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| Articles
by
Xiping Fan |
Total Records (
2 ) for
Xiping Fan |
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XiPing Fan
,
QingYan Shang
,
XiaoYyu Zhang
,
JinHuan Han
and
XiaoLin Sun
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To observe ultrastructure of rabbit Cysticercus pisiformis.
Cysticercus pisiformis of naturally infected rabbit was collected and
prepared for Transmission Electron Microscope (TEM) examinations. Under TEM,
Cysticercosis pisiformis appeared to be ovoid form which comprised cyst
wall, cyst fluid, scolex and cervical segment from outer to inner side. The
structure of cyst wall was 3 layers (cortex, mesenchyina and parenchyma) of
which the cortex was composed of 3 layers regular long strip shape cells the
mesenchyina included a large number of glycogen granules and some bunches of
fiber and the parenchyma included two kinds of cells besides glycogen granules
and fiber. One kind was calcareous corpuscles cell and the other was spindle
cell with bigger nuclear. The scolex comprised cortex, mesenchyina and parenchyma
from outer to inner side of which the outside cortex laid a large number of
regular microvilli the mesenchyina laid a large number of structureless fibrous
material and glycogen granules and the parenchyma laid parenchymal cells, cortical
cell, myoblast, flame cell, calcareous corpuscles cell, hamulus, collecting
duct and excretory duct. The structure of cervical segment was similar with
that of the scolex which included collecting duct and gather duct net. |
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Guli Asa
,
Chun Zhang
,
Yonggang Wang
,
Meng He
,
Yilong Yin
,
Xiping Fan
and
Yanming Wei
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A rapid, sensitive and high throughput micro-plate method was established
to quantify polysaccharides in this study. Anthrone-sulfuric acid micro-plate
assay in a certain reaction was tested. A single test and a response surface
methodology was applied to design and optimize four factors including reaction
time, incubation temperature, anthrone concentration and the amount of anthrone
in order to maximize R2 and minimize RSD in each standard curve which
obtained in different conditions. It was demonstrated that the order of significant
impacting R2 is incubation temperature, anthrone concentration, reaction
time and the quantity of anthrone add to while the order of significant impacting
RSD is incubation temperature, anthrone concentration, the amount of anthrone
and reaction time. Preferred reactive condition is be discovered and the best
incubation temperature is 96°C, the best reaction time is 36 min, anthrone
concentration is 1.1 mg mL-1 and the amount of anthrone is 150 μL.
The detectable range of this method is 20-260 μg L-1 under the
optimized condition. Recovery test showed high accuracy and practicability in
this method by recovery rate ranged in 99.5-100.6%. The method was successfully
applied to quantify polysaccharides of different source and the method has high
resistance to interference of protein during measurement process. |
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