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Articles by Xiaoyu Guo
Total Records ( 3 ) for Xiaoyu Guo
  Jiaohua Qin , Xiaoyu Guo , XuyuXiang , Lingyun Xiang and LiliPan
  This study was proposed by a steganalysis algorithm against Multiple Least Significant Bits (MLSB) steganography to estimate the secret message length in images. Firstly, a local masked estimation function by using weighted average of the masked pixels was used to estimate an approximately original image. Then, the weighted stego image was constructed by extending the definition of LSB steganalysis to MLSB one. Finally, the secret message length estimator was constructed through a least square equation. The detailed theoretical proofs of the detection and estimation methods were presented in this study. Experimental results revealed that this method can estimate the length of secret message with high accuracy and low time complexity.
  Weimin Zuo , Xuyu Xiang , Yuanyuan Fu , Jiaohua Qin and Xiaoyu Guo
  The generating test study is a research in constrained multi-object optimization. It is one of the key technologies in examination management systems. It relates directly to the efficiency and quality of the generating test paper. The test paper generation method suggested in this paper is based on hierarchical adaptive genetic algorithm. It is able to solve the problem of premature convergence or slow convergence in global optimization. On the one hand, the M subpopulations operate on adaptive genetic algorithm and save the intermediate result; then they operate with the top adaptive genetic algorithm until a satisfied paper is found. On the other hand, the minimum weighted mean square error model is used to establish the objective function and to inspect the error between the expectations and the actual value on types, knowledge topics, difficulty and the degree of differentiation of the test paper. It discusses also the error of the answer time, total score and luminosity. It improved the speed of generating test paper of the system. It avoided the problem of premature convergence which often appears in standard genetic algorithm. The high quality of paper generation and the good robustness generated in this algorithm can meet the practical needs of users.
  Shuqing Liu , Xintao Gao , Pingjun Li , Hong Jia , Ting Xin , Shaohua Hou , Xiaoyu Guo , Weifeng Yuan , Gaimei Zhang , Ming Li , Jing Wu and Hongfei Zhu
  Bovine tuberculosis caused mainly by Mycobacterium bovis is a serious zoonosis in the world that can infect cattle and humans as well. Prevention and eradication of this disease require effective and sensitive diagnosis method. While the traditional tuberculin skin test and IFN-γ release assay stimuli as PPD-B may share common antigens with Mycobacterium avium which could cause false positive results. To overcome this problem, studies are focus on screening more specific proteins to replace PPD for stimuli recently. Previous research showed that TB10.4 could activate T cell stimulation therefore, researchers optimized the expression of TB10.4 by comparing three different recombinant proteins and using as supplementary antigen stimuli was detected by in diagnosis of bovine tuberculosis. The pET-32a (+) plasmid and pET-28a (+) plasmid were chosen which are different in the solubility of the recombinant protein. After transformation, expression and purification of the recombinants, the recombinant proteins rTB10.4-1 and rTB10.4-3 were obtained successfully. In order to analysis the importance of the C terminal for the activity of TB10.4, the initiation and termination codon were also introduced into the N and C terminal of TB10.4 gene and the rTB10.4-2 lack of His tag protein in the C terminal was obtained. The result of SDS-PAGE showed that rTB10.4-1, TB10.4-2 and rTB10.4-3 were 30, 30 and 12 ku, respectively which were just the same size as expected. The biological activities of these recombinant-proteins were compared by IFN-γ release assay, real-time PCR and Western blot and the results showed that all of the proteins had good T cell activity and B cell activity. In comparison, rTB10.4-1 possessed strongest activity while rTB10.4-3 was the lowest one as the inclusion body. The skin test on guinea pigs showed that the DTH in M. bovis-infected group could be detected intensively by using mixture of recombinant proteins rTB10.4-1 and rCE in skin test compared to control group. In summary, the recombinant protein of TB10.4 could be obtained successfully and be used as antigen stimuli in the skin test which would lay the foundation for manufacture of the diagnosis kit for bovine tuberculosis with characteristics of high specificity, sensitivity and reproducibility.
 
 
 
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