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Articles by Xiping Fan
Total Records ( 2 ) for Xiping Fan
  XiPing Fan , QingYan Shang , XiaoYyu Zhang , JinHuan Han and XiaoLin Sun
  To observe ultrastructure of rabbit Cysticercus pisiformis. Cysticercus pisiformis of naturally infected rabbit was collected and prepared for Transmission Electron Microscope (TEM) examinations. Under TEM, Cysticercosis pisiformis appeared to be ovoid form which comprised cyst wall, cyst fluid, scolex and cervical segment from outer to inner side. The structure of cyst wall was 3 layers (cortex, mesenchyina and parenchyma) of which the cortex was composed of 3 layers regular long strip shape cells the mesenchyina included a large number of glycogen granules and some bunches of fiber and the parenchyma included two kinds of cells besides glycogen granules and fiber. One kind was calcareous corpuscles cell and the other was spindle cell with bigger nuclear. The scolex comprised cortex, mesenchyina and parenchyma from outer to inner side of which the outside cortex laid a large number of regular microvilli the mesenchyina laid a large number of structureless fibrous material and glycogen granules and the parenchyma laid parenchymal cells, cortical cell, myoblast, flame cell, calcareous corpuscles cell, hamulus, collecting duct and excretory duct. The structure of cervical segment was similar with that of the scolex which included collecting duct and gather duct net.
  Guli Asa , Chun Zhang , Yonggang Wang , Meng He , Yilong Yin , Xiping Fan and Yanming Wei
  A rapid, sensitive and high throughput micro-plate method was established to quantify polysaccharides in this study. Anthrone-sulfuric acid micro-plate assay in a certain reaction was tested. A single test and a response surface methodology was applied to design and optimize four factors including reaction time, incubation temperature, anthrone concentration and the amount of anthrone in order to maximize R2 and minimize RSD in each standard curve which obtained in different conditions. It was demonstrated that the order of significant impacting R2 is incubation temperature, anthrone concentration, reaction time and the quantity of anthrone add to while the order of significant impacting RSD is incubation temperature, anthrone concentration, the amount of anthrone and reaction time. Preferred reactive condition is be discovered and the best incubation temperature is 96°C, the best reaction time is 36 min, anthrone concentration is 1.1 mg mL-1 and the amount of anthrone is 150 μL. The detectable range of this method is 20-260 μg L-1 under the optimized condition. Recovery test showed high accuracy and practicability in this method by recovery rate ranged in 99.5-100.6%. The method was successfully applied to quantify polysaccharides of different source and the method has high resistance to interference of protein during measurement process.
 
 
 
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