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Articles by Xiangchen Li
Total Records ( 3 ) for Xiangchen Li
  Xiangchen Li , Yaxin Yao , Peng Liu , Dapeng Jin , Weijun Guan and M.A. Yuehui
  Bovine oocytes collected from slaughterhouse should be subject to rigorous antiseptic procedures. In this lab, alcohol immersion was applied for preventing microbial infection instead of antibiotics. However, it remains to be investigated whether alcohol treatment would lead to unpredictable results. In the present study, the ovaries were fully washed with 75% alcohol for 1, 5 and 10 min, respectively. Only in the 10 min group, the oocytes apoptotic rates significantly increased (p<0.05) and the development rates significantly decreased (p<0.01) while detectable difference in apoptosis was not observed. Therefore, washing ovaries with 75% alcohol for <5 min constitutes no obvious influences on the development of oocytes and embryos and could be used as a convenient and rapid method to prevent microbial contamination.
  Hui Wang , Xiangchen Li , Changli Li , Wenxiu Zhang , Weijun Guan and Yuehui Ma
  A fibroblast cell line of Qing Kedan chicken was successfully isolated, purified and cryopreseved through direct culturing of explants, serial passage and cryogenic techniques. Assays for biological characteristics were conducted and the results suggested that: the cells cultured with a Population Doubling Time (PDT) of about 20 h, constituted a typical fibroblast cell line in morphology; tests for microbial contamination regarding bacteria, fungi, viruses and mycoplasmas were exclusively negative; Lactate Dehydrogenase (LDH) and Malate Dehydrogenase (MDH) polymorphism analysis disproved the existence of cross-contamination from other cell lines; diploid cells making up 85-93% of the population verified the hereditary stability of the fibroblast line. pEGFP-N3, pEYFP-N1 and pDsRed1-N1 were transfected into the Qing Kedan chicken embryonic fibroblasts with transfection efficiencies between 12.6 and 39.7%. To locate their cellular distribution, transfected cells were observed at 24, 48 and 72 h with confocal microscopy and the fluorescences could be observed throughout the cytoplasm and nuclei of positive cells except in cryptomere vesicles. This research preserved the precious germplasm resource of Qing Kedan chicken at cell level and hence served as an enlightening reference for those of other poultry in the world.
  Peng Cui , Pengfei Hu , Xiangchen Li , Weijun Guan and Yuehui Ma
  A fibroblast line (named SCF36) from 33 Inner Mongolia Cashmere goats ear marginal tissues was established successfully by means of using primary explant technique and cell cryoconservation technology. This fibroblast line contains 336 cryovials with 8x106 cells, respectively. Biological characteristics of the fibroblast line showed that the cells cultured in vitro were all morphologically typical fibroblast and the cell Population Doubling Time (PDT) was approximately 2 days. The cell average viability before freezing was 97.37±2.25 and 91.43±.22% after thawing. Chromosome analysis showed that >90% of the whole population were diploid. Isozyme analysis of Lactic Dehydrogenase (LDH) and Malic Dehydrogenase (MDH) showed that the SCF36 cells had no cross-contamination with other species and the genetic characteristics of the cell line were stable in vitro. Tests for cell line contamination with bacteria, fungi and mycoplasmas were also negative. The transfection efficiency of three fluorescent proteins were relatively high, indicating that the exogenous genes could be effectively expressed in the cells. The cell line met all criteria from the American Type Culture Collection (ATCC). Not only has the germline of this important sheep breed been preserved at the cell level but also valuable material had been provided for genome, postgenome and somatic cloning research and so on.
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