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Articles by Xiaolin Sun
Total Records ( 2 ) for Xiaolin Sun
  XiPing Fan , QingYan Shang , XiaoYyu Zhang , JinHuan Han and XiaoLin Sun
  To observe ultrastructure of rabbit Cysticercus pisiformis. Cysticercus pisiformis of naturally infected rabbit was collected and prepared for Transmission Electron Microscope (TEM) examinations. Under TEM, Cysticercosis pisiformis appeared to be ovoid form which comprised cyst wall, cyst fluid, scolex and cervical segment from outer to inner side. The structure of cyst wall was 3 layers (cortex, mesenchyina and parenchyma) of which the cortex was composed of 3 layers regular long strip shape cells the mesenchyina included a large number of glycogen granules and some bunches of fiber and the parenchyma included two kinds of cells besides glycogen granules and fiber. One kind was calcareous corpuscles cell and the other was spindle cell with bigger nuclear. The scolex comprised cortex, mesenchyina and parenchyma from outer to inner side of which the outside cortex laid a large number of regular microvilli the mesenchyina laid a large number of structureless fibrous material and glycogen granules and the parenchyma laid parenchymal cells, cortical cell, myoblast, flame cell, calcareous corpuscles cell, hamulus, collecting duct and excretory duct. The structure of cervical segment was similar with that of the scolex which included collecting duct and gather duct net.
  Xilong Kang , Xiaolin Sun , Qiuchun Li , Jing Wang , Zhiming Pan and Xinan Jiao
  Toll-Like Receptor 5 (TLR5), a member of the TLR family, specifically recognizes flagellin which contributes to the motility of bacterial pathogens. Therefore, TLR5 plays a crucial role in host defense against flagellated bacteria. In the current study, researchers cloned the TLR5 gene from Jiangquhai pigs (pTLR5). The pTLR5 open reading frame was fused with a FLAG tag by cloning amplified pTLR5 into the pcDNA3.1-FLAG vector. Flp-In-293 cells expressing pTLR5-FLAG (designated pTLR5-Flp-In-293) were obtained using the Flp recombinase system. β-galactosidase activity and zeocin resistance were lost in the pTLR5-Flp-In-293 cells but hygromycin B resistance was acquired. Reverse Transcription-Polymerase Chain Reaction (RT-PCR) and western blot analysis confirmed that pTLR5-FLAG was expressed in pTLR5-Flp-In-293 cells at both the transcriptional and protein levels. Indirect immunofluorescence assay showed that the pTLR5-FLAG protein was located in the cell membrane of pTLR5-Flp-In-293 cells. Stability analysis by fluorescence-activated cell sorting showed that compared with Flp-In-293 cells, the 1st, 10th and 20th generations of the recombinant cell line expressed consistent levels of pTLR5-FLAG protein. Recombinant pTLR5-Flp-In-293 cells stimulated with flagellin from S. typhimurium expressed high levels of IL-8 which indicated that pTLR5 protein expressed in the pTLR5-Flp-In-293 cell line was a functional TLR5 homolog.
 
 
 
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