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Articles by Wihandoyo
Total Records ( 11 ) for Wihandoyo
  Sri- Sudaryati , J.H.P. Sidadolog , Wihandoyo , W.T. Artama and D. Maharani
  Insulin-like growth factor binding protein 2 regulates a broad spectrum of biological activities involved in growth, development and differentiation. The current study was designed to investigate the associations of IGFBP2 gene polymorphisms with the variance components and genetic parameters of four weekly intervals of growth rate (0-4, 4-8, 8-12 weeks) of the Kampung chicken that were evaluated with classical models of quantitative genetics. Thirty two females and 16 males Kampung chicken were genotyped with IGFBP2 gene using PCR-RFLP method. Retrieved 3 genotype (CC, CT and TT) and 2 alleles (C and T). The influence of average effect of the C allele was greater than T allele on a 4-8 weeks intervals of growth rate, meanwhile for the males turn into 0-4 and 8-12 weeks interval growth rate. Ratio component additive and dominant deviation to each genotype is always equal for each interval growth rate. Variance component from heterozygote nearly zero when frequency of homozygote nearly equal and ratio variance dominance were opposite to allele frequency. Narrow-sense heritability based on genetic variance component of 0-4, 4-8 and 8-12 weeks interval growth rate for female were 0.01, 0.47 and 0.87 while for male chicken were 0.94, 0.07 and 0.12, respectively. It was concluded that IGFBP 2 gene association with growth rate on 4-8 and 8-12 weeks on females but in males only effect on growth rate on 0-4 weeks.
  B. Ariyadi , J.H.P. Sidadolog , S. Harimurti , S. Sudaryati and Wihandoyo
  This study was conducted to analyze the relationship between the condition of non-feather distributions and its genetically traits as a multiple allele of the feather condition in the Indonesian indigenous naked neck chickens. Eight males Indonesian naked neck chickens were paired with the five females that had the different condition of non-feathers distribution, namely non-feathers on the neck only (30%); non-feathers on the crop and breast (60%) and non-feathers on the neck, crop, breast and the back of body (90%). Assuming that the non-feathers distribution was caused by a multiple alleles of Na gene and developing of distribution were caused by Na+ as a multiple alleles of Na gene. This study revealed the effect of multiple allele Na+ on the distribution of non-feather areas in the chicken bodies. Analysis of inheritance was conducted by Mendelian heredity with chi-square (X2) analysis. The results, in the Indonesian naked neck chicken, showed that the distribution of non-feathers areas from the neck to the back of body might be caused by genetic of Na+Na+ and Na+Na. The non-feather distribution areas in the crop, breast and the thigh might be genetics effect of Na+na and NaNa (90%). The non-feather distribution areas in the neck or in the crops might be genetics effect of Nana. The normal feathers of chicken might be effect of recessive gene of nana. Moreover, the study showed that the Indonesian naked neck chicken had a lower productivity, higher embryonic mortality and lower hatchability.
  B. Ariyadi , J.H.P. Sidadolog , S. Harimurti , S. Sudaryati , Wihandoyo and H. Sasongko
  Background: The cecum of the chicken gut may be susceptible to pathogens because it is readily colonized by microbes. The lower segment of the gut is also the primary tissue that permits the invasion of microorganisms from the external environment and the cloaca. Mucins, which are composed of glycoproteins, play significant roles in forming the barrier against infection on the mucosal surface. Objective: The aim of this study was to determine the differences in the mucosal barrier of the lower segment of the gut between Indonesian naked neck chickens and normal feathered chickens. Methodology: The lower segments of the gut (rectum, colon and cecal tonsil) of Indonesian indigenous naked neck chickens and normal chickens were collected. The expression of the mucin 2 gene in the gut mucosa was analyzed by reverse-transcription-polymerase chain reaction (RT-PCR). The localization and molecular sizes of the mucosal glycoproteins were analyzed by Western blot. Wheat Germ Agglutinin (WGA) and jacalin lectins were used for Western blot analysis. Results: The mucin 2 gene was expressed in the mucosal gut of the rectum, colon and cecal tonsil in both naked neck chickens and normal chickens. Western blot analysis showed a single band for both WGA and jacalin from the mucosal gut of the rectum, colon and cecal tonsil in both naked neck chickens and normal chickens. Conclusion: These results suggest that the mucin 2 gene and glycoproteins containing WGA and jacalin positive sugars cover the surface of mucosal gut in both naked neck chickens and normal chickens, most likely to form a mucosa barrier.
  Deki Zulkarnain , Zuprizal , Wihandoyo and Supadmo
  Background and Objective: The dominant content of crude fibre is cellulose and if it is used as a feed ingredient, cellulase enzyme must be added. The aim of this research was to investigate the effect of using sago waste with cellulase enzyme as a local feed ingredient on the production performance of broiler chickens. Materials and Methods: Three hundred Day Old Chickens (DOC) broiler strain CP 707 were kept in enclosed litter cages. There were 30 cage plots. The size of each plot was (1×1) m2. The cage wall was made of metal. The wall height was 0.5 m. Each plot was equipped with a feeding and drinking container. There were10 DOC broilers in every plot with six replications. The treatment for this research was sago waste with cellulase enzyme at 0.75 g kg–1 sago waste. The sago waste is added at levels of 0.00, 5.00, 10.00, 15.00 and 20.00% of total feed. The variables measured in this research were feed consumption, body weight gain, feed conversion, the abdominal fat weight, the percentage of abdominal fat and fatty meat. The data were analyzed by using a one-way ANOVA with a Completely Randomized Design and if there was a difference between treatments (p<0.05), Duncan’s New Multiple Range Test (DMRT) was used. Results: The results of this research show that the addition of sago waste with cellulase enzyme in the feed did not significantly influence consumption and fat content of the meat of 35 days old broiler chickens but did significantly influence body weight gain, feed conversion, the weight of abdominal fat and percentage of abdominal fat of 35 days old broiler chickens (p<0.05). Conclusion: Sago waste with cellulase enzyme as a broiler feed ingredient can improve body weight gain as well as decrease feed conversion, the weight of abdominal fat and the percentage of abdominal fat. It is more effective if it is used at a level of 15% of total broiler feed and it does not have a negative effect on feed consumption and fat content of 35 days old broiler chicken meat.
  Charles V. Lisnahan , Wihandoyo , Zuprizal and Sri Harimurti
  Objective: The aim of this study was to determine the effect of the addition of methionine and lysine to feed based on cafeteria standards of native chickens on their growth performance (0-6 weeks). Materials and Methods: A total of 288 days-old native chickens (DOC) were used in this study. The DOC were divided into 4 treatments groups with 4 replications. The treatment diets were T0 and T1 (according to cafeteria and NRC standards) and T2 and T3, which were based on cafeteria standards with the addition of 0.14% methionine and 0.40% lysine for T2 and 0.27% methionine and 0.79% lysine for T3. The data collected were feed intake, body weight, feed conversion ratio and carcass as a percentage of body weight. Results: The results showed that the feed intake of T0, T1, T2 and T3 were 516.97, 556.91, 621.79 and 654.30 g/bird/6 weeks, respectively. The body weights for each group were 219.09, 232.67, 267.16 and 284.61 g/bird/6 weeks, respectively. The feed conversion data were 2.79, 2.80, 2.66 and 2.61, respectively and the carcass percentages were 53.20, 52.75, 54.63 and 56.85%, respectively. Conclusion: Feed formulated to cafeteria standards with the addition of 0.27% methionine and 0.79% lysine (group T3) resulted in the best growth performance.
  Cytske Sabuna , Wihandoyo , Sri Harimurti and R. Wisnu Nurcahyo
  Objective: The aim of this study was to quantify the bioactive components and antiparasitic activity of distillate citronella (Cymbopogon nardus or C. nardus) essential oil waste. Methodology: A densitometry method was used to quantitatively analyze the bioactive content of citronella waste. The effects of three concentrations of citronella waste extract and exposure time (30, 60, or 90 min) on adult Ascaridia galli (A. galli) worms in vitro from chicken intestines were evaluated. Results: Distillate waste from citronella (Cymbopogon nardus) (DWC) essential oil contains geraniol compounds. Treatment with DWC extract and powder significantly increased the mortality rate of A. galli compare to physiological solution (NaCl 0.9%). However, treatment with both 1.0% DCW and physiological solution (NaCl 0.9%) significantly increased the mortality rate of A. galli. Treatment effects increased significantly across all time points and there were interactions between material and concentration and; material and time points. Conclusion: The geraniol found in DWC may act as an antiparasitic against A. galli worms.
  Nur Widodo , Wihandoyo , Zuprizal and Nanung Danar Dono
  Objective: The aim of this study was to determine the effect of Binahong Leaf Meal (BLM) as a feed additive on the ileal bacteria and jejunal histomorphology of broiler chickens. Methodology: One hundred and ninety-two day old male broiler chickens were divided into six treatments groups of eight chickens each; each experiment was performed four times. The treatment groups were as follows: T0 (control negative), T1 (control positive; tetracycline 50 ppm), T2 (1% BLM), T3 (2% BLM), T4 (4% BLM) and T5 (8% BLM). Results: The addition of BLM significantly increased the amount of E. coli ileal bacteria, jejunum height and depth of jejunum crypts across groups T0-T5 (p<0.01). Addition of BLM significantly increased the amount of ileal lactic acid bacteria and width of jejunum villi across groups T0-T5 (p<0.05). Conclusion: The addition of 2% BLM increased the amount of E. coli ileal bacteria and ileal Lactic Acid Bacteria (LAB) and increased villi height and width and jejunum crypt depth in broiler chickens.
  Dyah Maharani , Wihandoyo , Galuh Adi Insani , Laurentia Adinda and Dwi Nur Happy Hariyono
  Objective: The study was carried out to characterize the qualitative morphological traits of Indonesian native chickens. Materials and Methods: Forty adult male chickens made up of 4 chicken groups based on different comb types (single, pea, rose and walnut) were visually appraised for the qualitative traits. Data on qualitative traits were analyzed using descriptive statistics and compared as percentages. Results: In single group, the majority of chickens have dark yellow beak (40%), red-brown crown (50%) and neck feather (50%), black for the feather of chest (50%), abdomen (50%), back (60%), primary (60%) and secondary wings (90%) and tail (40%). In Pea group, the majority of chickens possessed light yellow beak (50%), red-brown crown (40%) and neck feather (40%), white-brown chest feather (40%), black for the feather of abdomen (40%) and back (30%) and black-green tail (40%). In Rose group, most of chickens possessed light-yellow beak (40%), red crown (40%), yellow neck feather (50%), red for the feather of chest (40%) and abdomen (40%), black back feather (60%) and black-white tail (60%). In Walnut group, the majority of chickens have pale yellow beak (50%), red-brown crown (80%) and neck feather (60%), black for the feather of chest (40%), abdomen (40%) and back (30%) and black-white tail (40%). Dark eye, black primary and secondary wings, black thigh feather, light yellow membrane shank and yellow shank were predominant in all chicken groups. Conclusion: Indonesian native chickens possess a considerable phenotypic diversity that could be important for the genetic improvement through systematic breeding strategies.
  Deki Zulkarnain , Zuprizal , Wihandoyo and Supadmo
  Objective: The purpose of this study is to determine the amount of cellulase enzyme needed to optimize sago palm waste feed digestibility in vitro . This information can provide the basis for use of sago palm waste in broiler chicken feed. Methodology: Cellulase was added to the sago palm waste feed material at 5 different concentrations and five trials were conducted for each feed mixture. The parameters measured in this study were: in vitro dry matter digestibility (IVDMD), in vitro organic matter digestibility (IVOMD) and in vitro crude protein digestibility (IVCPD). The metabolic energy, crude fiber and cellulose contents in sago palm waste were also determined. The resulting data were analyzed statistically with a directional completely randomized design. Results: Sago palm waste with cellulase added to 0.75 g cellulase kg–1 waste had the best IVDMD (35.94 vs 19.32% for 0 g cellulase) and IVOMD (35.25 vs 19.32% for 0 g cellulase) values. Optimal values for the crude fiber and cellulose content were also seen for 0.75 g cellulase kg–1 waste with a maximum reduction to 12.79% crude fiber content (decreased by 23.69% compared to untreated waste) and 7.91% cellulose content (decreased by 29.56% relative to untreated waste). The crude protein digestibility and sago palm waste metabolic energy content at various cellulase concentrations were similar but 0.75 g cellulose kg–1 sago palm waste produced optimal crude protein digestibility and metabolism content. Conclusion: Addition of cellulase enzyme to sago palm waste at 0.75 g cellulose kg–1 sago palm waste improves IVDMD and IVOMD. This amount of cellulase also produced the best crude fiber and cellulose contents. Together these results suggest that cellulase can be used to improve the qualities of sago palm waste as a feed for broiler chickens.
  Charles V. Lisnahan , Wihandoyo , Zuprizal and Sri Harimurti
  Objective: This study was conducted to determine the effect of cafeteria standard feed supplemented with methionine and lysine on the growth performance of native chickens during the grower phase (6-14 weeks). Methodology: A total of 240 six week-old native chickens were randomly divided into 4 treatments and 4 replications, with 15 birds in each replicate. The dietary treatments were: T0 (feed based on cafeteria standard), T1 (feed based on protein standard of NRC), T2 (cafeteria feed+0.10% methionine+0.25% lysine), T3 (cafeteria feed+0.23% methionine+0.55% lysine). Feed consumption, body weight gain and feed conversion ratio were measured over the 8 week growing period. At the end of the feeding trial, 32 birds were slaughtered and carcass percentages were determined. Results: Feed consumption of T0, T1, T2 and T3 were 2,671, 2,628, 2,722 and 2,805 g/bird/8 weeks, respectively. The body weight gain for the respective treatments was 692, 677, 747 and 780 g/bird/8 weeks whereas the feed conversion ratio was 3.86, 3.70, 3.65 and 3.60. The percentage of carcass for the four treatments was 59.50, 59.66, 61.36 and 61.55%, respectively. Conclusion: Treatment T3 that had supplementation with 0.23% methionine and 0.55% lysine produced the best growth performance.
  C.S. Utama , Zuprizal , C. Hanim and Wihandoyo
  Objective: The aim of this study was to test the ability of Lactobacillus brevis and Lactobacillus plantarum derived from fermented cabbage waste juice to acts as probiotics. Materials and Methods: Tests of probiotic ability included tests of bile salt resistance and pH resistance and tests of sensitive inhibition of Escherichia coli and Salmonella pullorum growth. Results: Lactobacillus brevis and Lactobacillus plantarum derived from fermented cabbage waste juice were able to grow and develop at pH values from 2.5-5.5 and bile salt concentrations of 1-5%. Lactobacillus brevis was able to strongly inhibit Escherichia coli and Salmonella pullorum growth, while Lactobacillus plantarum showed very potent inhibition of Escherichia coli growth and potent inhibition of Salmonella pullorum growth. Conclusion: Lactobacillus brevis and Lactobacillus plantarum derived from fermented cabbage waste juice are suitable for use as poultry probiotics.
 
 
 
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