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by
Wannee Jiraungkoorskul |
Total Records (
7 ) for
Wannee Jiraungkoorskul |
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Kandarat Lamchumchang
,
Wannee Jiraungkoorskul
,
Somphong Sahaphong
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Tawewan Tansatit
,
Piya Kosai
and
Raviporn Madarasmi
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This study investigated the influence of dietary calcium supplementation to reduce the toxicity of sublethal lead concentration in Nile tilapia with emphasis on biochemical and histopathological analysis. The values of 24, 48, 72 and 96 h LC50 of lead to tilapia were 247.51, 197.47, 193.36 and 182.38 mg L-1, respectively. Therefore, lead concentration tested in the sublethal experiment was 45 mg L-1, which correspond to 25% of the 96 h LC50. Fish were fed with 0, 20 and 60 mg Ca2+ g-1 food for 30 days. In the gills of Pb treatment, edema, lamellar cell hyperplasia, epithelial lifting, lamellar fusion and aneurysm were observed. In the liver, there were blood congestion in sinusoids, vacuolation of hepatocytes and necrosis. In the kidney, glomerulus’s atrophy, tubular swelling and also necrosis were seen. The structural damages could be correlated to the significant increase (p≤0.05) in aminotransferase activities. Fish fed with Ca2+ supplemented diets showed slightly alteration when compare the only Pb treatment groups. These results indicated that dietary Ca2+ will be protective in reducing Pb burdens in fish exposed to environments contaminated with waterborne Pb. |
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Manop Kjanijou
,
Kanitta Jiraungkoorskul
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Piya Kosai
and
Wannee Jiraungkoorskul
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The mosquito-borne diseases are still the public health’s problems. So, in order to cope with these problems the development of ideal and novel natural product is essential for mosquito larvae’s control. The efficacy of mosquito larvicide of Murraya paniculata leaf aqueous extract was investigated by determining the median lethal concentration, LC50, within 24, 48, 72 and 96 h. Additional, the 25% 24 h LC50 of this extract against the Culex quinquefasciatus larva was investigated after 24 h exposure by using the histological analysis. The histology of the mid-gut was studied and it was found to be different from the normal larvae such as separation of the epithelial cells from the basement membrane, elongation protruded into its lumen, disruption of the microvilli, appearance several vesicles and cytoplasm masses. The results of this study suggested that the aqueous extract of this leaf had a larvicidal property and it could a used as a novel way to control and eradicate the mosquito’s larvae, without the use of harmful pesticides currently available in the market. |
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Wannee Jiraungkoorskul
and
Somphong Sahaphong
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This study was investigated the effects of copper (Cu) and Ascorbic Acid (AA) on butterfish (Poronotus triacanthus), using the micronucleus (MN) and Nuclear Abnormality (NA) tests for the period of 7, 14 and 21 days. NA shapes in erythrocytes were scored into blebbed nuclei (BL), lobed nuclei (LB), notched nuclei (NT) and binuclei (BN). It was observed that, fish showed significant sensitivity to the different treatments. In general, the highest value of both MN and NA cells were significantly increased in the Cu treated group followed by the combination of Cu and AA treated group. These values revealed the highest number after 21 days treatment in all cases. The frequencies of each NA shape in erythrocytes of all treatments were observed in the following NT> LB> BN> BL. Present results demonstrated the efficacy of ascorbic acid in reducing genotoxicity in fish induced by waterborne copper. |
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Wannee Jiraungkoorskul
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Somphong Sahaphong
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Niwat Kangwanrangsan
and
Myung-Huk Kim
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The effects of cadmium and ascorbic acid on the red-tailed tinfoil barb (Puntius altus) were compared using light microscopic study for the period 24, 48, 72 and 96 h. In the gills, edema, lamellar cell hyperplasia, epithelial lifting and aneurysm were observed. In the liver, there were blood congestion in sinusoids, vacuolation of hepatocytes, hemosiderin accumulation, apoptosis and nuclear pyknosis. In the kidney, glomerulus atrophy and apoptotic cells were seen. In tubular cells, there were hydropic swelling, hyaline casts, apotosis and necrosis. Their changes occurred predominantly in the 48 h treatment. In the combination of Cd and ascorbic acid treated group, they showed similar alterations as those observed in the Cd treated alone group but they were less severe. The findings of this study can be used as guidelines for developing programs to help the fish, which are cultured near the cadmium contaminated areas. |
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Wannee Jiraungkoorskul
,
Piya Kosai
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Somphong Sahaphong
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Pukaew Kirtputra
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Jasmin Chawlab
and
Shanida Charucharoen
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Nile tilapia (Oreochromis niloticus), Butterfish (Poronotus triacanthus) and Red-tailed tinfoil barb (Puntius altus), three fish species inhabiting in Southeast Asia freshwater ecosystems were evaluated for their use as pollution biomarkers using the micronucleus (MN) and nuclear abnormalities(NA) tests in erythrocytes. NA shapes were scored into blebbed nuclei (BL), lobed nuclei (LB), notched nuclei (NT) and binuclei (BN). Fish were exposed to lead (Pb), copper (Cu) and cadmium (Cd) for the period of 24, 48, 72 and 96 h. It was observed that, fish species showed significant sensitivity to the different heavy metals treatment. In general, the highest value of both MN and NA cells were significantly increased in the exposure to Pb followed by Cu and Cd. On the other hand, Nile tilapia was the most sensitive to the three heavy metals exposure. The frequencies of each NA shape were found in the all fish species and in the all treatments as following NT>LB>BN>BL. Results of MN and NA revealed the highest number after 48 h treatment in all cases and decreased within the longer time exposure. Our results demonstrated the suitability of Nile tilapia for genotoxicity of pollution biomarkers using the micronucleus and nuclear abnormalitiestest. |
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Wannee Jiraungkoorskul
,
Rachen Singhakumar
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Kanitta Jiraungkoorskul
and
Piya Kosai
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The efficiency of Psidium guajava was studied to illustrate the reduction of ferric nitrilotriacetate (Fe-NTA) toxicity in Puntius altus via the histopathology analysis. The fish (n = 40) were randomly divided into four groups. Each fish was transferred to each aquarium as follows: G1 and G2 were obtained normal fish food; G3 and G4 were obtained guava leaf extract 60 mg g-1 fish food. After 28 days dietary supplement, fish in G2 and G4 were injected intraperitoneal of 9 mg Fe kg-1 b.wt. Twenty-four hour after injection, lesions were especially most evident in the G2. The gills were observed epithelial lifting, lamellar cell hyperplasia. Blood congestion was seen in sinusoids and hepatocytes necroses were also observed. Renal tubular swelling and necrosis were seen. Some areas were found hemosiderin pigment accumulation. Fish with guava pre-treatment (G4) showed slightly alteration when compare those of G2 group. The results suggested that P. guajava leaf extract pre-obtained may play an important role in the reduction of Fe-NTA toxicity in fish. |
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