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Articles by X.M. Wang
Total Records ( 2 ) for X.M. Wang
  B.A. Ali , H.H. Salem , X.M. Wang , T.H. Huang , Q.D. Xie and X.Y. Zhang
  The study on vertical transmission of hepatitis B Virus DNA in the human embryo would be an ideal model but such a system presents major logistical, moral and ethical problems. Thus, it is crucial to establish a model system for such study. The present study was designed to amplify HB polymerase gene in one and two cell embryo originated from hamster ova in vitro fertilized with human spermatozoa carrying HBV-DNA. Human sperm carrying HBV-DNA was delivered into zona-free hamster oocytes by in vitro fertilization assay. HB polymerase gene which is responsible for the reverse transcription of the pregenomic RNA to the double stranded DNA has been detected both in one- and two-cell embryos using PCR. The results reveal that HB polymerase gene is present in one- and two-cell embryo (single embryo) originated from hamster ova in vitro fertilized with human spermatozoa carrying HBV DNA sequences. Present results provided the direct evidences that HB-DNA could transmit vertically to next generation via male germ line.
  B.A. Ali , X.M. Wang , G.X. Xu , X.F. Zhao , X.T. Lin , X.Y. Zhang and H.B. Niu
  Quantum Dots (QDs) are autoflorescence semiconductor nanocrystals that can be used for in vivo biomedical imaging. However, we know a little about their in vivo distribution in tissue organs and health consequences. The aim of this study was to detect QDs biodistribution in different organs from healthy female and male mice after single intravenous injection at the dose of 2.98 pmol CdSe/CDs/ZnS QDs/mouse for up to 14 day in female and 8 h in male mice. Laser scanning confocal microscope and/or florescence light microscopy was used to detect QDs in different samples. The results revealed that most of QDs were highly accumulated in spleen, liver, lung of treated mice; however, small amount of QDs was detected in kidney. There is no QDs were observed in other organs such as heart of female mice and brain of male mice of treated group. We also didn’t find QDs in all samples prepared from control group and blood sample of treated mice at different time points. Effective and rapid (1 h) detection of tissue organs and blood samples using fluorescent imaging of quantum dots was demonstrated. This work was done using a very low dose (2.98 pmol/mouse) of injected QDs.
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