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Articles by Wasmen Manalu
Total Records ( 10 ) for Wasmen Manalu
  Tyas Rini Saraswati , Wasmen Manalu , Damiana Rita Ekastuti and Nastiti Kusumorini
  An experiment was conducted to study the effects of period of turmeric powder supplementation and ration quality on egg production in quails. One hundred and fifty female quails were assigned into a completely randomized design with a 2 x 5 factorial arrangement. The first factor was ration quality consisted of two levels i.e., ration with high carbohydrate (standard protein) and ration with high protein (standard carbohydrate) contents. The second factor was a period of turmeric supplementation (at a dosage of 54 mg/quail/day) consisted of 5 levels i.e. (1) Without turmeric supplementation (control) (2) 30 days at the age of 14 to 44 days (3) 30 days at the age of 45-75 days (4) 30 days at the age of 7-8 months and (5) 8.5 months at the age of 14 days to 9 months. Each experimental unit consisted of 15 quails. Parameters measured were feed and water consumptions, total number of egg production, follicle hierarchy, liver functions, oviduct weight and length and blood metabolites. The results showed that quails supplemented with turmeric for 30 days prior to sexual maturity and for 8.5 months started before sexual maturity gave the highest egg production (20 and 17%, respectively), liver function, vitellogenin secretion, follicle development and blood metabolites. Ration with high carbohydrate with standard protein content gave the best egg production, liver function and blood metabolites. It was concluded that improvement of liver functions could increase total egg production by increasing yolk precursors synthesis and their depositions in the developing follicles.
  Widya Hermana , Toto Toharmat , Sumiati and Wasmen Manalu
  Katuk (Sauropus androgynus) and mulberry (Morus alba) leaves meal contain cholesterol, campesterol, stigmasterol, sitosterol and 2-4alpha-methylsterol. These active substances were expected to increase nutrients metabolism in laying poultry including quail. Poultry in layer period have high nutrients requirement and mobilization. Usage of Katuk and mulberry leaves meal in the diet was expected to increase egg quality. Four dietary treatments and 5 replications of 15 quails of 6 weeks of age each, were allocated in a completely randomized design. Parameters observed were feed consumption, egg weight, quail day production and egg quality. The results showed that feed consumption and egg weight were not affected by the treatments, while egg production was significantly decreased by feeding the 10% mulberry leaf meal as well as the mixture of 5% Katuk and mulberry leaf meal (P < 0.05). Feeding 10% Katuk leaf meal did not affect the performances of the quail but the egg indicated the highest vitamin A and yolk colour score. It was concluded that 10% Katuk leaf meal could be fed to the laying quail to increase the egg quality without decreasing the production.
  Tyas Rini Saraswati , Wasmen Manalu , Damiana Rita Ekastuti and Nastiti Kusumorini
  This research was designed to study the profile of the hormones estriol, progesterone, in the blood during a cycle of ovulation in controlled laying hens and laying hens that treated by giving the turmeric powder. Determination of hormone profile was done on laying hens. Six laying hens aged 20 month were divided into two experimental unit. Each experimental unit consist of three laying hens i.e., P0: control (not supplemented with turmeric powder), P1: turmeric powder supplementation at a dose of 405 mg/laying hens/day for a month. Blood was drawn via jugular vein by 4 mL. Blood sampling performed every 2 h starting after hen lay eggs during a cycle of ovulation, then retrieved and analyzed the serum levels of estrogen and progesterone. The data graphed to determine the profile of the hormones estrogen, progesterone and compared between the controlled and the treated with turmeric powder. The results showed that administration of turmeric powder can shorten the cycle of ovulation about 5 h 35 min. Turmeric powder did not affect the pattern of fluctuations in the hormone progesterone. There are 4 progesterone peaks during the first cycle of ovulation both in controlled laying hens and laying hens that treated with turmeric powder. There was a change to the hormone estriol fluctuations. In controlled laying hens takes four peaks hormone estriol in an ovulatory cycle, whereas in laying hen treated with turmeric powder was 1 hormone estriol peak occurring approximately 15 h before ovulation.
  Andriyanto , Intan Maria Paramita , Dedi Nur Aripin , Pramesti Nugraheni , Zulfitra Utami Putri , Laras Agustanti , Rizka Septarina Budianti , Ridi Arif , Diah Nugrahani Pristihadi , Aulia Andi Mustika , Abadi Sutisna and Wasmen Manalu
  This research was conducted to study the effects of administration of Jamu jahkenkun, a combination of ginger, curcuma and turmeric preparation, on productivity and hematological profile of broiler chickens. One hundred day-old chicks strain Cobb were divided into 4 groups of doses of Jamu jahkenkun administration (0.0, 0.1, 1.0 and 10.0 mL/L drinking water) each with 25 replications. Jamu jahkenkun was administered orally through drinking water. Administration of Jamu jahkenkun was started at the age of 8 days. Variables measured were growth performances including final body weight (BW), daily body weight gain (BWG), feed conversion ratio (FCR), mortality and water intake and erythrogram and leukogram. The results showed that broiler chickens administered with Jamu jahkenkun at doses of 0.1 and 1.0 mL/L for 4 weeks could optimize feed conversion so that increased final BW and daily BWG. Administration of Jamu jahkenkun at doses of 0.1 and 1.0 mL/L tended to give higher carcass and abdominal fat weights as compared to control. The hemogram data showed that the experimental broiler chickens were in normal physiological conditions without any abnormality and physiological disturbance. The experimental broiler chickens had higher number of erythrocytes and hemoglobin concentrations as compared to control on days 14 of Jamu jahkenkun administration. It was concluded that Jamu jahkenkun administration at a dose of 1.0 mL/L increased growth performance with normal hematogram parameters.
  Kasiyati , Sumiati , Damiana Rita Ekastuti and Wasmen Manalu
  Backgroud: The biosynthesis of vitellogenin as an egg-yolk precursor in oviparous animals is occurred in the liver cells. The vitellogenin synthetic capacity of hepatocyte is under the control of estrogen that its secretion increase when the oviparous animals reach sexual maturity. This experiment was designed to study the uses of curcumin and artificial monochromatic light on liver performance to improve vitellogenin synthesis to support egg production during laying period. Materials and Methods: One hundred and ninety two female magelang ducks were assigned into a completely randomized design with a 4×4 factorial arrangement. The first factor was dose of curcumin supplementation consisted of 4 levels i.e., 0, 9, 18 and 36 mg. The second factor was the color of monochromatic light consisted of 4 levels i.e., white, red, green and blue. Results: The results showed the interaction effects between curcumin and monochromatic light on the liver weight, hepatocytes diameter, DNA and RNA concentrations in the liver cells (p<0.05). Serum estradiol and vitellogenin concentrations in female magelang ducks during sexual maturity prior to laying period ranged from 0.06-0.19 and 0.36-3.46 mg mL–1, respectively. Curcumin supplementation at doses of 18 and 36 mg duck–1 day–1 increased the diameters of F1 follicles by 21.62 and 17.91%, respectively. Conclusion: It is concluded that curcumin supplementation at a dose of 18 mg duck–1 day–1 combined with the use of red or greenlight can improve the biosynthesis capacity of egg yolk protein precursors by liver cells without increasing liver cells proliferation. Moreover, the increased estradiol during vitellogenesis is also necessary in the recruitment of ovarian follicles in establishing the F1 hierarchical follicles.
  Andriyanto , Firda Agustin , Dedi Nur Aripin , Pramesti Nugraheni , Gusti Habibi Suryanata , Ridi Arif , Diah Nugrahani Pristihadi , Aulia Andi Mustika and Wasmen Manalu
  Background and Objective: Productivity and health performances of broiler chickens can be improved by using medicinal herb. An experiment was conducted to study the effect of administration of Jamu ginger or jahe (Zingiber officinale ), Jamu curcuma or kencur (Kaemferia galanga ) and Jamu turmeric or kunyit (Curcuma domestica ) on the productivity and health performances of broiler chickens. Materials and Methods: One hundred days old chicks of Cobb strain were used in the experiment. The experimental chicks were divided into 4 treatments, (1) Control group i.e., the broiler chicks without administration of Jamu, (2) The broiler chicks administered with Jamu ginger, (3) The broiler chicks administered with Jamu curcuma and (4) The broiler chicks administered with Jamu turmeric. Jamu preparations were administered orally when the experimental broiler reached the age of 8 days. Jamu treatment was administered for 28 days through drinking water at a dose of 1 mL L–1. Data were analyzed by using ANOVA and when the means were significant the data were further tested by using Duncan test. Results: The results showed that the experimental broiler chickens administered with Jamu curcuma and Jamu turmeric had improved feed conversion ratio by 19.52 and 14.76%, respectively, compared to control. The experimental broiler chickens administered with Jamu ginger had higher carcass weight (p<0.05) compared to those administered with Jamu curcuma, Jamu turmeric and control by 5.70, 61.10 and 8.21%, respectively. All experimental broiler chickens administered with Jamu had lower mortality rate compared to control. The administration of Jamu ginger, Jamu curcuma and Jamu turmeric decreased abdominal fat by 10.09, 5.05 and 28.08%, respectively, compared to control. The experimental broiler chickens administered with Jamu ginger, Jamu curcuma and Jamu turmeric had similar number of red blood cells and white blood cells, serum SGPT, SGOT and creatinine concentrations compared to control. Serum concentrations of urea in the experimental broiler chickens administered with Jamu turmeric were lower than the other treatments. Conclusion: It was concluded that the administration of Jamu ginger, Jamu curcuma and Jamu turmeric can be used as feed additives to increase the productivity and health performances of broiler chickens. Jamu ginger, Jamu curcuma and Jamu turmeric have a great potential to be used and developed as herbal preparations to improve the growth and performances of poultry animals.
  Sunarno , Wasmen Manalu , Nastiti Kusumorini and Dewi Ratih Agungpriyono
  Physiological aging and aging due to oxidative stress decrease alanine-glutamine dipeptide concentrations in plasma and hippocampus. These conditions impact the decreased availability of glutamine in intracellular and glutathione levels in the hippocampus. Decrease in hippocampal glutathione level will be followed by decreased function of hippocampal neuron. One way to improve the function of the hippocampus is to increase the availability of glutamic acid in hippocampal neuronal cells. This study was designed to obtain the concentration profile of alanine-glutamine dipeptide in plasma and hippocampus and the repair function in the aging hippocampus is known from structural repairs to the mitochondria of neurons. The experimental rats were assigned into a completely randomized design with 2 x 2 x 2 factorial arrangement and three replications. The first factor was the age of the experimental rats, consisted of two levels i.e., 12 and 24 months. The second factor was oxidative stress consisted of two levels, i.e., without or with oxidative stress. The third factor was the concentration of alanine-glutamine dipeptide administration consisted of 2 concentrations, i.e., 0 and 7%. Administration of alanine-glutamine dipeptide 7% can improve function in the aging hippocampus, both in physiological aging or aging due to oxidative stress in younger or aged rats, in normal or oxidative stress rats. This research concluded that the alanine-glutamine dipeptide 7% gave the best results in increased alanine-glutamine dipeptide plasma and hippocampus, glutathione levels, the repair response of mitochondrial structure that mediates the repair function in the aging hippocampus.
  Ronald Tarigan , Nastiti Kusumorini and Wasmen Manalu
  Obesity has been a world-wide health problem and associated with many degenerative diseases. Two approved anti-obesity compounds, sibutramine and orlistat, have various serious side effects which limit their uses. IgY anti lipase will inhibit the activity of pancreatic lipase in the gastrointestinal tract. The objective of this study was to evaluate the effectivity of IgY anti lipase obtained from yolk as pancreatic lipase inhibitor for prevention of obesity. IgY anti lipase was obtained from the yolk of hens immunized with porcine pancreatic lipase and was purified with sodium sulphate precipitation. The protein was analyzed by SDS-PAGE and was identified by Immunoblotting. The ability of IgY anti lipase to inhibit pancreatic lipase was tested by ELISA colorimetric microplate assay at five grades of concentration. Yolk containing IgY anti lipase was then tested in rabbit to evaluate its ability to inhibit fat absorption in vivo. The result showed that IgY contained two proteins with molecular weights of 61.2 and 26.9 kDa and was positively reacted with the rabbit anti chicken coupled with HRP. IgY anti lipase and orlistat did not differ in inhibition capacity at various concentrations (p>0.05). The inhibition capacity of IgY obtained from unimmunized hen was significantly lower than IgY anti lipase and orlistat (p<0.05). Yolk containing IgY anti lipase also had the ability to inhibit dietary fat absorption in vivo, but its inhibition capacity was lower than orlistat (p<0.05). It is concluded that IgY anti lipase could be used to inhibit fat absorption to prevent obesity.
  Ronald Tarigan , Nastiti Kusumorini and Wasmen Manalu
  Obesity has been a world-wide health problem and associated with many degenerative diseases. Two approved anti-obesity compounds, sibutramine and orlistat, have various serious side effects which limit their uses. IgY anti lipase will inhibit the activity of pancreatic lipase in the gastrointestinal tract. The objective of this study was to evaluate the effectivity of IgY anti lipase obtained from yolk as pancreatic lipase inhibitor for prevention of obesity. IgY anti lipase was obtained from the yolk of hens immunized with porcine pancreatic lipase and was purified with sodium sulphate precipitation. The protein was analyzed by SDS-PAGE and was identified by Immunoblotting. The ability of IgY anti lipase to inhibit pancreatic lipase was tested by ELISA colorimetric microplate assay at five grades of concentration. Yolk containing IgY anti lipase was then tested in rabbit to evaluate its ability to inhibit fat absorption in vivo. The result showed that IgY contained two proteins with molecular weights of 61.2 and 26.9 kDa and was positively reacted with the rabbit anti chicken coupled with HRP. IgY anti lipase and orlistat did not differ in inhibition capacity at various concentrations (p>0.05). The inhibition capacity of IgY obtained from unimmunized hen was significantly lower than IgY anti lipase and orlistat (p<0.05). Yolk containing IgY anti lipase also had the ability to inhibit dietary fat absorption in vivo, but its inhibition capacity was lower than orlistat (p<0.05). It is concluded that IgY anti lipase could be used to inhibit fat absorption to prevent obesity.
  Jola J.M.R. Londok , Wasmen Manalu , Komang G. Wiryawan and Sumiati
  Background: Besides as an energy source, coconut oil with its lauric acid content can improve the growth performance, carcass characteristics and fatty acids profile of broiler chickens. Conventional processing of coconut oil is susceptible to hydrolytic oxidation that reduces its antioxidant content. Areca vestiaria Giseke (AV) with its phenol content acts as a natural antioxidant in the diet. Materials and Methods: Two hundred and forty day-old unsexed Lohmann broiler chicks (MB-202 P) were divided into 24 experimental units (ten chicks/unit) and arranged in a completely randomized design with a 2×4 factorial arrangement. Each experimental unit was repeated 3 times each with ten chicks. The first factor was the source of lauric acid in the ration consisted of 2 levels i.e., coconut oil and pure lauric acid. The second factor was dose of antioxidant consisted of 4 levels i.e., 0 [without antioxidant (AV and lauric acid) supplementation], AV at a dose of 625 mg kg–1 ration, AV at a dose of 1250 mg kg–1 ration and tocopherol at a dose of 200 ppm). Parameters measured were growth performance, carcass characteristics and fatty acid profiles of broiler breast meat. Results: On the first stage trial, AV can be used as a source of natural antioxidant in the diet of broiler. The feeding trial showed that the treatments highly significantly affected (p<0.01) weight gain, feed consumption, feed conversion ratio, breast weight/eviscerated weight percentage, abdominal fat weight/eviscerated weight percentage and significantly affected (p<0.05) dressing percentage. Low growth performance and carcass characteristics in broiler chickens supplemented with vitamin E were assumed to be caused by the inhibition of absorption. Fatty acids in feed after consumption will be relatively unchanged in body tissue. Lauric acid can be deposited in breast meat. Conclusion: AV as a source of natural antioxidant can be used as a supplement in broiler ration containing coconut oil as a source of lauric acid.
 
 
 
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