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Articles by Wafaa A. Abd El-Ghany
Total Records ( 5 ) for Wafaa A. Abd El-Ghany
  Wafaa A. Abd El-Ghany , M.H. Awaad , S.A. Nasef and A.F. Gaber
  This study was designed to examine the effect of using sodium butyrate encapsulated in palm fat in comparison with enrofloxacin on Salmonella Enteritidis (SE) infection in broiler chickens. Two hundred, day-old broiler chicks were allocated into 5 equal groups (n = 50). Group 1 was kept without challenge or treatment (blank control), Group 2 was fed on sodium butyrate, Group 3 was challenged and treated with enrofloxacin, Group 4 was challenged and treated with sodium butyrate and Group 5 was only challenged (positive control). Challenged groups were orally inoculated with 0.3 mL (1.5×108 SE/mL/chick) at the 2nd day of age. Enrofloxacin was given at the 3rd day of age in water (10 mg kg–1 b.wt.) for 5 successive days; however, sodium butyrate was added in doses of 1.0, 0.5 and 0.25 kg t–1 for starter, grower and finisher ration respectively from day till 5 weeks old. The results revealed no mortalities and decrease in the severity of signs and lesions in treated groups than positive control one. At the 4th week of age, sodium butyrate supplement gave significant (p≤0.05) improvement in body weight, weight gain and feed conversion than others. The re-isolation rate and enumeration of SE were lower in sodium butyrate and enrofloxacin treatments than positive control. In conclusion, sodium butyrate as an acidifier could be used as an environmentally friendly supplement when compared with enrofloxacin for treatment of SE infection in broiler chickens as it could reduce the disease picture severity, improve performance variables and decrease the intestinal colonization.
  M.H.H. Awaad , Wafaa A. Abd El-Ghany , S.A. Nasef , Medhat S. El-Halawany , Faten F. Mohamed and Ahmed F. Gaber
  Objectives: The efficacy of microencapsulated Na-butyrate (NaB) supplementation for reducing Salmonella enteritidis (S. enteritidis) infection was investigated. Methodology: One day old broiler chicks (n = 200) were allotted into 4 equal groups. At 2nd day of age, birds of groups 3 and 4 were challenged by crop gavage with 5x108 CFU mL–1 of S. enteritidis. Groups 2 and 3 were supplemented with NaB diet while 1 and 4 were fed on a plain ration without treatment. Scanning Electron Microscopy (SEM) of re-isolated S. enteritidis of challenged NaB treated broilers revealed broken cells with morphological changes in comparison with untreated challenged group, however, Transmission Electron Microscopy (TEM) showed several lipids like bodies that barely detected in untreated group. Results: Results from Polymerase Chain Reaction (PCR) analyses revealed that S. enteritidis strain from untreated challenged control harbored virulence genes invA, stn and pefA with incidence rate of 100%. While, NaB supplementation resulted in detection of 100% of invA gene and only 66.6% of pefA and stn genes. Gut integrity of NaB supplemented groups showed significant (p≤0.05) increase in villi height and villus height: crypt depth ratio and had positive effect in maintenance of healthy intestinal epithelial cells. Conclusion: NaB supplement is effective in reducing S. enteritidis in chickens.
  M.M. Zaki , Wafaa A. Abd El-Ghany , Maha M. Hady and Reda M.S. Korany
  Objective: In this study, the immunopotency of dried coriander seed (Cr), turmeric (Tu) and thyme (Th) powders feed additives in broilers was investigated. Methodology: Three hundred, day old broiler chicks were divided into 5 groups, each of 3 replicate and kept for a period of 35 days. A basal diet was used to which Cr, Tu and Th were added either solely at level of 0.75% or in mixture (Mx), while control fed basal diet (C). Haemagglutinating Inhibiting (HI) antibody titre against Newcastle Disease (ND) was measured in sera of all birds. By the end of the experimental period, three birds from each replicate were weighed and killed then bursa (Br), thymus (Thy) and spleen (Sp) were extracted to calculate weight indices and fixed in formol buffer for morphometric measurements. Results: The HI titres were higher (p<0.05) in Cr and Tu compared with others. The Br, Thy and Sp weight indices were higher (p<0.05) in all groups than C, while those of Tu, Th and Mx groups were higher (p<0.05) than Cr group. The morphometric measurements were higher (p<0.05) in all treated groups compared to C, while values of Tu were higher (p<0.05) than Th and Cr groups. Conclusion: Dried Cr and Tu feed additives had an immunomodulatory effect in broilers.
  Wafaa A. Abd El-Ghany
  In this investigation, a trial for preparation and evaluation of locally prepared (autogenous) bacterin against the infection with Avibacterium paragallinarum (Avi. paragallinarum) causing infectious coryza disease in layer chickens was done. Two types of adjuvant (aluminum hydroxide and mineral oil based) were compared. At 6 weeks of age, one hundred layer chickens were divided into equally distributed 4 groups each containing 25 birds. Group (1) was vaccinated with Avi. paragallinarum autogenous bacterin containing aluminum hydroxide, while group (2) received Avi. paragallinarum autogenous bacterin containing mineral oil. Both types of bacterins were given in a dose of 0.5 ml/bird and administered intramuscularly (IM). Booster dose of both types of the autogenous bacterins was given in group 1 and 2 at 9 weeks of age. Chickens of group (3) were kept without vaccination. Birds in groups 1, 2 and 3 were challenged with (106 CFU) of live Avi. paragallinarum culture by inoculation into the nasal sinus at 12 weeks old. Group (4) was left as blank control negative (not vaccinated or challenged). Birds after challenge were kept under complete daily observation for 7 days. Signs, mortalities, postmortem lesions, protection rate and reisolation rate of the organism were taken as criteria for bacterin evaluation, also agglutination test were performed on sera to determine the immune response to bacterin at the doses intervals. The results revealed that whatever the type of adjuvant, both different adjuvanted types' bacterines were effective and safe in prevention of infection against Avi. paragallinarum in layers when administered at 6 and boosted at 9 weeks of age.
  Wafaa A. Abd El-Ghany and M. Ismail
  This study was designed to compare between the efficacy of a phytobiotic containing a mixture of essential oils of Oreganum aetheroleum and an antibiotic containing ciprofloxacin as an active principle for the treatment of experimental (E. coli) infection in broiler chickens. Two hundred, day old broiler chickens were divided into 5 equal groups. The 1st group was neither challenged nor treated but groups 2, 3, 4 and 5 were challenged with E. coli. The 2nd group was challenged only while the 3rd, 4th and 5th group were treated with phytobiotic, ciprofloxacin and phytobiotic and ciprofloxacin combination, respectively. Results confirmed significant (p<0.05) improvement of productive performance parameters, reduction in signs, mortalities, post mortem lesions and bacterial re-isolation, significant (p<0.05) enhancement in cell mediated and humoral immune responses, significant (p<0.05) reduction in levels of liver and kidney function tests as well as significant (p<0.05) increase in the total protein and globulin levels in chickens challenged with E. coli and treated with either essential oils or ciprofloxacin compared with challenged non treated chickens. Moreover, the best significant (p<0.05) results in all measured parameters were detected in group treated with combination in comparison with those treated with single treatment. In conclusion, a mixture of essential oils of Oreganum aetheroleum is effective for the treatment of E. coli infection in broiler chickens when compared with ciprofloxacin. However, combined treatment could be superior in controlling such infection of broiler chickens.
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