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Articles by X.Y. Zhang
Total Records ( 8 ) for X.Y. Zhang
  A.N. Khan , X.J. Sha and X.Y. Zhang
  This study aims to incorporate location tracking of Mobile Terminals (MTs) for wireless resource management in contrast to existing schemes employing signal strength patterns and signal arrival timing characteristics. The incorporation of location information in Handoff (HO) call handling under heterogeneous traffic conditions can provide better efficiency and Quality of Service (QoS) guaranties. As present results confirm, a HO call handling scheme that takes into account the Received Signal Strength (RSS), speed and call type of users to prioritize HO requests reduces the blocking probability of HO calls, detects false HOs and provides better resource utilization performance as compared to Non Priority and First In First Out HO queuing schemes.
  B.A. Ali , H.H. Salem , X.M. Wang , T.H. Huang , Q.D. Xie and X.Y. Zhang
  The study on vertical transmission of hepatitis B Virus DNA in the human embryo would be an ideal model but such a system presents major logistical, moral and ethical problems. Thus, it is crucial to establish a model system for such study. The present study was designed to amplify HB polymerase gene in one and two cell embryo originated from hamster ova in vitro fertilized with human spermatozoa carrying HBV-DNA. Human sperm carrying HBV-DNA was delivered into zona-free hamster oocytes by in vitro fertilization assay. HB polymerase gene which is responsible for the reverse transcription of the pregenomic RNA to the double stranded DNA has been detected both in one- and two-cell embryos using PCR. The results reveal that HB polymerase gene is present in one- and two-cell embryo (single embryo) originated from hamster ova in vitro fertilized with human spermatozoa carrying HBV DNA sequences. Present results provided the direct evidences that HB-DNA could transmit vertically to next generation via male germ line.
  X.P. Liu , K.W. Chen , K.H. Wang , J.Y. Wang , G.J. Dai , X.Y. Zhang and G.B. Chang
  Xiaoshan chicken is a well-known breed in China. It has good meat quality and three yellow character. SR92A chicken and Xiaoshan chicken are parents. Sixty five offsprings were reared. DNA Fingerprint (DFP) of parental strains were conducted using one middle-repetitive DNA probe EAV (endogenous avian retroviral). Based on presence or absence of band J (3.48 kbp) in their parents, we carry out 4 mating combinations. -/- for absence of band J in both parents; -/+ for absence in sire, presence in dam; +/- for presence in sire, absence in dam; +/+ for presence in both parents. Quantitative traits recorded for offspring are body weight at 75 days and slaughter traits. The results show that the 75 days weight heterosis generated by -/- type is 74.1 g higher than generated by non-selected populations. We can infer that the allele may be corresponding with the different expression of genes between parents and offsprings. We find band J allele has a bearing on weight heterosis, which will lay a solid foundation for locating weight heterosis QTL.
  X.S. Wu , W.B. Bao , J.T. Shu , Q. Xu , X.Y. Zhang , W. Han and G.H. Chen
  This study was designed to investigate the effect of Adenylosuccinate Lyase (ADSL) gene on Inosine Monophosphate content (IMP) in chicken. The chickens used for the study included exogenous Recessive White chicken and Chinese indigenous chicken breeds such as Silkies, Baier, Tibetan and Xiaoshan chickens. A pair of primer was designed to detect exon 2 of ADSL gene by PCR-SSCP and mutation detected was directly sequenced. A synonymous mutation at 3484 bp in exon 2 was substituted C/T SNP. In five chicken breeds, three genotypes TT, CT and CC were observed in exon 2. Muscle IMP content of individuals with TT genotype in exon 2 had significantly higher than those with CT and CC genotypes (p<0.05). In exon 2, Recessive White, Xiaoshan and Baier chickens were in Hardy-Weinberg equilibrium (p>0.05) and Silkies and Tibetan chickens were significant departure from Hardy-Weinberg equilibrium (p<0.01).
  Y.F. Zhu , H.F. Li , W. Han , J.T. Shu , W.T. Song , X.Y. Zhang and K.W. Chen
  The objective of this study was to trace the route of avian Mx (myxo-virus resistance) gene evolution and knowledge of its structure and function variations. Mx gene cDNA sequences of six galliform and anseriform avians were used in this study. Datamonkey and DAMBE were used to detect recombination events and test nucleotide substitution saturation. PAML4b were used to test the selective pressure on amino acid sites. Saturation test did not indicate any sign of substitution saturation. Single breakpoint scanning and genetic algorithm scanning by Datamonkey found two breakpoints, located, respectively in 162 and 999 bp and divided the sequences into three nonrecombination split partitions. The selection test of site-specific mode showed that avian Mx gene sequences had suffered positive selection pressure. Likelihood Ratio Test (LTR) suggested M2a and M8 be more advantageous models,and in a total of eight positive sites with >95% posterior probability were identified in three nonrecombination split partitions. These detected positive sites distributed in N-ternimal and GTP-binding domain of Mx protein and might be important candidate marks for improving avian antiviral activity.
  B.A. Ali , X.M. Wang , G.X. Xu , X.F. Zhao , X.T. Lin , X.Y. Zhang and H.B. Niu
  Quantum Dots (QDs) are autoflorescence semiconductor nanocrystals that can be used for in vivo biomedical imaging. However, we know a little about their in vivo distribution in tissue organs and health consequences. The aim of this study was to detect QDs biodistribution in different organs from healthy female and male mice after single intravenous injection at the dose of 2.98 pmol CdSe/CDs/ZnS QDs/mouse for up to 14 day in female and 8 h in male mice. Laser scanning confocal microscope and/or florescence light microscopy was used to detect QDs in different samples. The results revealed that most of QDs were highly accumulated in spleen, liver, lung of treated mice; however, small amount of QDs was detected in kidney. There is no QDs were observed in other organs such as heart of female mice and brain of male mice of treated group. We also didn’t find QDs in all samples prepared from control group and blood sample of treated mice at different time points. Effective and rapid (1 h) detection of tissue organs and blood samples using fluorescent imaging of quantum dots was demonstrated. This work was done using a very low dose (2.98 pmol/mouse) of injected QDs.
  X.Y. Zhang , M.L. Zhou , X.H. Zhang and D.J. Wu
  To evaluate the genetic polymorphisms and to search for available molecular markers for Liangshan semi-wool sheep, 15 microsatellite markers of 187 samples were amplified by multiplex PCR. A total of 133 alleles were detected, with the number of alleles ranging from 6 (BM2830) to 15 (McM130), giving a mean No. of 8.87 alleles per locus. The total and mean effective allele No. were 64.29 and 4.29, respectively. The observed heterozygosity and expected heterozygosity were from 0.4486 (McM130) to 0.8877 (BMS1678) and 0.5704 (BMS0887) to 0.8373 (McM130), respectively. Mean observed heterozygosity and mean expected heterozygosity were 0.672 and 0.7536. Polymorphism information content values were from 0.5202 (BMS0887) to 0.8183 (McM130) and mean polymorphism information content of the 15 microsatellite loci was 0.7184. All 15 microsatellite loci were highly polymorphic, which showed that there were rich genetic polymorphisms at these detected microsatellite loci in Liangshan semi-wool sheep. Comparison of allele distributions among loci did not reveal consistent shapes. Distributions were centralized in some cases, whereas in others some kind of skewness was evident. Complex evolution at these loci is an important factor in the irregularity of microsatellite distributions.
  X.P. Fan , Q.Y. Shang , X.Y. Zhang , J.H. Han and X.L. Sun
  To observe ultrastructure of rabbit Cysticercus pisiformis. Cysticercus pisiformis of naturally infected rabbit was collected and prepared for Transmission Electron Microscope (TEM) examinations. Under TEM, cysticercosis pisiformis appeared to be ovoid form which comprised cyst wall, cyst fluid, scolex and cervical segment from outer to inner side. The structure of cyst wall was 3 layers (cortex, mesenchyina and parenchyma) of which the cortex was composed of 2 layers regular long strip shape cells, the mesenchyina included a large number of glycogen granules and some bunches of fiber and the parenchyma included two kinds of cells besides glycogen granules and fiber. One kind was calcareous corpuscles cell and the other was spindle cell with bigger nuclear. The scolex comprised cortex, mesenchyina and parenchyma from outer to inner side of which the outside cortex laid a large number of regular microvilli, the mesenchyina laid a large number of structureless fibrous material and glycogen granules and the parenchyma laid parenchymal cells, cortical cell, myoblast, flame cell, calcareous corpuscles cell, hamulus, collecting duct and excretory duct. The structure of cervical segment was similar with that of the scolex which included collecting duct and gather duct net. The ultrastructure of rabbit cysticercosis pisiformis was similar with that of the reported other cestode except a large number of regular microvilli in the cortex of scolex, sparser microvilli in cervical segment than scolex and no microvilli in cyst wall. Furthermore, 2 layers regular long strip shape cells were in the cortex of cyst wall which had no report in cysticercus cellulosae and sheep coenosis.
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