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| Articles
by
Weihua Li |
Total Records (
3 ) for
Weihua Li |
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Zhenhua Gong
,
Lijuan Zhang
,
Ping Lu
,
Yonghao Hu
,
Liping Wang
and
Weihua Li
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Marek’s Disease (MD) is one of the main infectious diseases
that threaten the healthy development of the Chinese poultry industry. The evolution
of epidemic strains of the MD Virus (MDV) in China is different from the evolution
of strains overseas. The meq gene sequence of pathogenic strains is different
from that of the attenuated vaccine strain (MDV-1). The meq and L-meq
gene sequences of the MDV in the lymphoid tissue of chickens in latency period
are homologous. The meq gene was only found in the lymphoid tissue of
chickens during the onset period. |
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Weihua Li
,
Yang Li
,
Bohua Zhang
,
Xuan Dong
,
Zhizhong Cui
and
Peng Zhao
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To investigate the Reticuloendotheliosis virus infection status
in large-scale breeder chicken farms of China, 563 serum samples from 4 great-grandparents
breeder farms, 6557 serum samples from 8 grandparents breeder farms, 312 serum
samples from parents breeder farms, totally 7432 serum samples were collected
and examined using the Reticuloendotheliosis Virus Antibody Test kit. The results
showed that REV-antibody positive rates of great-grandparents breeder flocks,
grandparent breeder flocks and parents breeder flocks were 0-31, 0-47 and 4.23-42.50%,
respectively. REV-antibody positive rate has close relationship with incidence
of REV infection history and tumor complaints. This study suggests that REV
infection is very common in China large-scale breeder chicken farms and the
prevention of REV should be given more attention this is the largest scale serological
survey for breeder chickens in China so far. |
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Xin Fu
,
Weihua Li
,
Jian Zhang
and
YingHui Xu
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To study the role of P21/53 in directly regulating and controlling
TIMP-2 and the important mechanism for promoting invasion of GSCs by P21/53.
Transwell in vitro invasion experiment was used to test invasion ability
of GSCs and GCs as well as effect of P21/53 on GSCs invasion. Techniques of
qRT-PCR and Western blot were used to test and transfect expression variation
of TIMP-2, MMP2 and MMP9 in GSCs of P21/53 inhibitor. The luciferase reporter
gene experiment was used to test the role of P21/53 in directly regulating and
controlling TIMP-2. The number of invasive GSCs significantly decreased following
transfection of P21/53 inhibitor (p<0.05). The expression levels of TIMP-2
mRNA in the U87 cell line and GSCs from the two primary glioma specimens rose
compared with the control group, respectively while the expression levels of
MMP2 mRNA and MMP9 mRNA fell compared with the control group (p<0.05) following
transfection of P21 inhibitor. Expression of protein level of TIMP-2, MMP2 and
MMP9 were consistent with mRNA level and had significant difference compared
with those of the control group following transfection of P21 inhibitor and
P53 inhibitor. The luciferase activity of the reporter vector containing wiled-type
TIMP-2-3'UTR increased significantly following transfection of P21/53 while
the luciferase activity of the reporter decreased significantly following transfection
of P21/53 simulator (p<0.05). High expression of P21/53 promotes invasiveness
of GSCs and its mechanism is achieved by down-regulating expressions of TIMP-2
and up-regulating expressions of MMP2 and MMP9 as a minimum. |
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