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Articles by Weiwei Zhang
Total Records ( 5 ) for Weiwei Zhang
  Lanlan Wang , Jiaping Yan , Xiangxiang Meng , Jiabao Ye , Weiwei Zhang and Feng Xu
  Background and Objective: Ginkgo biloba (G. biloba) is a precious medicinal and edible plant. It has a long juvenile phase. CONSTANS (CO) and CONSTANS-like (COL) genes are key genes in the photoperiodic flowering pathway. Information on COL genes in G. biloba is relatively lacking. The aim of this study was to characterize a CONSTANS-like 16 (GbCOL16) gene from G. biloba. Methodology: In this study, GbCOL16 gene was cloned from G. biloba. The expression level of GbCOL16 gene in different tissues of G. biloba was studied by semi-quantitative RT-PCR and quantitative RT-PCR methods. Data were analyzed with one-way ANOVA using SPSS 11.0 for windows. The means were compared with Duncan’s multiple range tests. Results: Sequence analysis results showed that the full-length cDNA of GbCOL16 was 1,337 bp, it contained a 1,311 bp ORF and encoded a deduced protein of 436 amino acids. The GbCOL16 has one conserved CCT domain, without B-Box domain. GbCOL16 has a close genetic relationship with SlCOL16 and NtCOL16 and can be clustered into group IV of CO gene family. The expression analysis results showed that the highest GbCOL16 expression was in the leaves. The GbCOL16 expression was higher in male strobili than in the stems, female strobili and young fruits. The lowest relative expression of GbCOL16 was in the roots. However, GbCOL16 barley was expressed in Ginkgo buds. Conclusion: GbCOL16 was expressed specifically in the leaves of ginkgo. The flowering regulation mechanism of GbCOL16 is similar to that of CO genes. This finding lays the foundation for clarifying the flowering gene network and molecular regulation mechanism of G. biloba.
  Li Zhu , Liangqiong Ma , Feng Xu and Weiwei Zhang
  Background and Objective: Ilex cornuta (I. cornuta) is a medicinal plant that contains triterpenoid compounds as its pharmacologically active ingredients. Squalene epoxidase (SE) is a key enzyme in the triterpenoid biosynthesis pathway. The current study aimed to characterize a SE gene from I. cornuta. Methodology: IcSE2 was isolated from I. cornuta. It was predicted the secondary and tertiary structures of the IcSE2 protein, performed multiple sequence alignments and generated a phylogenetic tree. The expression level of IcSE2 was examined via Quantitative Real Time-PCR (qRT-PCR). Results: The cDNA sequence of IcSE2 was 2058 bp, with an open reading frame of approximately 1605 bp that codes for 534 amino acids. The predicted theoretical isoelectric point and molecular weight are 8.30 and 58.6 kDa, respectively. RxR, Flavin Adenine Dinucleotide (FAD) and Nicotinamide Adenine Dinucleotide Phosphate (NADPH) domains could be found in the deduced IcSE2 protein. Phylogenetic analysis showed that IcSE2 is closely related with the SEs from Araliaceae plants. IcSE2 expression level was the highest in roots, followed by leaves and male flowers and lowest in stems. Conclusion: The IcSE2 gene from I. cornuta was cloned and characterized for the first time. IcSE2 was strongly expressed in roots and leaves. These results may lay the foundation for studying the molecular regulatory mechanism of triterpenoid saponins in I. cornuta.
  Xiaomeng Liu , Xiangxiang Meng , Jiabao Ye , Weiwei Zhang , Jie Chang and Feng Xu
  Background and Objective: Flavonoids are one kind of the main active ingredients in medicinal plant of Chamaemelum nobile (C. nobile). Chalcone synthase (CHS) is the first key enzyme and plays an important role in flavonoid biosynthesis pathway. The aim of the study was to clone the cDNA sequence of CnCHS from C. nobile for the first time using RT-PCR and carried out bioinformatics analysis. Materials and Methods: The seeds of C. nobile was obtained from the laboratory group's early preservation sown in a nutrition bowl (20×20 cm) after immersion and germination on November 10, 2016 and was grown at 25/18°C in a controlled growth chamber (16 h light/8 h dark). A pair of specific primers was designed according to C. nobile transcriptome data and the CnCHS gene was cloned from C. nobile by Reverse Transcription-Polymerase Chain Reaction (RT-PCR). The CnCHS gene as well as the protein sequence were analyzed using the online website of National Center for Biotechnology Information(NCBI), ProtParam and bio analysis software of Vector NTI 11.5, Clustal X2.0 and MEGA6. The expression of CnCHS gene in different tissues of C. nobile was studied by quantitative RT-PCR (qRT-PCR). Data were analyzed with one-way ANOVA using SPSS11.0 for Windows. Results: The cDNA of 1,482 bp CnCHS gene was cloned and named CnCHS (GenBank accession No. MF069253). The CnCHS contained a 1,197 bp open reading frame (ORF) encoding 399 amino acids. The predicted molecular weight and isoelectric point of the CnCHS protein were 43.64 kDa and 6.11, respectively. Multiple alignment analysis revealed that CnCHS protein sequence was highly homologous to CnCHS proteins of other plants. Phylogenetic analysis revealed that the CnCHS was most closely related to the CnCHS of Asteraceae, indicating that they share a common evolutionary ancestor. The CnCHS gene was expressed in all tested tissues of C. nobile and the expression level was the highest in flowers. Conclusion: The CnCHS gene was isolated and characterized, laying a foundation for further study of flavonoid biosynthesis pathway in C. nobile.
  Jingjing Liao , Xiaomeng Liu , Xian Zhou , Zexiong Chen , Junpin Tan , Jiabao Ye , Weiwei Zhang and Feng Xu
  Background and Objective: Ginkgo biloba is a precious medicinal plant and has a long juvenile phase and spends 15-20 years in the vegetative phase before turning to reproductive phases, which makes breeding and cultivation of Ginkgo especially challenging. The FRI gene can regulate the FLC gene which inhibits flowering and further causes the late flowering of G. biloba. Therefore, the cloning and analysis of FRI gene can regulate the flowering time of G. biloba. Materials and Methods: The GbFRI gene and the protein sequence were analyzed using the online website of National Center for Biotechnology Information (NCBI), ProtParam and bioinformatic software of Clustal X2.0, Vector NTI 11.5 and MEGA6. The expression of GbFRI gene in different tissues of G. biloba was studied by quantitative RT-PCR (qRT-PCR). Data were analyzed with one-way ANOVA using SPSS11.0 for Windows. Results: The full length cDNA of GbFRI gene was 1702 bp (GenBank accession no. KY662058) and the open reading frame (ORF) covered 1602 bp, which encoded a 534 amino-acid protein. The predicted protein showed that a FRI superfamily and contain coiled-coil domains in two positions (between amino acids 55-100 and 405-450, respectively). The expression analysis results displayed that the highest GbFRI expression was in the male flowers. The GbFRI expression was higher in female flowers, stems than in the roots and fruits. The lowest relative expression of GbFRI was in the leaves. Conclusion: The GbFRI gene was isolated and characterized, laying a foundation for further study of vernalization pathway in G. biloba.
  Jianguang Liu , Danfeng Sun , Feng He , Weiwei Zhang and Xiaoke Guan
  This study attempted to develop a low-cost, high-precision method to acquire land use/cover data by combining multi-temporal and multi-spectral Moderate Resolution Imaging Spectroradiometer (MODIS). The results indicate that Classification and Regression Tree (CART) algorithm clearly outperforms the Maximum Likelihood (ML) in land use/cover classification using MODIS and the first principal component (PC1) with multi-spectral MODIS image that reflected more soil information can efficiently improve the accuracy of classification based on MODIS NDVI time series.
 
 
 
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