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Articles by Wizna
Total Records ( 10 ) for Wizna
  R. F. Ramadhan , Wizna , Yetti Marlida and Mirzah
  Objective: The aim of this study was to find out the best combination of bovine blood and agri-industrial waste which fermented by Bacillus amyloliquefaciens with different fermentation time to increase the quality of blood mixtures such as protein content, fiber content, nitrogen retention, amino acid composition and enzyme activity. Methodology: Bovine blood and agri-industrial waste obtained from a slaughterhouse and traditional markets. The design used in this study was a Completely Randomized Design (CRD) factorial using three factors. Results: The results showed that there was a highly significant interaction (p<0.01) between blood concentrations, types of agricultural waste and fermentation time on crude protein, nitrogen retention and energy metabolism which the best results was mixtures blood (300/250 mL) and coconut pulp with fermentation time 120 h whereas, the crude protein content, nitrogen retention and enzyme activity (protease and mannanase) were 50.70 and 55.60%, protease 37.34 U g–1 and mannanase 0.992 U g–1, respectively. Meanwhile, the mixtures of blood (300/250 mL) and PKC had the best amino acid composition. Conclusion: The study can be concluded that the best results were mixture of blood meal (300 mL) with coconut pulp and fermentation time 120 h.
  Wizna , Yose Rizal , Hafil Abbas , Abdi Dharma and I.P. Kompiang
  An experiment was conducted to determine the effects of substitution of Bacillus amyloliquefaciens fermented tapioca by-products for some of corn meal in the diets on the performance of broilers and ducklings. 200 unsexed day old broiler chicks and 200 male day old ducklings were randomly allocated into 40 pens (teen chicks or duckling/pen). This experiment was arranged in a completely randomized design with five dietary treatments (0, 10,20, 30 and 40% for broilers and 0, 30, 40, 50 and 60% for ducklings) of fermented by-product tapioca in diets and four replications. Measured variables were those of feed consumption, average body weight gain, feed conversion and carcass percentage. Results of experiment indicated that feed consumption, average body weight gain, feed conversion and carcass percentage were not affected (p>0.05) by levels of Bacillus amyloliquefaciens fermented tapioca by-products in the diets for broilers. However average body weight gain was affected (p<0.05) and feed conversion were highly affected (p<0.01) by levels of Bacillus amyloliquefaciens fermented tapioca by-products in the diets for ducklings. Increasing levels of Bacillus amyloliquefaciens by products of tapioca up to 60% in diets of ducklings increased their body weight gain and reduced their feed conversion. In conclusion, Bacillus amyloliquefaciens- fermented tapioca by-products can be included up to 40% in diets (replace 67% corn meal) for broilers and up to 60% in diets for ducklings (replace 100% corn meal).
  Zurmiati , Wizna , M. Hafil Abbas , Maria Endo Mahata and Revan Fauzano
  Background and Objective: Live probiotic feed additives can balance microorganism populations in the digestive tract. This study aimed to investigate the effects of the probiotic Bacillus amyloliquefaciens on growth performance and feed efficiency of Pitalah starter ducks. Methodology: A completely randomized design was used in this study. Three different doses of B. amyloliquefaciens (1000, 2000 and 3000 ppm) were dispensed in the drinking water of 1 day old Pitalah ducks (N = 25 duck per dose) and each treatment was replicated five times, control birds were given 0 ppm (N = 25 duck). Feed consumption and conversion, body weight gain, income over feed cost, total colony count of Bacillus sp., in the small intestines and acidity in small intestine were measured. Results: The results showed that all three doses significantly decreased feed consumption, feed conversion and small intestinal acidity (p<0.05) and increased income over feed cost and total small intestinal Bacillus sp., colony counts. There was non significant change in weight gain (p>0.05). Conclusion: It is concluded that addition of 2000-3000 ppm of B. amyloliquefaciens to the drinking water of Pitalah ducks can improve the feed efficiency by >15% and provide economic benefits for farmers raising starter ducks.
  R.F. Ramadhan , C. Yang , Wizna , Y. Marlida and Mirzah
  Background and Background: Bovine blood is a livestock by-product that can be used as a protein source for livestock, particularly when incorporated in broiler rations. This study was conducted to investigate the effects of amino acids in Bacillus amyloliquefaciens-fermented mixtures of bovine blood and coconut pulp (blood meal) on growth performance, blood cholesterol and erythrocyte content in broilers. Methodology: A total of 100 six day-old CP 707 (Strain Cobb) broilers were divided into 5 experimental groups with 4 replicates of 5 broilers. The experimental groups received rations supplemented with 0, 5, 10, 15 and 20% blood meal that substituted for fish meal or soybean meal. The experimental period was 5 weeks. Treatment effects on broiler performance parameters such as final body weight, final weight gain, average daily gain, feed intake, average abdominal fat and Feed Conversion Ratio (FCR), total serum cholesterol and erythrocyte count, as well as the return on investment were determined. Results: The group fed rations supplemented with 10% blood meal showed final body weight, final weight gain and FCR of 1,172, 1,035 and 1.75 g, respectively, which was significantly higher (p<0.05) than that for the other treatments. Rations with 10% blood meal also had the best return on investment ($0.92) relative to the other groups. Conclusion: A mixture of 10% bovine blood and coconut pulp fermented with Bacillus amyloliquefaciens can be used in broiler feed without adversely affecting broiler performance and can replace 47% of total fishmeal and 53% of soybean meal in broiler rations.
  Zurmiati , Wizna , M. Hafil Abbas and Maria Endo Mahata
  Background and Objective: The Pitalah duck is one of Indonesia’s native ducks that has a uniform physical form and a genetic composition that is well adapted to environmental conditions. However, the Pitalah duck has a high feed conversion, so it is necessary to improve its feed efficiency. The purpose of this study was to determine the effect of the balance of energy and protein in the diet, which includes a probiotic, Bacillus amyloliquefaciens (B. amyloliquefaciens), on the performance of the Pitalah duck. Methodology In this study a 3×3 group-randomized factorial design was employed that included 2 factors with 3 groups of body weight as replicates. Factor A is the energy level (E1: 2800, E2: 2700 and E3: 2600 kcal kg–1) and factor B is the protein level (P1: 18, P2: 17 and P3: 16%). Live weight, percentage of carcass, percentage of abdominal fat and income over feed cost were the measured variables. Results: The results showed that the combination of energy level and protein level and an interaction between these factors had a significant impact (p<0.05) on the live weight, percentage of carcass, percentage of abdominal fat and increased income over feed cost. Conclusion: The combination of energy and protein, along with administering 2000 ppm of the probiotic B. amyloliquefaciens, can improve the efficiency of the ration while decreasing the need for duck ration energy by 3.57% and decreasing the protein requirement by 5.56% at an energy level of 2700 kcal kg–1 and 17% protein in the ration.
  Ulvi Fitri Handayani , Wizna , Irfan Suliansyah , Yose Rizal and Maria Endo Mahata
  Objective: An experiment had been conducted to evaluate the nutrient content of tomato waste for laying hen feed after treating tomato waste with different heating methods. Materials and Methods: The tomato waste used in this experiment was comprised of rejected fresh tomatoes from traditional markets. An experiment was performed in a 2×5 factorial arrangement using a completely randomized design (CRD) with 3 replicates. The first factor consisted of two different heating methods (steaming and boiling) and the second factor consisted of five heating durations (0, 4, 8, 12 and 16 min). The measured variables were lycopene (mg/100 g), dry matter (%), organic matter (%), ash (%) and nutrient content of tomato waste [crude protein (%) and crude fiber (%)]. Results: There was no interaction (p>0.05) between heating method and heating duration for lycopene, dry matter, crude protein, crude fiber, organic matter or ash content, while heating method significantly affected (p<0.05) organic matter and ash content. The heating duration also significantly affected (p<0.05) lycopene, dry matter, crude protein, crude fiber, organic matter and ash content of tomato waste. Boiling was better than steaming for organic matter content, while steaming was better than boiling for ash content. Crude protein and crude fiber in boiling and steaming treatments were not different. The heating duration also significantly affected (p<0.05) lycopene, dry matter, crude protein, crude fiber, organic matter and ash content of tomato waste. A heating duration of 12 min increased lycopene and organic matter content and maintained the crude protein content. Conclusion: Steaming was the appropriate method for heating tomato waste based on ash content. Heating tomato waste for 12 min was the appropriate method for increasing lycopene and organic matter content and maintaining the crude protein content.
  Wizna , Hafil Abbas , Yose Rizal , Abdi Dharma and I. Putu Kompiang
  An experiment was conducted to improve the nutrient content of sago pith (Metroxylon sago Rottb) and rumen content mixture through fermentation by using cellulolytic bacteria (Bacillus amyloliquefaciens) as inoculums. The experiment was determination of the optimum conditions (dosage of inoculums, fermentation length and temperature) for sago pith and rumen content mixture fermentation based on nutrient quality and quantity of these fermented products. The study was conducted in experimental methods, using the completely randomize design in factorial with 3 treatment were : 1. A factor (Dosage of inoculums: A1 = 2%, A2 = 6%, A3 = !0%), 2. B factor (Fermentation length : B1 = 3 days, B2 = 6 days, B3 = 9 days) and 3. C factor (Temperature : C1 = 30oC, C2 = 40oC and C3 = 50oC). Results of study showed that optimum conditions of the fermentation of sago pith (Metroxylon sago Rottb) and rumen content mixture was at 2% dosage of inoculums, 9 days of fermentation length and 40oC temperature. This conditions can decrease 33% of crude fiber and increase 42% of crude protein which made the nutritional value of the product based on dry-substance was 15.79% crude protein, 2.75% crude fat, 18.54% crude fiber, 0.20% calcium, 0.16% phosphor, 2540 Kcal/kg metabolic energy, and 66.65% nitrogen retention.
  Wizna , Hafil Abbas , Yose Rizal , Abdi Dharma and I. Putu Kompiang
  An experiment was conducted to improve the nutrient content of tapioca by-products (onggok) through fermentation by using cellulolytic bacteria (Bacillus amyloliquefaciens) as inoculums. The experiment was determination of the optimum conditions (dosage of inoculums, fermentation length and temperature) for tapioca by-products (onggok) fermentation based on nutrient quality and quantity of these fermented products. The study was conducted in experimental methods, using the completely randomize design in factorial with 3 treatment were: 1) A factor (Dosage of inoculums: A1 = 2%, A2 = 6%, A3 = !0%), 2) B factor (Fermentation length: B1 = 3 days, B2 = 6 days, B3 = 9 days) and 3) C factor (Temperature: C1 = 30oC, C2 = 40oC, C3 = 50oC). Results of study showed that optimum conditions of the fermentation of tapioca by-products (onggok) was at 2% dosage of inoculums, 6 days of fermentation length and 40oC temperature. This conditions can decrease 32% of crude fiber and increase 360% of crude protein which made the nutritional value of the product based on dry-substance was 7.9% crude protein, 2.75% crude fat, 11.55% crude fiber, 0.26% calcium, 0.17% phosphor, 2190 Kcal/kg metabolic energy and 65.95% nitrogen retention.
  Wizna , Hafil Abbas , Yose Rizal , Ade Djulardi and Helmi Muis
  An experiment was conducted to improve the nutrient content of rice bran which supplemented with Zn, Sulfur and urea through fermentation by using cellulolytic bacteria (Bacillus amyloliquefaciens) as inoculums. The objective of this experiment was to study the effect of supplementation of catalytic substrate consisting of Zn, sulfur and urea on the substrata characteristics and its microbial population.The experiment was determination of the optimum conditions (dosage of Zn, Sulfur and urea) for rice bran fermentation based on nutrient quality and quantity of these fermented products. The study was conducted in experimental methods, using the completely randomize design in factorial with 3 treatments were : 1) A factor (Dose of urea: A1 = 1.0%, A2 = 1.5%, A3 = 2.0%), 2) B factor (Dose sulfur: B1 = 0.2%, B2 = 0.4%, B3 = 0.8%) and 3) C factor (Dose Zn: C1 = 0.0025%, C2 = 0.005%, C3 = 0.0075%). Results of study showed that optimum conditions of the fermentation of rice brand was at 2% urea, 0.0025% Zn and 0.2% sulfur. The protein level increased 100% and the mineral content 60%, accompanied by 8.2% of increase in the digestibility. The level of phytic acid decreased 97%. This conditions can increase 36% of metabolizable energy, 15% nitrogen retention, 98% crude fiber digestible.
  Zurmiati , Wizna , M. Hafil Abbas and Maria Endo Mahata
  Background and Objective: Mannanase is a decomposer of the compound mannan, which is produced by many microorganisms including bacteria. This enzyme can be utilized in various industrial processes, such as in the animal feed industry to increase the nutritional value of the feed. This study aims to optimize the production and function of the mannanase enzyme, which is produced by Bacillus amyloliquefaciens on a coconut waste substrate. Methodology: This study was designed in the form of laboratory experiments described herein and repeated 3 times. The substrate dose, incubation time, optimum pH and temperature of the mannanase enzyme were measured. Results: The results of this study showed that the best conditions were a substrate ratio of 80% coconut waste to 20% tofu waste, a 48 h incubation time, a pH of 6.5 and a temperature of 40°C yielding a mannanase enzymatic activity of 5.13 U mL–1. When locust bean gum was added to the substrate composed of coconut and tofu waste, the best conditions were a dose of 0.6%, a 48 h incubation time, a pH = 6.5 and a temperature of 40°C yielding a 5.92 U mL–1 mannanase enzymatic activity. Conclusion: It is concluded that the activity of the mannanase enzyme with the substrate composed of coconut and tofu waste is not much different from that with the substrate containing locust bean gum, with the difference being only 13.34%.
 
 
 
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