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Articles by Waqar Ahmad
Total Records ( 4 ) for Waqar Ahmad
  Taous Khan , Mansoor Ahmad , Hamayun Khan and Waqar Ahmad
  The elemental analysis of crude extracts derived from selected medicinal plants of Pakistan, including Trichodesma indicum (whole plant), Paeonia emodi (aerial parts), Aconitum laeve (roots) and Sauromatum guttatum (corms, berries and leaves) was carried out using SEM-EDX technique. Various elements such as C, O, Cl and K were present in all tested plants` extracts, Al was observed in P. emodi and A. laeve, S was detected in T. indicum and S. guttatum (corms and leaves) while Mg and P were found only in S. guttatum (corms). The data showed that C was present in highest quantity in T. indicum and S. guttatum (leaves) (>80%) followed by O while in the rest of extracts these two elements were found in approximately equal quantities. The other detected elements were present in comparatively trace amounts. These results were also evaluated in correlations with previously reported biological activities of the tested extracts. This study would provide a new and alterative technique for the standardizations of crude extracts.
  Zahida Parveen , Yulin Deng , Muhammad Khalid Saeed , Rongji Dai , Waqar Ahmad , Yuhong Yu and Tahmina Yasmeen
  Astragalin, one of the flavonoid extracted from plants is increasingly in demand in food and pharmaceutical industries due to its various biological and physiological activities including antioxidation, antiallergy, anti-inflammation and anti-hepatoptoxic. The whole plant of Thesium chinense Turcz is a major source of astragalin. This study developed a low-cost process encompassing the efficient extraction, fractionation, isolation and recrystallization to obtain high-purity astragalin from Thesium chinense and it could improve the economic utilization of this plant. In this study plant was extracted with 80% ethanol for 3 h and extract was fractionated on AB-8 (styrene based copolymer) with water, 20, 40 and 80% aqueous ethanol. Astragalin was isolated by silica gel column and purified by recrystalization with methanol. These conditions resulted 90% recovery of Astragalin with 95% purity.
  Waqar Ahmad , Bashir Ahmad , Manzoor Ahmad , Zafar Iqbal , Muhammad Nisar and Mansoor Ahmad
  The 80% methanolic extract of Myricaria elegans Royle was investigated for in vitro acetylcholinesterase (ACHE), butyrylcholinesterase (BCHE) and lipoxygenase enzyme inhibition activities. The crude extract was found to have significant acetylcholinesterase inhibitory activity (74.8%) and remarkable butryrylcholinesterase inhibitory activity (96.0%). However, no activity was observed against the enzyme lipoxygenase (0.0%).
  Taous Khan , Waqar Ahmad , Shumaila Bashir , Zafar Iqbal , Bashir Ahmad Manzoor Ahmad , Nisarullah , Muhammad Arfan and Farzana Shaheen
  The seed oil of Abroma augusta Linn. was studied for biological and pharmacological properties. The oil was also screened for various in vitro biological and pharmacological activities including antifungal, antibacterial, insecticidal, phytotoxic and brine-shrimp cytotoxic activities. The oil exhibited remarkable phytotoxic activity against Lemna aeguinoctailis Welve. It was also explored to posses moderate antifungal activities against Trichophyton schoenleinii (56%) (human pathogens) and Microsporum canis (50%) (animal pathogen). The Abroma augusta seed oil did not shows the significant antibacterial, insecticidal activities and Brine shrimp cytotoxicity.
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