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Articles by Wesam I.A. Saber
Total Records ( 6 ) for Wesam I.A. Saber
  Mohammed S. El- Hersh , Wesam I.A. Saber and Husain A. El- Fadaly
  Bacillus subtilis ATCC 11774 was exploited to produce amino acids on a medium containing olive cake as non-traditional carbon and nitrogen source. Initially, the potential proteolysis of B. subtilis ATCC 11774 was sequentially optimized for protease production based on statistical strategy. In which, the two level Plackett-Burman design revealed peptone and olive cake as the significant factors affecting protease production. Optimization of both constituents in addition to pH using central composite design maximized protease production (30.3 U mL-1 min-1) by an overall 4.59-fold increase as compared to the non-optimized (6.6 U mL-1 min-1) medium, with marked evidence for the biosynthesis of total free amino acids, being 3403 μg mL-1, accompanied with the reduction in final culture pH down to 4.3. The differentiation of the filtrate of optimized protease production medium using amino acid analyzer revealed glutamic acid (1185.9 μg mL-1) as the highest synthesized amino acid. Other minor amounts of amino acids were also detected. Interestingly, this is the first report on the production of amino acids from olive cake-based medium.
  Wesam I.A. Saber , Noura E. El-Naggar , Mohammad S. El-Hersh and Ayman Y. El-Khateeb
  The ready available biomass of Rice Straw (RS) and Faba Bean Straw (FBS) cause serious environmental problems to several countries. A novel synergistic technique was applied using Aspergillus oryzae S2 in association with Azotobacter chroococcum to mediate the bioconversion of RS, FBS and Rock Phosphate (RP) into organic acids. Six-days of fermentation were optimal for improving C/N ratio and biodegradation of Fermented Biomass (FM) without previous pretreatment. Box-Behnken factorial design, with three independent variables: RP, (NH4)2SO4 and FBS has been adopted for multi-response surface optimization for biodegradation of FM, using the Desirability function (D) approach. Solving the obtained equation model, determines the optimum levels of the independent variables at 120 mg P from RP, 10.39 mg (NH4)2SO4 and 0.92 g FBS per 10 g RS. These levels were validated, the responses were 37.11% RDW and 17.13, 4.70 and 12.43 mg g-1 FM for total, soluble and insoluble sugars, respectively, with D value of 0.892, indicating the efficacy of biodegradation process. The HPLC analysis of the filtrate of the optimized FM contained (mg g-1 FM) 15.270 citric and 13.715 succinic acids as the major acids, beside minor amounts of ascorbic, oxalic, itaconic and maleic acids. The fungal strain was molecularly identified as A. oryzae S2 (KJ487973).
  Ibrahim A.A. Abou Ayana , Amal Elsady Ibrahim and Wesam I.A. Saber
  Microbial sources of bioagents are coinciding with the attempts to find cheap alternative substrates for high production. Rennet is the golden element in the dairy industry, particularly cheese making. Commonly, the use of statistical models to optimize culture medium components and conditions has increased in present-day biotechnology, due to its ready applicability and aptness. The present study adopted statistical approach for Milk Clotting Enzyme (MCE) production by Mucor mucedo KP736529 which was isolated, selected and molecularly identified, as the most potent active strain in MCE biosynthesis. Screening of various medium constituents using the statistical Plackett-Burman design showed glucose and KH2PO4 as the significant factors affecting MCE biosynthesis. Optimizing MCE production by the central composite design led to 6.12 fold increase in MCE production, compared with initial activity. Domiati cheese was manufactured using the M. mucedo KP736529 enzyme (E-cheese) or using commercial calf rennet (C-cheese) as control. During two months under pickling conditions, the yield and chemical properties of E-cheese were better than those of C-cheese. The fungal enzyme (MCE) showed proteolytic activity higher than calf rennet with the absence of taste defects such as bitter taste. Organoleptically, E-cheese scored the highest points.
  Khalid M. Ghoneem , Wesam I.A. Saber and Mohamed A. Elwakil
  Verticillium dahliae attacks a wide range of plants including fennel causing a wilt disease. The fungus grows slowly on seeds when tested at the seed health laboratories. This habit character allows saprophytes to impair the fungal growth and interfere the identification on both Moist Blotters (MB) and the Deep-Freezing Blotters (DFB). Since, these two techniques are not efficient enough to detect this fungus, the researchers planned to search for an alternative technique for detecting this fungus. Soaking three layers of blotters used as seed-beds in water solutions alkalined with KOH or NaOH at pH 10 presents the optimum seed-bed condition for manifesting the fungus on seed. This seed-bed condition also suppress the growth of saprophytes, so as the fungus was transparently shown on seeds. The in vitro study presents pH 9.5 as the optimum condition for the growth, sporulation and maximum glucose coefficient of the fungus. So far, it is recommended to use the alkalined seed-bed when searching for V. dahliae on fennel seed.
  Wesam I.A. Saber , Khalid M. Ghoneem , Mohammed M. El-Metwally and Mohamed A. Elwakil
  An emerging problem for the wider adoption of anise plantation in Egypt is the damage caused by the rust fungus. The detailed description and taxonomic studies (using light and scanning electron microscopy) show that such an obligate parasite fungus (Puccinia pimpinellae) is autoecious microcyclic (uredinial-telial stage only). Among tested Apiaceae plants, the host range test proved the specificity of the rust fungus to anise. To the researcher’s knowledge, this is the first investigated record of a rust fungus on Pimpinella anisum plants in Egypt. The effectiveness of some plant resistance elicitors and two active chitinase producers; Bacillus subtilis Bio4 and isolated Trichoderma harizianum CH4 (both of them recorded the highest clear zone/colony size ratio on chitin agar plates) in controlling anise rust disease and on growth and yield of anise were evaluated in two successive growing seasons. Spraying chitosan at 1000 ppm was the most potent in reducing Disease Severity (DS) and Incidence (DI) as well as improving plant height, chlorophyll content, inflorescence No. plant-1 (74.2 and 76), 1000-fruit weight (2.94 and 2.83 g) and anise yield (646.8 and 670.0 kg fed-1), during both seasons. B. subtilis Bio4 and T. harizianum CH4 showed moderate effect on the tested parameters.
  Noura El-Ahmady Ali El-Naggar , Mohammed S. El-Hersh , Husain A. El-Fadaly and Wesam I.A. Saber
  In view of an ever-increasing demand for oils and fats, there is an urgent need to develop non-conventional sources for its production, such as microbial sources. Single cell oil (SCO) production by strains of oleaginous yeasts i.e., Candida albicans NRRL Y-12983 and Lipomyces starkeyi NRRL Y-11557 grown on various agro-industrial by-products is a focus of current attention. The highest SCO productivity by both yeast strains was obtained at initial pH 6.0 and 25°C with agitation at 200 rpm after 72 h incubation. Sugar beet molasses and glucose syrup were the best inducers for SCO production by C. albicans NRRL Y-12983 and L. starkeyi NRRL Y-11557 (1.5 g L-1 and 1.4 g L-1 , respectively). Among nitrogen sources, corn gluten meal and protelan greatly induced SCO production by C. albicans NRRL Y-12983 and L. starkeyi NRRL Y-11557. (1.5 g L-1 and 1.6 g L-1, respectively). Palmitic (C16:0) and oleic (C18:1) acids were produced in highest values (20.544 and 54.261 mg g-1 sample, respectively) by C. albicans at 25°C; their values being 23.988 and 37.408 mg g-1 sample by L. starkeyi at 15°C. Linoleic acid (C18:2) was produced in highest amount of 41.302 mg g-1 by C. albicans at 15°C and 39.073 mg g-1 by L. starkeyi at 20°C. It can be concluded that oleaginous yeasts is a very efficient and inexpensive sources for SCO production. The ease, with which they can be cultivated, has facilitated the large-scale production of the microbial oil.
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