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Articles by Xiao-Li Xu
Total Records ( 2 ) for Xiao-Li Xu
  Bing Han , Hui-Ling Zhang , Li Zeng , Bin Yang , Reyanggu Abula , Xiao-Li Xu and Yong Chen
  The beta-adrenergic receptors (β-AR) are a class of metabotropic G protein-coupled receptors that responsible for the muscle growth, fat deposition or lactation characteristics of domestic animals. The aim of this article was to scan nucleotide mutations in partial coding region of β2-AR gene on cattle. In this study, the polymorphisms of β2-AR gene on cattle were detected by methods of Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) and DNA sequencing in two cattle breeds in China, Xinjiang Brown cattle and Chinese Holstein cows. The results revealed that there were two genotypes and homozygote variant was not found in the two breeds. The genotypic frequencies at the Sma I locus were significantly different between the two breeds. The wild-type allele was ascendancy in the two breeds but its allele frequency was distinctly lower in the Chinese Holstein cows than that in the Xinjiang Brown cattle. DNA alignment results showed that three single nucleotide polymorphisms (SNPs), C11A, G53A and C129T were found in 5-coding region of β2-AR gene. The C11A mutation resulted in disappearance of Sma I cleavage site. In addition, the C11A and G53A mutations caused two amino acid residues replacement at the 4th and 18th of β2-AR. However, the C129T mutation was a synonymous one. In conclusion, the heterozygous frequency in Chinese Holstein cows was obviously higher than that in the Xinjiang Brown cattle. The Sma I locus would be a potential genetic marker for lactation performance of cattle.
  Yong Chen , Yong Zhao , Zhu-Yan Fu , Zhao-Wei Ma , Fa-Chen Qian , Anwaier Aibibuli , Bin Yang , Reyanggu Abula , Xiao-Li Xu and Azimaitijiang Aniwaer
  The aim of this study was to investigate nutritive value of tetraploid black locust (Robinia pseudoacacia). Chemical composition and in vitro fermentation characteristics by using in vitro gas production technique were stated for tetraploid black locust, birdsfoot trefoil (Lotus corniculatus L.), alfalfa (Medicago sativa cv.) and galega (Galega orientalis Lam.). Tetraploid black locust showed high crude protein (16.02%), ether extract (2.85%), crude ash (13.16%) and Ca (3.85%) content and low neutral detergent fibre (21.63%), acid detergent fibre (19.91%) and acid detergent lignin (6.52%) content. However, fermentation characteristics, such as cumulative gas production of 48 h, dry matter degradability, neutral detergent fibre digestibility, total volatile fatty acid of tetraploid black locust, were lower than that of birds foot trefoil and alfalfa (p<0.05). Tetraploid black locust fermentation resulted in reducing the molar proportions of acetate and butyrate and increasing that of propionate (p<0.05). In conclusion, according to the chemical composition, tetraploid black locust was a high-quality feed resources. However, utilization of tetraploid black locust by ruminal microorganisms in vitro was limited. Further in vivo studies could be required to expand the knowledge of the nutritive value of tetraploid black locust.
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