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Articles by Wilart Pompimon
Total Records ( 6 ) for Wilart Pompimon
  Wilart Pompimon , Jinnantina Jomduang , Uma Prawat and Samlee Mankhetkorn
  Problem statement: Plant derived fungicides are now being subjects of many research groups. These secondary metabolites have enormous potential to inspire and influence modern agrochemical research. The study aimed to investigate the antifungal activity and their potential use as fungicides in the agriculture of crude extracts and purified compounds derived from plants used in traditional medicines.
Approach: Four medicinal plants including A. galanga, C. longa, B. pandurata and C. odorata were selected and percolated with hexane, ethyl acetate, acetone or methanol. The extracts were purified and elucidated their chemical structures. Disc mycelial growth inhibition was applied in order to determine their anti P. capsici activity and the field study was performed to determine their potential use in controlling fungal infection in chili plants compared with commercial fungicides such as captan and bio-control Trichoderma virens.
Results:
All crude extract inhibited mycelial growth of the fungus performed with similar efficacy. ED90 was equal to 300 ppm. Among plants studied B. pandurata was the most potent against P. capsici. The proposed active ingredients were pinostrobin and pinocembrin. In the field study, pinocembrin mediated the same anti P. capsici activity as captan. B. pandurata can protect chili from infection, thus increasing crop yield of chili comparable to Trichoderma virens.
Conclusion:
The results clearly showed that the extracts of the four plants studied could be considered as potential sources of novel fungicides. Particularly, B. pandurata has a very high potential as raw material for developing the antifungal molecule of non-petrochemical, naturally eco-friendly, easily obtainable and not toxic to human beings and environment, at least for use in chili growing.
  Sod Monkodkaew , Chatchanok Loetchutinat , Narong Nuntasaen and Wilart Pompimon
  Problem statement: Medicinal plants derived anticancer were now being subjects of many research groups especially, the secondary metabolite triterpenoids trees which had enormous potential to inspire and influence modern antiproliferative research. The study aimed to investigate the chemical constitution and their potential use as antiproliferative activity of purified compounds derived from F. virosa. Approach: The F. virosa was selected and percolated with hexane, ethyl acetate, acetone and methanol. The extracts were purified and elucidated chemical structures. Furthermore, the isolated compounds were tested for biological activity. The bioassays were performed on two cancer cell lines, adriamycin-sensitive erythroleukemia cells (K562) and adriamycin-resistant erythroleukemia cells (K562/Adr) which overexpressed P-glycoprotein (MDR1/ABCB1). Results: Friedelin (1), epifriedelanol (3), stigmasterol (4) and betulinic acid (5) were isolated from the leaves and twigs of F. virosa. The molecular structures of these compounds were determined using several spectroscopic methods. The compounds i.e., 1, a chemically modified compound 1 heptanolide (2), 3 and 4 showed a limited cytotoxic activity towards human cancer cell lines mainly due to a low aqueous solubility which prevented their use in cell viability assays. Interestingly, compound 5 exhibited a high cytotoxicity characterized by an effective concentration value (IC50) equal to 9.72.1 g.mL-1 (21.24.6 M) and 7.10.7 g.mL-1 (15.51.5 M) for K562 and K562/Adr, respectively. Moreover, the antiproliferative activity of compound 5 was independent of the multidrug resistance phenotype exhibited by the K562/Adr cell line suggesting that compound 5 was not the effluxes out of the K562/Adr cells by MDR1 (ABCB1). Conclusion: The results clearly showed that the betulinic acid of the four isolated compounds from F. virosa could be considered as high potential source of cytotoxic activity.
  Pradit Pradupsri , Chatchanok Loetchutinat , Narong Nuntasaen , Puttinan Meepowpan , Wirote Tuntiwechapikul and Wilart Pompimon
  Problem statement: Goniothalamus maewongensis is one of three new species which are found in Thailand recently. The genus goniothalamus is not only well known for the rich of styrylpyrones but also famous for the potential of biological activities, especially the cytotoxic activity against a number of human cancer cell lines. The phytochemical and biological investigations of this plant are interesting to bioassay-guided fractionation, particularly cell cycle arrest. Approach: The investigation was carried out to extract, isolate, purify and elucidate structure of the active compound from the leaves and twigs of Goniothalamus maewongensis. Both of the solvent extracts and isolated compound were evaluated with kinds of mammalian cancer cell lines, i.e., A549, GLC4, GLC4/Adr, K562 and K562/Adr for antiproliferation assay and cell cycle analysis. Results: Styrylpyrone from the leaves and twigs of Goniothalamus maewongensis was isolated from the active hexane extract. The spectroscopic techniques were provided for success in structure elucidation. In addition, a styrylpyrone compound was the most powerful to biological activities, which this molecular is significantly more toxic to small cell lung cancer than non small cell lung cancer cell (p<0.05). On the other hand, the goniodiol was not recognized by both multidrug resistance protein (ABCC1 and ABCB1). The study of cell cycle arrest explained antiproliferation effect by goniodiol at G2/M arrest in both lung cancer type (A549 and GLC4) and erythroleukemia cell (K562), while cell cycle arrest by goniodiol on both resistant cell lines are positioned on G0/G1 or S-phase. Conclusion: Goniodiol exhibits anti-proliferative on cancer cell line and un-recognized by multidrug resistant protein (ABCB1 and ABCC1).
  Sirinapa Nantapap , Chatchanok Loetchutinat , Puttinan Meepowpan , Narong Nuntasaen and Wilart Pompimon
  Problem statement: S. venosa (Menispermaceae) is used in traditional medicine. The constituents of S. venosa belonging to showed remarkable cytotoxic activity. According to previous research, S. venosa contains several alkaloids, such as protoberberine stephanine cyclanoline and N-methylstepholidine, kamaline, (+)-N-carboxamidostepharine, (-)-O-methylstepharinosine, (-)-stepharinosine, aporphine (-)-O-acethylsukhodiamine and oxostephanosine. The chemical and biological investigations of this plant are interesting to bioassay-guided fractionation, particularly Antiproliferative effects via the induction of cell cycle arrest in mammalian cancer cell lines. Approach: The research was carried out to extract, isolate, purify and elucidate structure of the active compound from the tuber S. venosa. Most of the solvent extracts and isolated compound were evaluated with kinds of mammalian cancer cell lines for investigation on antiproliferative effects. Results: Four alkaloids, tetrahydropalmatine (1), crebanine (2) O-methylbulbocapnine (3) and N-methyltetrahydropalmatine (4) were isolated from the tuber of S. venosa. Charaterization of the compounds were carried out by extensive NMR studies using COSY, HMQC, HMBC and DEPT in addition to other spectroscopic methods. These compounds (1, 2 and 3) were showed evidence of the anticancer activities for cell proliferation inhibition in K562, K562/Adr, GLC4 and GLC4/Adr cell lines due to G0/G1 obstruction by compound 2 and 3 and negligible S phase arrest by compound 1. Conclusion: The result showed slightly increase in S phase by the effect of compound 1, beside the G0/G1 phase was blocked by compound 2 and 3.
  Saksri Sanyacharernkul , Akanit Itghiarbha , Prachya Kongtawelert , Puttinan Meepowpan , Narong Nuntasaen and Wilart Pompimon
  Problem statement: Dregea genus (Asclepiadaceae) is well known for the rich of steroid pregnane contents and these plants are famous for the potential to be applied as alternative biological activities. Dregea volubilis is the only species of Dregea genus in Thailand. The chemical and biological investigations of this plant are interesting to bioassay-guided fractionation, particularly chondroprotective effect. Approach: The research was carried out to extract, isolate, purify and elucidate structure of the active compound from the roots Dregea volubilis. Both of the solvent extracts and isolated compound were evaluated with kinds of chondroprotection. i.e., S-GAG), HA, UA and production of matrix metalloproteinase-2 (MMP-2). Results: Polyoxypregnane glycoside (PGG) or 12-0-benzoyl-8, 11-ditigloyl-3β, 8β, 11α, 12β, 14β-pentahydroxy-pregn-14-ol, 20-one,-3-0-methyl-β-D-allopyranosyl (1→4)-β-D-thevetopyranoside was isolated from the active ethyl acetate extract of the roots Dregea volubilis. The spectroscopic techniques were provided for success in structure determination. In addition, a new compound was the most powerful to biological activities. Chondroprotective effect of PPG on the degradation of sulfated glycosaminoglycan (S-GAG), hyaluronan (HA), uronic acid (UA) and production of matrix metalloproteinase-2 (MMP-2) in interleukin-1β (IL-1β)-stimulated porcine articular cartilage were also assessed. PGG was interestingly effective in reducing IL-1β induced S-GAG, HA release from cartilage explant and MMP-2 activity. Furthermore, PPG can reverse effect of IL-1β-reduced the levels of uronic acid remaining in cartilage tissue. Conclusion: The PGG was possessed a potent chondroprotective activity using the IL-1β stimulated cartilage explant model. Therefore, it is possible to use this compound as a new pharmacological agent for the management of degenerative joint diseases.

  Patiwat Sakkrom , Wilart Pompimon , Puttinan Meepowpan , Narong Nuntasaen and Chatchanok Loetchutinat
  Problem statement: The Multidrug Resistance phenomenon (MDR) is the cause of unsuccessful in cancer chemotherapy. The tradition plant is the population used as the alternative medicine in cancer therapy. Due to, P. taxodiifolius is medicinal Thai plant which is used as diuretic drug which has never been explored as the anticancer activities. Approach: Air-dried powder of P. taxodiifolius leaves and twigs were serially extracted by hexane, ethyl acetate, acetone and methanol. These four extracts were tested the antiproliferative activity on four cancer cell lines. These results lead to successively purify two triternoids that are glochidone (1) and lupeol (2). Both pure compounds were tested the anticancer properties on same cancer cell lines and further investigate the cellular energetic state perturbation by measuring the mitochondrial membrane potential modification. Results: Four crude extracts were extracted and two triterpenoids (glochidone and lupeol) were purified and identified from hexane extract. Our antiproliferative activity of both compounds respectively showed in the IC50 value of K562, K562/Adr, GLC4 and GLC4/Adr equal to 2.2±0.6, 4.2±1.5. 3.1±1.0 and 3.2±0.9 μg mL-1 for glochidone and 2.3±0.6, 4.5±1.7, 2.3±0.5 and 2.6±0.5 μg mL-1 for lupeol. The R value, which represents the multidrug resistance phenotype, is about 2 for P-glycloprotein overexpression in K562/Adr and 1 for MRP1 overexpression in GLC4/Adr. Conclusion: All crude extractions and two triterpenoids show the clear evidence of anticancer activity of both sensitive and resistance of erythroleukemic and Small cell lung cancer cell lines. Both compounds are not recognized by ABCB1 and ABCC1 proteins. Our results also indicated that lupeol initiate cell death by mitochondria membrane potential modification specially the sensitive cell line.
 
 
 
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