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Articles by Xiushan Wu
Total Records ( 4 ) for Xiushan Wu
  Chuanbin Zhu , Xingzhu Qi , Canding Wang , Wuzhou Yuan , Weiqi Zeng , Yuequn Wang , Zaochu Ying , Yun Den , Mingyao Liu , Yongqing Li and Xiushan Wu
  SVOPs are synaptic vesicle proteins that contain 12 transmembrane regions. Herein, we have isolated a novel human homologue of SVOP gene from a human brain cDNA library. This gene contains an open reading frame of 1646 nucleotides, coding for a protein with 548 amino acids. hSVOP is highly conserved in evolution across different species from C. elegans, fruit fly, mouse, to human. Northern blot analysis showed that it is expressed specifically in human brain. Transient transfection of COS-7 cells with hSVOP-EGFP demonstrates that hSVOP is located on cytoplasmic membrane and may be involved in material transfer between the synaptic vesicle and cytoplasm.
  Weiqi Zeng , Yun Deng , Zhiyuan Yang , Wuzhou Yuan , Wen Huang , Chuanbin Zhu , Yan Bai , Yongqing Li , Zhonglu Peng , Yuequn Wang , Ying Zhu , Mingyao Liu and Xiushan Wu
  The calcium chloride method is an efficient and convenient technique to prepare Escherichia coli competent cells. The transformation efficiency of the competent cells when prepared in the laboratory is generally below 106 cfu μg-1 (colony forming units μg-1) DNA and thus its application is limited in some extent. Here we report the utilization of a-SiNPs (amino modified Silica-Nanoparticles) as a help material to improve the transformation efficiency of E. coli competent cells that are prepared with calcium chloride method. Addition of a-SiNPs increases the transformation efficiency of E. coli to 107-108 from below 106 cfu μg-1 DNA in the laboratory.
  Chuanbing Zhu , Xiangli Ye , Yuejuan Zhang , Hong Yang , Fang Liu , Ying Zhu , Zhenyu Cai , Yuequn Wang , Yun Deng , Wuzhou Yuan , Yongqing Li and Xiushan Wu
  As a model animal for developmental biology, chick embryo is easy to control and observe during embryo development period and therefore it is widely used in the study of cardiac development. The application of proteomics has opened the door for large-scale studies to dissect both protein expression, regulation and function during chick heart developing stages. The proteomics study requires to quickly separate a large number of chick heart samples with the same developing stage. However, the traditional morphological standards based on Hamburger-Hamilton and Witschi stages are difficult to fulfill this requirement. Herein, we suppose a new standard for distinguishing chick heart morphology in different developing stages based on the relationship between the embryonic heart development and the amnion folding in chick. Based on this standard, we can accelerate the speed of embryonic heart sample separation and guarantee the quantity and quality of the sample more reliably.
  Weiqi Zeng , Yuequn Wang , Wuzhou Yuan , Yun Deng , Yongqing Li , Chuanbing Zhu , Mingyao Liu and Xiushan Wu
  We described here a novel domain, CTLH, which can be found in proteins of most already-researched species currently. The domain, consisted of 58 amino acid residues, is not often-conserved in sequence (but has 5 regularly-arranged leucines that are highly conserved). However, its 3D architecture conserves greatly with a typical ŽUŽ shape (alpha helix+beta turn+alpha helix). Although the function of CTLH domain remains unknown, related studies aiming at this domain suggest it participates in the regulation of certain signaling pathways like MAPK, and is involved in some living activities of cells such as protein degradation and mitosis.
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