting the termination codon in its cDNA and directionally cloning the amplified FADD gene into the eukaryotic expression vector pAcGFP-N1. The pAcGFP-bFADD recombinant plasmid was then transfected into bovine follicular granulosa cells by using lipofectamine 2000. Expression of AcGFP was observed under fluorescent microscopy and the transcription and expression of FADD was detected by RT-PCR and Western-blot. Then the Methyl-Tetrazolium (MTT) assay was performed to determine the growth inhibition of cells. Cell apoptosis was observed by using Hoechst33342 staining and DNA-Ladder Method. The results showed that the pAcGFP-bFADD recombinant plasmid was successfully constructed. AcGFP expression was detected as early as 24 h after transfection. A 654 bp fragment was amplified by RT-PCR and a 51.4 kD target protein was detected by Western-blot. Cells viability decreased significantly at 72 h after transfection and the apoptosis rate of the cells transfected with pAcGFP-FADD was significantly higher than the control group. Cells in the FADD transfection group showed ladder patterns characteristic of apoptosis and the nuclei were shrunken and densely hyperchromatic or fragmented suggesting that FADD is capable of inhibiting the proliferation of bovine follicular granulosa cells and inducing cell apoptosis when over-expressed.
  Li Liu , Zhengrong Yuan , Cui Chen , Limin Zhang , Xiaojie Chen , Xidong Liu , Xue Gao , Junya Li , Huijiang Gao , Lupei Zhang and Shangzhong Xu
  To study the inflammatory response of Peripheral Blood Mononuclear Cells (PBMCs) to actue phases of Staphylococcus aureus (S. aureus) mastitis in dairy cows using gene expression data, the mRNA expression levels of six genes (Iinterleukin (IL)-8, Differentiation (CD) 14, Macrophage Colony Stimulating Factor Receptor (M-CSFR), Toll-Like Receptor 2 (TLR2), Chemokine Receptor 2 (CCR2) and CX3C Chemokine Receptor 1 (CX3CR1) in Peripheral Blood Mononuclear Cells (PBMCs) in infected cows (n = 3) with S. aureus relative to uninfected controls (n = 3) were measured at 0 day before inoculation and 1-3 days post inoculation (dpi) by the real-time quantitative PCR. Results indicated the expression of IL-8, CD14 and CCR2 gene were exhibited significantly (p<0.05) up-regulated within 2 dpi while there was no significant difference in the expression of M-CSFR, TLR2 and CX3CR1 genes at 1-3 dpi. Additionally, concentrations of plasma lipopolysaccharide Binding Protein (LBP) and Somatic Cell Counts (SCCs) were evaluated. Together these data reveal that the upregulation of gene expression is probably related to the recruitment and activation of PBMCs.
  Qianfu Gan , Lupei Zhang , Jiaqin Chen , Haile Yu , Huijiang Gao , Xue Gao and Junya Li
  This study was aimed to search new genetic variants in the bovine A-FABP gene as molecular markers for meat production traits. One SNP (A2989G with) was genotyped by PCR-SSCP Methods. Sequence analysis revealed that SNP was located in exon 2 (A2989G) of A-FABP gene. The gene-specific SNP marker association analysis indicated that the A2989G was significantly associated with marbling score (p<0.05) and loin muscle area (p<0.01). Results from this study suggest that A-FABP gene-specific SNP may be a useful marker for meat quality with traits in future marker assisted selection programs in beef cattle.
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