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Articles by Wasu Pathom-Aree
Total Records ( 4 ) for Wasu Pathom-Aree
  Duthathai Fujaroen and Wasu Pathom-Aree
  Thermotolerant acetic acid bacteria were isolated from 15 kinds of flower namely Allamanda cathartica L., Antigonon leptopus Hook.t Arn., Bidens bipinnaata L., Brunfelsia hopeana Benth., Cassia surattensis Burm.f., Ervatamia divaricata, Euphorbia milii Desmoul., Ixora lobbii Loud., Jasminum multiflorum, Lantana trifolia L., Lonicera japonica Thun., Nerin indicum Mill., Petrea volubilis L., Plumeria acutifolia and Zinnia angustifolia Kunth. using potato medium supplemented with 4% ethanol (v/v) as an enrichment medium. Three different dyes namely 0.0016% bromocresol green, 0.0016% bromocresol purple and 0.0016% bromophenol blue were also incorporated in the enrichment broth for comparison. The numbers of successful isolation obtained from each dye were nearly the same and there were no significant differences between the dyes tested (α>0.05). However, the use of 0.0016% bromocresol purple as indicator for the isolation of thermotolerant acetic acid bacteria from flowers was recommended due to its most easily observed colour change. Morphological and biochemical determinations indicated that most of thermotolerant isolates obtained were members of the genus Gluconobacter.
  Amornrut Moryadee and Wasu Pathom-Aree
  Sixty thermotolerant acetic acid bacteria were isolated from 13 kinds of fruit using sterile distilled water supplemented with 4% ethanol (v/v) as an enrichment medium. Successful isolations were obtained from apple, Jamaican cherry, longan, mango, pineapple and rambutan. Morphological and biochemical examinations revealed that 43 isolates were members of the genus Acetobacter whereas the remaining 13 isolates were members of the genus Gluconobacter. Preliminary screening showed that isolates No. 13, 34, 36 and 37 gave the widest zone of acidity on overoxidation medium. These isolates were identified as A. aceti and selected for acetic acid production at 30 and 37°C by shaking culture for 14 days in ethanol-yeast extract medium. It was found that A. aceti isolate No. 37 from rambutan gave the highest acetic acid yield of 13.53 and 8.97 g L-1 at 30 and 37°C, respectively after 7 days of fermentation.
  Supawadee Sudsakda , Warinda Srichareon and Wasu Pathom-Aree
  The effectiveness of three enrichment broths, namely potato medium, seed culture medium and sterile distilled water for the isolation of thermotolerant acetic acid bacteria from flowers and fruits was investigated. The numbers of successful isolation obtained from each medium were nearly the same. Seed culture medium was found to give the highest numbers of successful isolation from flower samples whereas potato medium and sterile distilled water gave higher numbers for the isolation from fruits. However, there were no statistically significant differences between the media tested (α>0.05).
  Intira Thampayak , Naowarat Cheeptham , Wasu Pathom-Aree , Pimporn Leelapornpisid and Saisamorn Lumyong
  Two hundred and twenty-nine soil actinomycete strains were initially screened for extracellular biosurfactant activity by a drop-collapse method in Kim`s medium containing sesame oil as a sole source of carbon. Three isolates, namely S71, S72 and S177, were capable of biosurfactant production. Phenotypic and genotypic analysis strongly suggested that they were members of the genus Streptomyces. The isolates S71 and S177 were closely related to S. griseoflavus sharing 99% 16S rRNA gene similarities, whereas S72 was closely related to S. fradiae sharing only 98% 16S rRNA gene similarities suggesting that this may represent a novel species. The cell-free culture broth of the three isolates had emulsification activity and decreased surface tension. According to emulsification activity (E24) and surface tension values observed in the three isolates, Streptomyces sp. S72 was selected for biosurfactant production in larger scale. The cell-free culture broth of the isolate S72 was further extracted with chloroform:methanol (2:1) and two fractions were found positive in producing biosurfactants. To determine structure and molecular weight of the two positive fractions, the Nuclear Magnetic Resonance (NMR) spectroscopy and Mass Spectrometry (MS) will be carried out.
 
 
 
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