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Articles by Wan Mohtar Wan Yusoff
Total Records ( 10 ) for Wan Mohtar Wan Yusoff
  Hayyan Ismaeil Al-Taweil , Mohammad Bin Osman , Aidil Abdul Hamid and Wan Mohtar Wan Yusoff
  Problem statement: The present study was undertaken to assess the Empty Fruit Bunches (EFB) as carrier of Biofertilizer and microbial inoculants interaction. A study in Malaysia had shown a strain of Trichoderma viride and Bacillus megaterium were isolated from the soil. Questions were raised whether the tow inoculants compatible to be mixed in same carrier? It is important to develop delivery system of the inoculants. The present study was undertaken to assess the Empty Fruit Bunches (EFB) as carrier of biofertilizers and microbial inoculants interaction and the impact on rice seedlings.
Bacillus negaterium and Trichoderma viride were grown in their respective broth culture. Cells numbers in broth pr suspension were determined. EFB powder 200 g in polypropylene bags was sterilized. Inoculums from broth of suspension were added in EFB using sterilized syringe, moisture level in each packet was maintained by adding respective broth, 1 g was periodically withdraw from fomenters, soaked in 10 mL sterilized distal water, then serially diluted and plated out, the number of viable cells was expressed as cfu g-1 dry root. Twenty one-day-old rice seedlings of MR220 cultivar were grown under greenhouse conditions in autoclaved homogeneous soil, rice seedlings were treated with Trichoderma and Bacillus. Five replicates were used for each treatment. Rice seedlings height (cm), root length (cm), root numbers, root and shoot dray matter, parameters were measured during and after experiment. All data were analyzed at an alpha level of 0.05 or 0.10 using experimental design by completely randomized design model using the analysis of variance module (treatments x 3 R) all treatment means were separated using Fisher’s protected least significant difference (LSD) mean separation.
Trichoderma growth in carrier reduces Bacillus growth as interaction effect of both in same carrier which indicated that Bacillus unable to develop on multi-inoculants biofertilizers with Trichoderma. Inoculation of rice seedlings with Bacillus megaterium and Trichoderma viride produced maximum shoots and roots dry matter, increased significantly root length numbers and seedlings height comparing with control and same action of NPK fertilizers.
The present study showed the possibility to develop the delivery system of biofertilizers which contained Bacillus and Trichoderma as mono inoculums not as multi-inoculants due to incompatible of both in same carrier. Bacillus and Trichoderma also showed appositive effect with rice seedlings.
  Mohd Sahaid Kalil , Hisham Salem Alshiyab and Wan Mohtar Wan Yusoff
  Problem statement: One of the main factors influenced the bacterial productivity and total yield of hydrogen is the nitrogen source and its concentration. Approach: Using different nitrogen source with different concentration on bacterial productivity of hydrogen showed to affect on both bacterial productivity of hydrogen and biomass concentration. Results: Yeast extract as nitrogen source at concentration of 13 g L-1 was the best organic nitrogen source and resulted in hydrogen yield YP/S of 308 mL g-1 glucose utilized with biomass concentration of 1.1 g L-1, hydrogen yield per biomass YP/X of 280 mL g-1 L-1, biomass per substrate utilized YX/S of 0.22 and produced hydrogen in gram per gram of glucose utilized YH2/S of 0.0275. C/N of 70 enhanced the YP/S from 308-350 mL g-1 glucose utilized with biomass concentration of 1.22 gL-1, YP/X of 287 mL g-1 L-1, YX/S of 0.244 and YH2/S of 0.03125. Conclusion: Nitrogen source with proper C:N ratio enhanced the hydrogen production.
  Hisham Salem Alshiyab , Mohd Sahaid Kalil , Aidil Abdul Hamid and Wan Mohtar Wan Yusoff
  Problem statement: One of the main factors influenced the bacterial productivity and total yield of hydrogen is the partial pressure of produced gas. A novel solution to enhance the bacterial productivity was through reduction of gas pressure. Approach: Increasing the reactor size showed to enhance the bacterial production of hydrogen. Results: The technique of increasing reactor size resulted to enhance the hydrogen yield (YP/S) from 269 mL g-1 glucose utilized to maximum yield of 448 mL g-1 glucose utilized by using 125 mL and 2 L reactor size respectively. The hydrogen productivity was also enhanced from 71 mL-1 h-1 to maximum of 91 mL L-1 h-1 was obtained by using 125 mL and 1 L reactor size respectively. Biomass concentration was enhanced from 1.03 g L-1 to maximum of 1.68 g L-1 by using 125 mL and 2 L reactor size were used respectively, hydrogen yield per biomass (YP/X) of 267 mL g-1 L-1, biomass per substrate utilized (YX/S) of 0.336 and produced hydrogen in gram per gram of glucose utilized (YH2/s) of 0.04 when 2 L reactor size was employed. Conclusion: By using bigger reactor size, the effect of gaseous products in fermentation medium was reduced and enhanced both bacterial productivity and biomass concentration.
  Febri Doni , I. Anizan , C.M.Z. Che Radziah , Wan Natasya Wan Ahmed , Abidah Ashari , Eka Suryadi and Wan Mohtar Wan Yusoff
  Local isolates Clostridium sp. FWM1 and Pseudomonas sp. FA1 were studied with respect to their growth promoting effect on rice seedlings using a completely randomized design under greenhouse condition. Seedling growth components including rice seedling height, root length, wet weight, leaf number and biomass were used as parameters for this study. The results showed that Clostridium sp. FWM1 and Pseudomonas sp. FA1 significantly enhanced rice seedling wet weight and biomass. This suggests that Clostridium sp. and Pseudomonas sp. deserve to be considered as potential rhizobacterial inoculants for sustainable rice production.
  El Mubarak Musa Tibin Musa , Najeeb Kaid Nasser Al- Shorgani , Nawal Noureldaim Abuelhassan , Febri Doni , Wedad Hassan Abdelhaleem , Aidil Abdul Hamid , Mohd Sahaid Kalil and Wan Mohtar Wan Yusoff
  The optimum conditions for cellulase activity measured as Filter paper activity (FPase) from sorghum straw by Aspergillus terreus SUK-1 using Response Surface Methodology (RSM) was based on Central Composite Design (CCD). The effect of incubation temperature, initial pH and moisture content on FPase activity was carried out in Solid Substrate Fermentation (SSF). The results were analysed by analysis of variance (ANOVA) and the regression quadratic model was obtained. ANOVA analysis indicated that the model was significant (p<0.05) and that the effect of temperature and moisture content significantly effected on the FPase activity. The optimized conditions are; temperature 25°C, pH 4 and moisture content of 40%, while the predicted FPase activity is 0.35 U mL‾1. The model was validated by applying the optimized conditions and it was found that the FPase activity was 0.36 U mL‾1 which indicate the validity of the model.
  Jahwarhar Izuan Abdul Rashid , Noraini Samat and Wan Mohtar Wan Yusoff
  Optimization of three parameters, temperature (25-35°C), moisture content (40% (w/v)-60% (w/v) and inoculum sizes (5% (w/v)-15% (w/v) were investigated and optimized by Response Surface Methodology (RSM) for optimal mannanase production by Aspergillus terreus SUK-1. A second order polynomial equation was fitted and the optimum condition was established. The result showed that the moisture content was a critical factor in terms of its effect on mannanase. The optimum condition for mannanase production was predicted at 42.86% (w/v) initial moisture (31°C) temperature and 5.5% (w/v) inoculum size . The predicted optimal parameter were tested in the laboratory and the mannanase activity 45.12 IU mL-1 were recorded to be closed to the predicted value (44.80 IU mL-1). Under the optimized SSF condition (31°C, 42.86% moisture content (w/v) and 5.5% inoculum size (w/v)), the maximum mannanase production was to prevail about 45.12 IU mL-1 compare to before optimized (30°C, 50% moisture content (w/v) and 10% inoculum size (w/v)) was only 34.42 IU mL-1.
  Jahwarhar Izuan Abd Rashid , Noraini Samat and Wan Mohtar Wan Yusoff
  Microbial mannanases have become biotechnologically important in industry but their application is limited due to high production cost. In presents study, the extraction of mannanase from fermented Palm Kernel Cake (PKC) in the Solid State Fermentation (SSF) was optimized. Local isolate of Aspergillus terreus SUK-1 was grown on PKC in (SSF) using column bioreactor. The optimum condition were achieved after two washes of fermented PKC by adding of 10% glycerol (v/v) soaked for 10 h at the room temperature with solvent to ratio, 1:5 (w/v).
  Emran Issa Said Khamaiseh , Aidil Abd. Hamid , Wan Mohtar Wan Yusoff and Mohd Sahaid Kalil
  Date fruit juice contains high concentration of simple sugars ranging from 65 to 75% (w/w) in dry form. In this study, the potential of date fruit juice as biobutanol fermentation medium by C. acetobutylicum was investigated. The fermentation process was carried out at initial pH of 5, 6 and 7, incubation temperature of 30, 35 and 40°C for 72 hours. The date fruit concentrations tested were 10, 20, 30 and 40 g L-1. Medium containing 30 g L-1 of date fruit at 35°C incubation temperature with initial medium pH 7.0 gave the highest concentration of solvents of 3.1, 0.1 and 1.1 g L-1 butanol, ethanol and acetone respectively. The yield and productivity of biobutanol were 0.32 g g-1 and 0.044 g L-1/h respectively, while for total ABE were 0.45 g g-1 and 0.06 g L-1 h, respectively.
  Wan Mohtar Wan Yusoff , Muhannad I. Massadeh , Othman Omar and Jalil Kader
  Trichoderma reesei QM9414 was co-cultured with Aspergillus terreus SUK-1 for cellulase and reducing sugar production on sugar cane bagasse in solid substrate fermentation. The highest cellulase activity (FPase 0.68 (IU/ml), β-glucosidase activity (0.08 IU/ml) and reducing sugar concentration (1.4 mg/ml) were obtained in mixed cultures using inoculum ratio of 2:1 (Trichoderma : Aspergillus) compared to other ratios investigated (1:2 and 1:1). Nevertheless, experiments with pure culture of T. reesei was found to be better in terms of the production of FPase (1.4 IU/ml) and reducing sugar concentration (2.3 mg/ml), The addition of A. terreus to T. reesei in cultures of 2:1 inoculum ratio increases the production of β-glucosidase (the highest activity of 0.08 lUlml was detected in cultures of 2:1 ratio as well as in pure cultures of A. terreus). Nevertheless, this enhanced (β-glucosidase activity in mix culture could not result in enhanced substrate utilization and reducing sugar production. It was found that the highest percentage of degradation (25%) was achieved when pure cultures of T. reesei was employed followed by when pure cultures of A. terreus was employed (22%) and when mix cultures of 2:1 inoculum ratio was employed (22%). This suggests that synergism of β-glucosidase and cellulase to enhance substrate degradation was better affected using pure culture than mixed culture in solid substrate fermentation of sugar cane bagasse.
  Che Husna Azhari , Aulia Saad , Wan Mohtar Wan Yusoff and A. Ikram
  The degradation of natural rubber in a continuous culture system using actinomycete has been investigated at three exposure times (30, 60 and 90 days) employing diagnostic matching as well as the evaluation of mechanical and chemical properties. The biodegradation was carried out in a packed bioreactor varying three operating parameters; temperature (20°C, 30°C, 40°C and 50°C) pH (5,6,7 and 8) and aeration rate (0, 0.3, 0.5 and 1 vvm). The actinomycete growth characteristics were also investigated and found to be the best in a minimal media with a concentration of 0.1 g/l glucose. The actinomycete was able to grow in both glucose and non-glucose environments. In diagnostic matching, the morphology of degradation was matched with actual conditions using scanning electron microscopy. The morphologies observed were; striations, asperities, voids and bulk degradation. The intensity of biodegradation runs parallel with morphology in the order thus; striations, asperities, voids and bulk degradation. Asperities were observed at 60 days and temperatures of 20-40°C, striations at 40°C and an exposure time of 60 days. The morphologies with change in rate of aeration were voids and bulk disintegration. Voids were observed for all samples at aeration rates of 0.3-1vvm with onset at 30 days exposure. The morphology of degradation for the change in pH was voids with onset at 30 days exposure time. The voids then grew with increase in pH as well as exposure times, but did not manifest as bulk degradation at the end of the exposure, at the higher temperatures. The morphology of degradation was matched to the mechanism, enabling a mapping of the process to be made.
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