Asian Science Citation Index is committed to provide an authoritative, trusted and significant information by the coverage of the most important and influential journals to meet the needs of the global scientific community.  
ASCI Database
308-Lasani Town,
Sargodha Road,
Faisalabad, Pakistan
Fax: +92-41-8815544
Contact Via Web
Suggest a Journal
 
Articles by X.X. Huang
Total Records ( 3 ) for X.X. Huang
  Y.Z. Tian , J. Di , X.X. Huang , K.C. Tian , W.W. Wu , X.M. Xu , Hanikezi , X.F. Fu , Mayila and Aimaiti
  At present, transcription analysis of gene expression commonly uses a single housekeeping gene as control for normalization. The levels of six housekeeping genes (including 18 SrRNA, GAPDH, ACTB, RPL13A, B2M and TBP) in the skin tissue of fine-wool sheep were estimated by SYBR Green I which belongs to a method of real-time fluorescence quantitative RT-PCR expression. The results showed that differences in expression levels were observed by analysis of geNorm program, an optimal number of control genes for normalization in skin tissue of fine-wool sheep would be 3 and 18 SrRNA, GAPDH, ACTB were finally determined as suitable internal control genes with the results of V2/3(0.163)>0.15, while V3/4(0.100)<0.15 and the M value B2M>RPL13A>TBP> 18 SrRNA>ACTB(GAPDH). The results of this study revealed using three genes (ACTB, GAPDH and 18 SrRNA) in experimental system about sheep, their mRNA expression levels would not change apparently were found. The significance of this study provided convincing references and methodology for selection of housekeeping genes and normalization in gene expression analysis with RT-PCR. In conclusion, the necessity of choosing reference genes was proved and a good way was introduced to select reference genes when experiments were handled by different empirical factors (especially under the effect of new materials). This approach might be useful for studying on the candidate genes which associated with the wool fineness.
  X. Yu , X.X. Huang , W.J. Liu , Y. Fang , L. Shi , W.T. Xing , C.W. Tang and M.A. Brown
  The genetic variability and genetic relationship of the fifteen sheep breeds were studied. The genotypes of 14 indigenous Chinese sheep breeds and one crossbreed of wild sheep were investigated using 13 microsatellite DNA markers recommended by the Food and Agriculture Organization of the United Nations (FAO) and the International Society of Animal Genetics (ISAG) through PCR. The allele frequency, heterozygosity and Genetic Differentiation Index (Fst) were computed to estimate the genetic variation of each population. To determine the genetic relationships among the breeds, phylogenetic trees were constructed based on Nei’s genetic distance using the unweighted pair-group method with arithmetic mean and neighbor-joining method. A total of 15 breeds were clustered into three groups. The first group included Altay sheep and Duolang sheep, the second group was very large and complicated, the third group was a Kazark sheep. The thirteen microsatellite loci were effective markers for the analysis of genetic relationship among sheep breeds. In addition, genetic distance among groups is not according with their geographic distribution and groups or breeds with low production performance can easily impacted by breeds with better production performance. As for the results, it may be result from applying on artificial insemination and extension of commercial cross breeding technique in sheep production system recent years in China.
  J.L. Gao , X.X. Huang , H.T. Song , W. Chen and N.H. Quan
  To realize the high-speed, realtime and high-reliability communication between the controller and the servo motors, the high-speed communication system is developed. It is based on Sercos II (serial real time communication specification II). One modeling scheme fitting for the communication system based on Object-Oriented Technology (OOT) and Unified Modeling Language (UML) is presented. It gives in-depth discussion about modeling the system based on UML, designing of the software and hardware of Sercos II master/slave station, real-time performance calculation and consistency verification. Eventually, the system is applied in 3-axis numerical control machine tool. It can send the control instructions with 4 bytes to 1-6 servo motion axles and receive the feedback parameters with 4bytes within control cycle of 0.5 m sec.
 
 
 
Copyright   |   Desclaimer   |    Privacy Policy   |   Browsers   |   Accessibility