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Articles by Waseem Ahmad Butt
Total Records ( 2 ) for Waseem Ahmad Butt
  Ayesha Khan , Ikram-ul-Haq , Waseem Ahmad Butt and Sikander Ali
  Present investigation deals with the isolation and screening of Aspergillus niger isolates for the biosynthesis of xylanase. About 104 different strains of Aspergillus niger were isolated from soil collected from different areas of Lahore by serial dilution method on xylene agar malt extract medium. The strains with larger whitish zones of xylene hydrolysis were picked up and transferred to the PDA slants. The cultures were incubated at 30°C for 3-5 days for maximal sporulation. The Aspergillus niger strains were screened for xylanase production by submerged fermentation. Xylanase activity was ranged from 30-225 U ml-1 and dry cell mass from 6.48-26.56 g L-1. Maximum xylanase productivity was obtained by GCBT-35 (225 U ml-1). The mycelial dry weight was 9.54 g L-1 while mycelia were small in size and rounded in shape.
  Ikram-ul-Haq , Ayesha Khan , Waseem Ahmad Butt , Sikander Ali and M. A. Qadeer
  The present study is concerned with the effect of carbon and nitrogen sources and their concentrations on the production of xylanase by mutant strain of Aspergillus niger GCBMX-45. High xylanase activities (2350 U/g) were observed when starch (2%) was used as carbon source. (NH4)2SO4 (0.2%) was found to be best nitrogen source for optimum enzyme production i.e., 2480 U/g. The production of enzyme reached maximum, 72 hours after inoculation. All the fermentations were carried out at 30°C at 0.4 cm (10g wheat bran) depth of the basal medium.
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