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Articles by Xu Li
Total Records ( 3 ) for Xu Li
  Xu Li , Eberhard Morgenroth and Lutgarde Raskin
  The potential of a solution-based hybridization assay using peptide nucleic acid (PNA) molecular beacon (MB) probes to quantify 16S rRNA of specific populations in RNA extracts of environmental samples was evaluated by designing PNA MB probes for the genera Dechloromonas and Dechlorosoma. In a kinetic study with 16S rRNA from pure cultures, the hybridization of PNA MB to target 16S rRNA exhibited a higher final hybridization signal and a lower apparent rate constant than the hybridizations to nontarget 16S rRNAs. A concentration of 10 mM NaCl in the hybridization buffer was found to be optimal for maximizing the difference between final hybridization signals from target and nontarget 16S rRNAs. Hybridization temperatures and formamide concentrations in hybridization buffers were optimized to minimize signals from hybridizations of PNA MB to nontarget 16S rRNAs. The detection limit of the PNA MB hybridization assay was determined to be 1.6 nM of 16S rRNA. To establish proof for the application of PNA MB hybridization assays in complex systems, target 16S rRNA from Dechlorosoma suillum was spiked at different levels to RNA isolated from an environmental (bioreactor) sample, and the PNA MB assay enabled effective quantification of the D. suillum RNA in this complex mixture. For another environmental sample, the quantitative results from the PNA MB hybridization assay were compared with those from clone libraries.
  Bin Yang , Xingming Sun , Jianjun Zhang , Lingyun Xiang , Xianyi Chen and Xu Li
  In many critical application areas, such as military, legal and literature fields, methods of protecting copyright for text data could not cause any distortion in textual content. In this study, we evaluate a novel reversible (lossless) data hiding scheme for MS office 2007 document. The proposed scheme modifies the unnoticeable content and the synonyms of a document to hide information. While in extraction processing, the original content can be recovered without any distortion from the marked textual. Our scheme is achieved by using two common text information hiding methods simultaneously. The secret information is embedded into the cover document by synonym substitution. Original synonyms in the document are mapped into a compressed Synonym Index Table (SIT) and concealed into the MS office 2007 document. Experiments results demonstrated that proposed scheme can not only successfully embed and extract the secret information but also keep the original textual content undistorted.
  Yu Yueping and Xu Li
  The study presents the study on the situations of cutting planning of steel plates with the multiple constraints, a mathematical model based on linear programming theory is established. The algorithm has been implemented to optimize the nesting process of large steel plates. The achievements are helpful for the actuality and the higher utilization rate of steel plates of a large-scale steel structure manufacture works.
 
 
 
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