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Articles by Wei Han
Total Records ( 3 ) for Wei Han
  B.C. LI , H. Chen , X.J. Xiao , Wei Han , Qi Xu , Wu Xinsheng , Wenbin Bao and G.H. Chen
  The objective of this study was to evaluate the effect of three cryorotectants (at three concentration each) and two cryopreservation protocols on the preservation of chicken Primordial Germ Cells (PGCs) from gonads at stage 19 and stage 28. The PGCs were cryopreserved using Dimethylsulphoxide (DMSO; 10%, 20%, 30%), glycol (GLY; 10%, 20%, 30%), polyethylene glycol (PEG; 10%, 20%, 30%). In a first series of experiments, we compared viability after three cryorotectants based protocol I and found the viability of PGCs showed very significant percentage (86.53%) (p<0.01) in a freezing media IV. We then compared an cryopreserve protocol I with 5% dimethyl sulfoxide (DMSO)+5% Glycol versus a cryoprotectant protocol II and observed a better viability with the former protocol (85.9% versus 67.4%, p<0.05). Finally, we compared viability of PGCs at three concentration each cryorotectant and found no significant difference p>0.05 between the concentration of 10% and 15% except freezing media III. When the concentration was 20%, the viability of PGCs was the lowest and showed significant difference p<0.05 or very significant difference (p<0.01) compared to other concentration. In conclusion, 5% DMSO+5%GLY with cryopreserve protocol I was the most effective cryopreservation for chicken primordial germ cells.
  Wenqi Zhu , Kuanwei Chen , Huifang Li , Weitao Song , Wenjuan Xu , Jingting Shu and Wei Han
  The systematic study of genetic diversity and origin of Chinese indigenous geese will provide an important scientific basis for the conservation, utilization of the resource and human history. The 521 bp control region (D-loop) of mitochondrial DNA from 13 grey goose breeds collected from conservation farms and zones was sequenced. The results showed the average Haplotype Diversity (Hd) and nucleotide diversity (Pi) of domestic geese were 0.19245 and 0.00036, respectively.
  Huifang Li , Wenqi Zhu , Kuanwei Chen , Weitao Song , Jingting Shu and Wei Han
  Alleles of physiological candidate genes for reproductive traits, Growth Hormone Receptor (GHR) and Insulin-like Growth Factor-I (IGF-I) were assessed to determine the association with Haugh Unit (HU), egg weight EGW, Egg Shell Weight (ESW), Egg Shell Strength (ESS) and Egg Shell Thickness (EST) at 55-week age of Wenchang chicken (Chinese indigenous breed). PCR-RFLP technique was applied to analyze the polymorphisms of GHR intron 5 and IGF-1 of Wenchang chickens. The effects of GHR intron 5 and IGF-1 on the egg quality were analyzed. The results showed that the allele frequencies of C, D were 0.19, 0.81 for GHR-Intron 5 and the allele frequencies of A, B were 0.53, 0.47 for IGF-1, respectively. Three significant were observed for GHR and EST; for IGF-1 and ESW and for IGF-1 and ESS.
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