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by
Xiaoyu Guo |
Total Records (
3 ) for
Xiaoyu Guo |
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Jiaohua Qin
,
Xiaoyu Guo
,
XuyuXiang
,
Lingyun Xiang
and
LiliPan
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This study was proposed by a steganalysis algorithm against Multiple Least Significant Bits (MLSB) steganography to estimate the secret message length in images. Firstly, a local masked estimation function by using weighted average of the masked pixels was used to estimate an approximately original image. Then, the weighted stego image was constructed by extending the definition of LSB steganalysis to MLSB one. Finally, the secret message length estimator was constructed through a least square equation. The detailed theoretical proofs of the detection and estimation methods were presented in this study. Experimental results revealed that this method can estimate the length of secret message with high accuracy and low time complexity. |
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Weimin Zuo
,
Xuyu Xiang
,
Yuanyuan Fu
,
Jiaohua Qin
and
Xiaoyu Guo
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The generating test study is a research in constrained multi-object
optimization. It is one of the key technologies in examination management systems.
It relates directly to the efficiency and quality of the generating test paper.
The test paper generation method suggested in this paper is based on hierarchical
adaptive genetic algorithm. It is able to solve the problem of premature convergence
or slow convergence in global optimization. On the one hand, the M subpopulations
operate on adaptive genetic algorithm and save the intermediate result; then
they operate with the top adaptive genetic algorithm until a satisfied paper
is found. On the other hand, the minimum weighted mean square error model is
used to establish the objective function and to inspect the error between the
expectations and the actual value on types, knowledge topics, difficulty and
the degree of differentiation of the test paper. It discusses also the error
of the answer time, total score and luminosity. It improved the speed of generating
test paper of the system. It avoided the problem of premature convergence which
often appears in standard genetic algorithm. The high quality of paper generation
and the good robustness generated in this algorithm can meet the practical needs
of users. |
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Shuqing Liu
,
Xintao Gao
,
Pingjun Li
,
Hong Jia
,
Ting Xin
,
Shaohua Hou
,
Xiaoyu Guo
,
Weifeng Yuan
,
Gaimei Zhang
,
Ming Li
,
Jing Wu
and
Hongfei Zhu
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Bovine tuberculosis caused mainly by Mycobacterium bovis
is a serious zoonosis in the world that can infect cattle and humans as well.
Prevention and eradication of this disease require effective and sensitive diagnosis
method. While the traditional tuberculin skin test and IFN-γ release assay
stimuli as PPD-B may share common antigens with Mycobacterium avium which
could cause false positive results. To overcome this problem, studies are focus
on screening more specific proteins to replace PPD for stimuli recently. Previous
research showed that TB10.4 could activate T cell stimulation therefore, researchers
optimized the expression of TB10.4 by comparing three different recombinant
proteins and using as supplementary antigen stimuli was detected by in diagnosis
of bovine tuberculosis. The pET-32a (+) plasmid and pET-28a (+) plasmid were
chosen which are different in the solubility of the recombinant protein. After
transformation, expression and purification of the recombinants, the recombinant
proteins rTB10.4-1 and rTB10.4-3 were obtained successfully. In order to analysis
the importance of the C terminal for the activity of TB10.4, the initiation
and termination codon were also introduced into the N and C terminal of TB10.4
gene and the rTB10.4-2 lack of His tag protein in the C terminal was obtained.
The result of SDS-PAGE showed that rTB10.4-1, TB10.4-2 and rTB10.4-3 were 30,
30 and 12 ku, respectively which were just the same size as expected. The biological
activities of these recombinant-proteins were compared by IFN-γ release
assay, real-time PCR and Western blot and the results showed that all of the
proteins had good T cell activity and B cell activity. In comparison, rTB10.4-1
possessed strongest activity while rTB10.4-3 was the lowest one as the inclusion
body. The skin test on guinea pigs showed that the DTH in M. bovis-infected
group could be detected intensively by using mixture of recombinant proteins
rTB10.4-1 and rCE in skin test compared to control group. In summary, the recombinant
protein of TB10.4 could be obtained successfully and be used as antigen stimuli
in the skin test which would lay the foundation for manufacture of the diagnosis
kit for bovine tuberculosis with characteristics of high specificity, sensitivity
and reproducibility. |
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