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Articles by Wolfgang Nellen
Total Records ( 4 ) for Wolfgang Nellen
  Jamsari , Lily Syukriani , Hashlin Pascananda Utami , Friedrich Herberg , Wolfgang Nellen and Istino Ferita
  Artificial inoculation is an important method for understanding the plant-virus interaction. Currently available techniques for virus artificial infection are whitefly based inoculation, grafting, mechanical, agroinoculation and particle bombardment. In fact, almost all the available inoculation methods do not result in ideal efficiency nor practicality. The purpose of this study is to establish an injection based technique for routine artificial inoculation of Geminivirus particle into chilli pepper plant. The following treatments were applied in this study: Different ratios of extract-buffer (0, 20 and 60% w/v) and injection at four different positions (no inoculation, shoot, middle of main vein and petiol). The presence of Geminivirus particles in plants whether for inoculum source and infection effectivity was confirmed using the polymerase chain reaction. Some morphological observations were also applied in measurement of infection effectivity. Overall, the incidence of infection was 18% and among these, the average intensity of disease was 53% in the four week post inoculation. Geminivirus transmission by injection technique described in this study could be a simple and effective method for geminivirus artificial infection. However, accuracy, simplicity and uniformity have to be accommodated for further application of this technique.
  Jamsari , Istino Ferita , Lily Syukriani , Helni Lalan , Friedrich Herberg and Wolfgang Nellen
  Whiteflies have attracted intensive attention from many agriculturists due to their direct feeding and plant virus transmission. The economic losses depend on certain species population existing on the field during chilli-pepper cultivation. More over, biotype characteristic will determine the effectivity of applied pest management. Based on this, detailed information on whiteflies in chilli-pepper cultivation is very important. We applied mitochondria cytochrome oxidase unit I gene sequence for detecting species and biotype of whiteflies existing in West Sumatera. Two species were successfully identified i.e., Bemisia tabaci and Trialeourides vaporariorum that are dominant in this region. Distribution of both species are separated by altitude, where B. tabaci exist predominantly from low altitude to medium altitude, whereas in high altitude it is not common. On the other hand, T. vaporariorum distribute mainly in high altitude and not in medium altitude. Furthermore biotype determination on B. tabaci identified the existence of B biotype in all areas studied.
  Hui Li , Qian Chen , Markus Kaller , Wolfgang Nellen , Ralph Graf and Arturo De Lozanne
  Aurora kinases are highly conserved proteins with important roles in mitosis. Metazoans contain two kinases, Aurora A and B, which contribute distinct functions at the spindle poles and the equatorial region respectively. It is not currently known whether the specialized functions of the two kinases arose after their duplication in animal cells or were already present in their ancestral kinase. We show that Dictyostelium discoideum contains a single Aurora kinase, DdAurora, that displays characteristics of both Aurora A and B. Like Aurora A, DdAurora has an extended N-terminal domain with an A-box sequence and localizes at the spindle poles during early mitosis. Like Aurora B, DdAurora binds to its partner DdINCENP and localizes on centromeres at metaphase, the central spindle during anaphase, and the cleavage furrow at the end of cytokinesis. DdAurora also has several unusual properties. DdAurora remains associated with centromeres in anaphase, and this association does not require an interaction with DdINCENP. DdAurora then localizes at the cleavage furrow, but only at the end of cytokinesis. This localization is dependent on DdINCENP and the motor proteins Kif12 and myosin II. Thus, DdAurora may represent the ancestral kinase that gave rise to the different Aurora kinases in animals and also those in other organisms.
  Jamsari Jamsari , Istino Ferita , Ade Noverta , Eko Dharma Husada , Friedrich W. Herberg , Wolfgang Nellen and Lily Syukriani
  Background and Objective: In order to elucidate pathogenic determining factor of pepper yellow leaf curl virus isolated from a lowland chili pepper cultivation field in West Sumatra, Indonesia, a whole genome analysis via primer walking strategy was conducted. Materials and Methods: The whole DNA A-like genome sequence of PepYLCIpsV was elucidated via five steps of primer walking, started with universal primers PAR1c715 and PAL1v1978 as the start point. Results: Whole genome comparison analysis identified only one single base insertion/deletion event located in the common region from a previously described isolate collected from the upland location (PepYLCItdV). The BLAST comparison on the nucleotide level showed 90.6% maximal homology with the existing DNA A-like genome from many pepper yellow leaf curl viruses deposited in public database so far. Further detail comparison each of six Open Reading Frames (ORFs) between PepYLCIpsV and PpepYLCItdV indicated that V1 and V2 displayed 94-95% homology, respectively, while C2 and C3 had homology in range of 99 and 97%, respectively. Interestingly C1 and C4 showed homology only 79 and 68%, respectively and the Common Region (CR) shared only 74% similarity. Conclusion: The C1 and CR could be the determining factors for the pathogenicity, therefore, characterizing of these two regions in the population could be used for management and controlling of the virus. The pathogenic isolate PepYLCIpsV (KT809345) appears to be derived by recombination from two isolates originating from different regions and hosts. Its existence seems to be more ancient than its currently mild dominant counterpart PepYLCItdV (KT809346).
 
 
 
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