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Articles by X.H. He
Total Records ( 4 ) for X.H. He
  X.C. Li , P.F. Hu , T.F. Lu , X.H. He , W.J. Guan and Y.H. Ma
  Tan sheep is one of the most distinctive local sheep breeds in China, while investigations concerning its cell biology and molecular genetics are still scarce. To preserve the valuable genetic resources of Tan sheep, we established a Tan sheep fibroblast line and identified its biological characteristics, including cell morphology, growth curve, karyotype, isoenzyme polymorphism and expression of exogenous fluorescent genes. The results showed that the viability of the fibroblasts before freezing was 96.75±3.24 and 93.42±2.87% after thawing. The growth curve of Tan sheep ear marginal fibroblasts displayed an obvious “S” shape and there was no microbial contamination and cross-contamination; all somatic chromosomes were acrocentric autosomes and only the two sex chromosomes were submetacentric; the transfection efficiencies of the three fluorescent protein genes were 20.6-36.2%. It could be concluded that the this study has not only provided the biological characteristics of Tan sheep at cellular level but also made a valuable contribution to the preservation of the genetic resources of the Tan sheep.
  M.J. Xu , Y. He , R. Liang , D.H. Zhou , R.Q. Lin , C.C. Yin , X.H. He , M. Liang and X.Q. Zhu
  The present investigation aimed to examine the prevalence of Chlamydia infection in pigs in Guangdong Province, Southern China. Between March 2008 and May 2009, a total of 1,017 serum samples were collected from 15 intensive farms, which are distributed in 12 administrative cities of Guangdong Province and assayed for Chlamydia antibodies by Indirect Hemagglutination Assay (IHA). The results showed that almost all of the investigated farms showed seroprevalence (93.33%, 14/15). The average seroprevalence of pigs with Chlamydia antibodies was 30.78% (ranged between 0-88%) with breeding boars having the highest prevalence (63.38%, 45/71), which was followed by breeding sows (41.10%, 60/146) and fattening pigs (36.25%, 29/80). These results indicated that prevalence of Chlamydia infection in pigs from intensive farms in Southern China is high, therefore integrated and improved control strategies and measures should be implemented to prevent and control Chlamydia infection in pigs.
  G.H. Peng , Z.G. Yuan , D.H. Zhou , X.H. He , C. Yan , C.C. Yin , Y. He , R.Q. Lin , H.Q. Song and X.Q. Zhu
  Toxoplasma gondii infects nearly one third of the total population of the world, as well as warm blooded animals causing serious public health problems and economic losses in the world. Micronemes plays a key role in the invasion process of T. gondii which are used for host cell recognition, binding and motility. In this research, the researchers examined sequence variation in the microneme protein 4 (MIC4) gene sequences of 12 T. gondii isolates and reference strains from different hosts and geographical locations, then constructed the DNA vaccine expressing MIC 4 of T. gondii evaluated its immune response induced in Kunming mice. The results demonstrated that sequence variation in MIC 4 among different T. gondii isolates was low, which is a useful feature as a vaccine candidate. Immunization of mice with pVAX-MIC4 induced strong immune responses in mice as shown by significant lymphocyte proliferation, cytokine production and antibody responses as well as increased survival time of the immunized mice after challenge with tachyzoites of the virulent T. gondii RH strain, demonstrating that T. gondii MIC4 is a potential vaccine candidate against toxoplasmosis.
  Y.Z. Cheng , J. Hou , X.H. He , Z.K. Hong , L.S. Li , G.H. Lin , M.X. Chen and S.H. Chen
  Paragonimiasis and angiostrongyliasis are important food-borne parasitic diseases in a number of countries including China. Both diseases are transmitted by freshwater and terrestrial intermediate snail hosts. In the present investigation, we examined the prevalence of Paragonimus and Angiostrongylus cantonensis in the intermediate host snails in southeastern China’s Fujian province where paragonimiasis and angiostrongyliasis are endemic. The prevalence of P. westermani cercariae in the stream-type snail Semisulcospira libertine found in the breeding grounds in Shouning county was 0.09% in the upstream and 0.56% in the downstream, respectively. For pit-ditch type, the prevalence of P. westermani cercariae was 0.19% and 0.82% in upstream and downstream, respectively. The snail Tricula xiaoqiaoensis was infected with Paragonimus skrjabini cercariae with prevalence of 0.33% in the pit-ditch type and 1.52% in the seepage type in Zhenghe county. Prevalence of A. cantonensis larvae in Achatina fulica in Nan’an county in garbage stack, vegetable plot and crop land were 83.54, 39.53 and 19.23%, respectively. In Lianjiang county, prevalence of A. cantonensis larvae in snails Pila polita in trench, paddy field, residential ditch, vegetable irrigation ditch and pond in the same river system were 4.08, 8.82, 75.34, 34.04 and 5.56%, respectively. The snail Bellamya lithophaga found in the western suburb of Fuzhou city was identified as the new intermediated host for A. cantonensis with a prevalence of 14.38%. Demonstration of prevalence of Paragonimus and A. cantonensis in wild snails in Fujian province poses substantial risk for future outbreaks of the two food-borne parasitic diseases.
 
 
 
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