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Articles by Wayan Tunas Artama
Total Records ( 5 ) for Wayan Tunas Artama
  Suhendra Pakpahan , Wayan Tunas Artama , Rini Widayanti and I. Gede Suparta
  Background: Hutan Sumatera goat (Sumatran serow) is one of wildlife that is quite similar to the domesticatic goats and have never been investigated at the molecular level. Indonesian society believes that Hutan Sumatera goat belongs to the group of the genus Capra, therefore, this animal is called a wild goat or forest goat. Hutan Sumatera goat has been categorized as an endangered species in the IUCN as red list. Materials and Methods: The genomic DNA of Sumatran serow and Indonesian goat were isolated by gSYNC DNA Mini Kit (Geneaid) and cytochrome b gene was amplified using PCR method with a pair of primer. Cytochrome b gene region was amplified completely from all samples with a length 1140 bp. Results: Alignment results show that there are many nucleotide differences between Hutan Sumatera goat and Indonesian domestic goats. Translation nucleotide results indicate that there are 11 different amino acids between Hutan Sumatera goat and domestic goats except with JAW (12 amino acids) and GS (10 amino acids). The percentage of A+T and G+C on Hutan Sumatera goat is 56.4 and 43.6%, whereas the Indonesian domestic goat is 58.6 and 41.4%. Using the restriction enzyme BamHI results in differences fragments between Hutan Sumatera goats and domestic goats. In the Cyt b gene Hutan Sumatera goat was cut on site 561 bp to produce two DNA bands, while in the domestic goat there is no cutting of DNA fragments at the site. Conclusion: The genetic distance between Hutan Sumatera goats ranged from 0.001-0.004, while the Hutan Sumatera goat to domestic goat ranged from 0.001-0.118. Based on a phylogenetic tree analysis, Hutan Sumatera goat form a group with genus Capricornis. This suggests that goat Hutan Sumatera goat is a part of the genus Capricornis.
  Suhendra Pakpahan , Rini Widayanti , Wayan Tunas Artama and I. Gede Suparta Budisatria
  Background: The coat color is important for the production of wool, for heat tolerance in hot climates, cultural value and for the identification of the breed. Goat has very important roles in the agricultural economies of Indonesia. The goat is not only to produce meat, milk, wool and compost, but also for cultural and religious events. Batak tribe in North Sumatra has a unique tradition of customary celebration by using goat. Goat that has completely white coat should be used, so that the white coat has economic value much higher than other colors. Materials and Methods: This study used blood from each individual. Four subpopulations of domestic goat breeds in Indonesia, namely Samosir goat, Kacang goat, Gembrong goat and Peranakan Etawah goat. The agouti signaling protein (ASIP) gene fragments amplified and sequenced. The length of the ASIP gene amplification products was 610 bp for every individual and analyzed to detect variations on every Indonesian local goat. Results: From all samples of Indonesian goats, there is only one difference on Samosir goat 1 c.158 G-T. This polymorphism only foundin Samosir goat 1 while the other Indonesian local goats and other organisms were the similar. This variation would change to the order of amino acids in goat Samosir 1. Conclusion: There is no variation in the ASIP fragment of Indonesian goats that can be used to investigate the relationship between exon 4 of ASIP and coat color expression of Indonesia local goats. The exon 4 of the ASIP cannot be made target to investigate different coat color in some Indonesian goat breeds.
  Suhendra Pakpahan , Wayan Tunas Artama , Rini Widayanti and I. Gede Suparta
  Indonesia has several breeds of goats as the source of genetic. This study aimed to characterize genetic of Indonesian goats. The phylogenetic relationship among and genetic variability within eight native Indonesian goats were analyzed using mtDNA D-loop region sequences. The results show that the length of complete sequences mtDNA D-loop in Indonesian goats are1212 bp. There are 68 polymorphic sites and 21 different haplotypes. Haplotype diversity and nucleotide diversity based on mtDNA D-loop in eight breeds of Indonesian goats are 0.5000-1.000 and 0.248-1.1595%, respectively. The value of genetic distance between groups of native Indonesian goats ranged from 0-0.011. Indonesia goat genetic distance value against wild goat Capra aegagrus (AB004082) is in the range 0.0038-0.045 while against Capra falconeri (AB044306) is 0.087-0.095. The results indicate that the genetic diversity of Indonesian goats is not abundant. Indonesian goats originated from one maternal. Maternal origin of Indonesian goats based on mtDNA D-loop is lineage B.
  Suhendra Pakpahan , Wayan Tunas Artama , Rini Widayanti and I. Gede Suparta
  Phylogenetic relationships and genetic variation among 9 Indonesian goat breeds were analyzed using cytochrome b gene sequences. The genomic DNA was isolated by gSYNC DNA Mini Kit (Geneaid) and amplified cytochrome b gene using PCR method with a pair of primer. Complete sequences of Cyt b genes of Indonesian goats is 1140 bp contained 19 variable sites and 8 haplotypes between Indonesian goats and C. hircus (D84201), when compared to C. aegagrus and C. falconeri, there are 59 variable sites. Phylogenetic analysis of haplotype in the combination with the goats from GenBank showed that Indonesian goats are grouped with C. aegagrus. Indonesian goat breeds have 4 amino acid units, which are different with C. hircus, at site 17, 191, 215 and 341th amino acids. The genetic distance score between Indonesian goat populations ranged from 0 to 0.007, while if compared to C. aegagrus ranged from 0.002 to 0.006 and to the C. falconeri ranged from 0.039 to 0.045. The relationship between subpopulation of Indonesian goats are very close. Indonesian goats have a closer genetic relationship with wild goat C. aegagrus than to C. falconeri.
  Enny Yusuf Wachidah Yuniwarti , Widya Asmara , Wayan Tunas Artama and Charles Rangga Tabbu
  The purpose of this study was to increase the viability of avian influenza infected chicken through a Virgin Coconut Oil (VCO) administration in feed. A number of live chicken and chicken duration of survival after the challenge test with virus influenza A subtype H5N1 and supporting of immunological data including antibody titers, lymphocyte count, CD4 count and number of CD8 have been analysed for the effect of VCO. This study was conducted in a biosafety level 3 (BSL 3) laboratory using twenty-four broiler chickens that were divided into two groups: vaccinated AI and unvaccinated AI. All groups were fed with the supplementation of VCO (10 mL kg-1 of feed). The chicken without administration of VCO was used as a control. The results showed that supplementation of the VCO could increase chicken viability, antibody titers, lymphocyte count, CD4 count and number of CD8 after challenge test against AI virus.
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