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Articles by Wang Jin-yu
Total Records ( 6 ) for Wang Jin-yu
  Mekki Dafaalla Mohammed , Yousif Ibrahimm Abdalsalam , Abdelrahman Mohammed Kheir , Wang Jin-yu and Musa Hassan Hussein
  Three local types of Sudanese indigenous fowls, large Baladi (LB), Bare-neck (BN) and Betwil (BT) were studied for detection of maturity live weight and egg characteristics. The (BN) average live weight (1547.2±274.5 gm) was heavier than either (LB) (1494.4±349.8 gm) or (BT) (1198.3±257.5 gm), The Betwil average live weight is significantly (P< 0.05) lighter than those of other two local types. The weekly hen-day egg production means were 3.7, 3.2 and 3.9 for (BN), (BT) and (LB) respectively, while the corresponding hen-housed egg production means were 3.3, 2.7 and 3.4. The rate of egg production during the laying period (36 weeks) was 47.14, 38.57 and 48.57 for (BN), (BT) and (LB) respectively. There were significant differences (P< 0.05) in average egg-shell thickness among local types. The means of egg-shell thickness for (BT) and (BN), 36.2±4.2 and 36.2±4.0μrespectively were similar and both significantly thicker than that of (LB), 34.3±3.6μ
  Mekki Dafaalla Mohammed , Yousif Ibrahimm Abdalsalam , Abdel Rahman Mohammed kheir , Wang Jin-yu and Musa Hassan Hussein
  Two thousand three hundred and fourty six chickens, line X tester crosses were obtained from fifteen consecutive hatches at weekly interval. Testers were exotic cockerels; Rhode Island Red (RIR), Bovans (BO) and Egyptian Fayoumi (FO), while lines were indigenous hens; large Baladi (LB), Bare-neck (BN) and Betwil (BT). The nine genetic groups of crosses were reared up to 18 weeks of age in litter opened-house system. Significant differences (DMRT 5%) for average body weight of different crosses were obtained at hatching, 2, 14, 16 and 18 weeks of age. Biweekly average weight gain showed similarity in growth pattern of the various crosses. Sex affected body weight insignificant at hatching, whereas the differences were significant (P< 0.05) at 2 weeks of age and highly significant (P< 0.01) for the subsequent ages. Hatching effect was found to be highly significant (P< 0.01) on body weight at various ages; however, sex X hatch interaction was found to be significant (P< 0.05) at day old and disappeared thereafter. The average live weight at 18 week of age for the nine groups was adjusted for hatching and sex effects. There were significant differences (P< 0.05) of lines and testers, however, line X tester interaction was not significant for 18 weeks body weight. The estimated general combining ability (gca), thus the additive gene effect, was relatively high for both lines (-42.03, 19.90 and 22.13) and testers (-36.74, 10.74 and 26.00). On the other hand the specific combining ability (sca), which involves dominance, over dominance and epistasis effects, was found to be minor in both positive and negative values for the nine groups, ranging from -14.66 to 17.37. The general combining ability estimated was of high value and seemed to be much important than specific combining ability for body weight at 18 weeks of age.
  Yu Ya-Bo , Wang Jin-Yu , D.M. Mekki , Tang Qing-Ping , Li Hui-Fang , Gu Rong , Ge Qing-Lian , Zhu Wen-Qi and Chen Kuan-Wei
  A total of 720 individuals of 12 indigenous chicken populations, geographically localized in Southern China were genotyped for 30 microsatellite markers in polymerase chain reaction (PCR) to evaluate the genetic variation and genetic distance between populations. All microsatellites were found to be polymorphic. Heterozygosity was calculated to determine the genetic variation. Of the 30 microsatellite loci, number of alleles per locus (Na) and effective number of alleles per locus (Ne) ranged from 4 to 11 and 2.157 to 8.019, respectively. The average expected heterozygosity (HE) was 0.669, while the average observed heterozygosity (HO) was 0.764. The polymorphism information content (PIC) has values between 0.560 and 0.641. Using Nei`s standard distance, genetic distance (DA) calculated ranged between 0.088 (Guanxi Sanhuang vs. Nandan Yao) and 0.495 (Huiyang Beard vs. Zhangzhou Game). The topology of phylogenetic trees constructed showed general patterns of relationship and genetic differentiation among the indigenous populations studied, however, both trees from Neighbor-Joining method and Unweighted Pair Group method showed a similar topology. The results provided evidence of the applicability of microsatellite to determining the genetic relatedness among different Chinese indigenous chicken populations and evaluating of genetic variations.
  Dafaalla M. Mekki , Bian Liang-yong , Wang Jin-yu , Yang Yan , Li lin-chuan , Yu Yabo and Hassan H. Musa
  Semi-quantitative polymerase chain reaction (RT-PCR) was applied to quantify and compare variations in avian E2A expression level between two Chinese indigenous duck breeds for different tissues. Six primer combinations in addition to β-actin as an internal control were used. The expression level of E2A was significantly higher (p< 0.05) in kidney tissues than lung tissues for both Gao you and Kun shan indigenous duck breeds. The variability of gene expression between two breeds showed 34.35% and 60.70% higher expression in Gao you breed than Kun shan breed for kidney and lung tissues, respectively. Differences were found to be significant (p< 0.05) only for expression level in lung tissues. No significant effect of sex upon E2A expression was detected.
  Dafaalla M. Mekki , Bian Liang-yong , Wang Jin-yu , Yang Yan , Yu Yabo and Hassan H. Musa
  Semi-quantitative polymerase chain reaction (RT-PCR) was applied to quantify and compare variations in avian E2A expression level between two Chinese indigenous duck breeds for different tissues. Six primer combinations in addition to beta-actin as an internal control were used. The expression level of E2A was significantly (p< 0.05) higher in kidney tissues than lung tissues for both Gao you and Kun shan indigenous duck breeds. The variability of gene expression between two breeds showed 34.35% and 60.70% higher expression in Gao you breed than Kun shan breed for kidney and lung tissues, respectively. Differences were found to be significant (p< 0.05) only for expression level in lung tissues. No significant effect of sex upon E2A expression was detected. The association between E2A relative expression level and body weight was found to be positive and moderate for the genetically lean breed (Gao you), while it was negative and has low values for genetically heavy breed (Kun shan).
  Wang Jin-yu , Bian Liang-yong , Yang Yan , Li lin-Chuan , H. Hassan Musa and M. Dafalla Mekki
  Semi-Quantitative Polymerase Chain Reaction (RT-PCR) was applied to quantify and compare variations in avian TCF3 expression level between two Chinese indigenous chicken breeds for different tissues. Six primer combinations in addition to -actin as an internal control were used. The expression level of TCF3 was greater in Jinghai breed than Suqin breed for different tissues. The variability of gene expression between two breeds showed 18.55, 37.85 and 66.15% higher expression in Jinghai breed than Suqin breed for kidney, lung and spleen tissues, respectively. Differences were found to be significant (p<0.05) only for expression level in lung and spleen tissues. Significant effect of sex upon TCF3 expression was detected for both breeds.
 
 
 
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