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Articles by X.S. Wu
Total Records ( 3 ) for X.S. Wu
  B.C. Li , G.H. Chen , J. Qin , X.S. Wu , S.L. Wu and Z.T. Cai
  The aim of this study was to evaluate the efficiency of isolation and culture of PGCs from various tissues of chicken embryos at specific developmental stages including: the circulating blood of stage 14 embryos (hatched for 48-52hrs), the genital ridge of stage 19 embryos (hatched for 68-72hrs) and the gonad of stage 28 embryos (hatched for 128-132hrs). Ficoll density-gradient centrifugation is a standard method for the purification of PGCs from fetal blood. The genital ridge and gonadal tissue contain more PGCs in total but must first be digested with trypsin-EDTA to give a single cell suspension containing a mixture of PGCs and other contaminating cell types. In these experiments, we cultured PGCs from the genital ridge and from gonadal tissue before and after Ficoll density-gradient purification. In all cases, PGCs were subsequently cultured in TCM-199 medium supplemented with 10% fetal calf serum. The results demonstrated that trypsin-EDTA alone of the genital ridge of stage 19 embryos yielded a total of2.7 x 104 per embryo of which 89.5% were viable. After Ficoll density-gradient purification of these cells the yield was 1.8 x 104 of which 87.5% were viable. Processing of the gonadal tissue of stage 28 embryos yielded a total of 3.1 x 104 PGCs per embryo of which 90.0% were viable. It was clear that the PGC yield with trypsin-EDTA alone was higher (P< 0.01) than the yield of the full procedure which included the Ficoll density-gradient purification step. The results of PGC culture from the three developmental stages indicated that the survival time was longest (80-88 hours) for PGCs obtained from stage 19 embryos. At this stage, a large number of PGCs had accumulated in the genital ridge which facilitated the isolation procedure. These results suggest that the highest yield of PGCs per embryo can be achieved by trypsin-EDTA treatment of genital ridge tissue from stage 19 chicken embryos.
  H.H. Musa , J.H. Cheng , X.S. Wu , H.P. Ju , D.M. Mekki and G.H. Chen
  Present study was focus to compare LDL receptor mRNA expression, total cholesterol, triglyceride, lipoprotien levels and abdominal fat weight in genetically fat and lean chickens. Genetically lean (Rugao) and fat (Anka) chickens were reared in the same environmental condition, at 12 weeks of age samples of liver tissue were collected and abdominal fat weight was determined. Similarly, total cholesterol, triglycerides and high density lipoprotein were assayed using a commercial enzymatic kit, very low density lipoprotein and low density lipoprotein were estimated using the Friedewald equation. Total RNA from liver tissues were isolated using the standard Trizol methods and then total RNA was reverse transcribed by moloney murine Leukemia virus. Semi-quantitative RT-PCR was developed to quantify the levels of LDL receptor mRNA expression. The level of LDL receptor expression was significantly (p< 0.05) difference between lean and fat chicken. In addition, lean and fat chickens were significantly differed on triglyceride, very low density lipoprotein and abdominal fat weight. The expression of LDL receptor mRNA in liver of fat chicken was negatively correlated with abdominal fat weight. However, in lean chicken was negatively correlated with total cholesterol, triglyceride, lipoprotein concentration, abdominal fat weight and percentage of abdominal fat weight. In addition, within two breeds LDL receptor mRNA expression in liver was negatively correlated with low density lipoprotein, abdominal fat weight and percentage of abdominal fat weight.
  Musa H.H. , G.H. Chen , W.B. Bao , X.S. Wu and J.H. Cheng
  Meat quality such as tenderness, color Density (OD), pH and Water Holding Capacity (WHC) were estimated from breast muscle of genetically fat and lean chickens at 12 week of age. Mutation in lipoprotein lipase and apoVLDL-II genes was detected by PCR-SSCP techniques. Agreement of the genotype frequencies with the Hardy-Weinberg equilibrium expectations was tested using a chi-square goodness of -fit test. Lipoprotein lipase gene frequency was significantly different (p<0.01) in Rugao population, whereas in apoVLDL-II gene there are no significantly different between populations. The populations were differed significantly (p<0.01) within two genes. Lipoprotein lipase genotype was significantly (p<0.05) effect water holding capacity and meat tenderness. However, apoVLDL-II genotype was non significantly affected meat quality traits. The results also indicated that the interaction of Lipoprotein lipase and apoVLDL-II genotype was significantly (p<0.01) effected color density, pH and meat tenderness, whereas it was non significantly effects water holding capacity of breast muscle.
 
 
 
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