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Articles by Wenbin Bao
Total Records ( 2 ) for Wenbin Bao
  Shiping Zhu , Ying Liu , Wenhua Dong , Xianrui Zheng , Guoqiang Zhu , Shenglong Wu and Wenbin Bao
  To assess the feasibility of FUT1 gene M307 as a disease resistance genetic marker in Yorkshire pigs, this experiment adopted PCR-RFLP method to investigate the genetic variations at M307 of FUT1 gene in purebred Yorkshire (total 218 individuals), determined partial biochemical parameters and important cytokines using the automatic blood cell analyzer and ProCarta immunoassay kits and assayed a number of economic traits including eye muscle thickness, backfat thickness, growth rate and reproductive performance. This study also analyzed the relationship between polymorphism of FUT1 gene M307 and partial immune indexes, vital economic traits. The results showed that there were three genotypes (AA, AG and GG) in Yorkshire test groups, in which the allele G was dominant one. FUT1 gene M307 deviated from Hardy-Weinberg equilibrium (p<0.01) in the population. Correlation analysis showed that the level of thrombocytocrit (PCT) in AG, GG genotype individuals were significantly higher than that in AA genotype individuals (p<0.05), while there were no significant difference between AG and GG genotypes (p>0.05). The individuals with GG genotype were significantly higher than those with AG genotype (p<0.01) in IFN-γ, TGF-β and TNF-α. In terms of the age at 100 kg, individuals with AA type were significantly higher than those with AG type (p<0.05), the average age at 100 kg of AA type individuals was 4.23 days less than GG type individuals. From the 3rd-5th parity, the Total Number Born (TNB) and Number Born Alive (NBA) demonstrated a trend of AA>AG>GG, individuals with AA type were significantly higher than those individuals with GG type (p<0.05) in TNB and NBA at 3rd and 5th parities. These results indicated that AA genotype, as an ETEC F18 resistance genetype, would also improve growth rate and reproduction performance of Yorkshire pigs, but reduce their general disease resistance ability.
  B.C. LI , H. Chen , X.J. Xiao , Wei Han , Qi Xu , Wu Xinsheng , Wenbin Bao and G.H. Chen
  The objective of this study was to evaluate the effect of three cryorotectants (at three concentration each) and two cryopreservation protocols on the preservation of chicken Primordial Germ Cells (PGCs) from gonads at stage 19 and stage 28. The PGCs were cryopreserved using Dimethylsulphoxide (DMSO; 10%, 20%, 30%), glycol (GLY; 10%, 20%, 30%), polyethylene glycol (PEG; 10%, 20%, 30%). In a first series of experiments, we compared viability after three cryorotectants based protocol I and found the viability of PGCs showed very significant percentage (86.53%) (p<0.01) in a freezing media IV. We then compared an cryopreserve protocol I with 5% dimethyl sulfoxide (DMSO)+5% Glycol versus a cryoprotectant protocol II and observed a better viability with the former protocol (85.9% versus 67.4%, p<0.05). Finally, we compared viability of PGCs at three concentration each cryorotectant and found no significant difference p>0.05 between the concentration of 10% and 15% except freezing media III. When the concentration was 20%, the viability of PGCs was the lowest and showed significant difference p<0.05 or very significant difference (p<0.01) compared to other concentration. In conclusion, 5% DMSO+5%GLY with cryopreserve protocol I was the most effective cryopreservation for chicken primordial germ cells.
 
 
 
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