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Articles by Wahyu Widowati
Total Records ( 4 ) for Wahyu Widowati
  Wahyu Widowati , Laura Wijaya , Dwi Agustina , Harry Murti , Nurul Fauziah , Sutiman B. Sumitro , M. Aris Widodo and Indra Bachtiar
  Cancer is one of the leading causes of mortality and morbidity throughout the world. Since there are still some problems related to the conventional therapies for cancer treatment, it is critical to explore new more efficient therapy strategies. Mesenchymal Stem Cells (MSCs) are one of powerful tools for tissue engineering for regenerative medicine, as recent research aims to utilize MSCs for anti-cancer treatment. Our previous research demonstrated that Conditioned Medium from Whartons’ Jelly MSCs (WJ-MSCs-CM) significantly lowered cancer proliferation of various cancer cell lines. This research was performed to evaluate the tumoricidal property of cell lysate from WJ-MSCs from normoxia (WJMSCs-norCL) and hypoxia-treated WJMSCs (WJMSCs-hypoCL) on the proliferation of human cancer cells, including cervical (HeLa), liver (HepG2), ovarian (SKOV3) and oral squamous (HSC3) cancer cell lines compared to normal cells including mouse fibroblast (NIH3T3), human Mesenchymal Stem Cells (hMSCs), human fibroblast. The WJMSCs-norCL and WJMSCs-hypoCL have cytotoxic activity, reduce proliferation of various cancer cell lines with minimum inhibitory concentration (IC50) 21.094-95.928 μg mL–1 and no cytotoxic to normal cells with IC50 409, 191-629, 799.738 μg mL–1. The WJMSCs-norCL and WJMSCs-hypoCL inhibit proliferation in various cancer cell lines and are not toxic for normal cells.
  Djaja Rusmana , Mariska Elisabeth , Wahyu Widowati , Nurul Fauziah and Maesaroh Maesaroh
  Various plants and their derived compounds have been used in the treatment of inflammation, including Syzygium aromaticum (L.) and eugenol as one of the most active compounds in it. Inflammation is one of an important biological response to injury. Anti-inflammatory is important to treat the danger of acute and chronic inflammation. The objective of the present study was to evaluate anti-inflammatory potential of S. aromaticum flower bud (clove) ethanol extract and eugenol from S. aromaticum on LPS stimulated-murine macrophage cell line (RAW 264.7). Cell viability assay was performed to evaluate the nontoxic concentration in cell line was performed by MTS assay. The cytotoxic activity which is considered safe on RAW.264.7 cell were 50 and 10 μg mL–1 concentration of the S. aromaticum flower bud ethanol extract and eugenol. Several measurements were performed including IL-1β, TNF-α, NO and IL-6 concentration assay by Elisa after treatment compared to non treated cells to determine the anti-inflammatory activity. Syzygium aromaticum was inhibited IL-1β, TNF-α, NO and IL-6 release on LPS stimulated-RAW.264.7. No significant difference was observed in the S. aromaticum flower bud extract and eugenol on IL-1β inhibitory activity but eugenol showed the higher TNF-α inhibitory activity then S. aromaticum flower bud extract. The 50 μg mL–1 ethanol extract of S. aromaticum flower bud showed the highest IL-6 and nitrite-associated NO inhibitory activity. This research revealed that ethanol extract and eugenol of S. aromaticum flower bud (clove) possess the anti-inflammatory potential showed by the inhibitory activity of the inflammatory mediator including IL-1β, TNF-α, NO and IL-6.
  Betty Nurhayati , Marlia Singgih Wibowo , Yantyati Widyastuti , Pande Putu Erawijantari , Wahyu Widowati , Mohammad Rizki Fadhil Pratama and Tutus Gusdinar Kartawinata
  Lactobacillus plantarum species often harbor several plasmids. These plasmids may encoded important traits such as phages or antibiotics resistances, lactose catabolism and production of proteolytic enzyme and also bacteriocins that named plantaricin. Lactobacillus plantarum IBL-2 that isolated from strawberry of Bali plantation have the highest anti-microorganism activity among the L. plantarum isolate collection of BTCC Indonesia. Only few study carried out to examine the antifungal activity of bacteriocin from L. plantarum. This study focus on anti-Candida activity of plasmid associated with bacteriocin production from L. plantarum IBL-2. The isolates were confirmed by the 16S rRNA analysis by PCR and the phylogenetic tree was built based on references sequences and one outgroups from database. The plantaricin gene screening then performed in plasmid that was isolated from L. plantarum IBL-2 by PCR using five pairs or plantaricin gene primer. The anti-Candida potential of plantaricin observed were analyzed by in silico by docking analysis between the plantaricin and receptors of apoptosis proteins. PlnB and PlnEF were observed in the L. plantarum plasmid. Only PlnEF become the focus of study. Analysis of docking study predicted that PlnEF have interactions with apoptosis proteins regulator in eukaryotic cells. PlnEF that encoded by plasmid of L. plantarum may exert anti-Candida potential through interactions with apoptosis proteins regulator.
  Rostika Flora , Mohammad Zulkarnain , Esti Sorena , I Dewa Gde Sathya Deva and Wahyu Widowati
  During physical activity, oxygen reduction leading to hypoxia affects brain cell metabolisms, induces stabilization of Hypoxia Inducible Factor-1α (HIF-1α) and up-regulate Vesicular Endothelial Growth Factor (VEGF). This study aimed to investigate the correlation of HIF-1α and VEGF of brain tissue in male wistar rat after anaerobic exercise. Twenty four rats were divided into four groups: control, 1x, 3x and 7x in a week of anaerobic exercise. A rat treadmill was used at speed 35 m min–1, 20 min for every anaerobic exercise. The HIF-1α and VEGF level were measured by ELISA. The correlation between HIF-1α and VEGF was analyzed using Pearson test. The HIF-1α and VEGF level increased in 1x and 3x a week of anaerobic exercise. The highest HIF-1α and VEGF level were observed in 1x a week of anaerobic exercise. Pearson test between HIF-1α and VEGF showed r = 0.709 and p = 0.000. These findings showed that HIF-1α and VEGF are correlated and anaerobic exercise affects HIF-1α and VEGF level.
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