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Articles by W.J. Liu
Total Records ( 6 ) for W.J. Liu
  Y. Fang , W.J. Liu , F.Q. Zhang , Y.G. Shao and S.G. Yu
  Genetic variations of KAP13.1 gene have been studied in 816 animals of Xinjiang goat breeds in China. The genotypes and allele frequencies of KAP13.1 gene were detected by PCR-RFLP techniquies. At the same time, parts of our samples were sequenced and analyzed with cashmere production traits data. The results showed that TT genotype significantly higher of body weight after combed trait than GT genotype (p<0.05) and down cashmere thickness trait showed genotype GT with significant higher than GG (p<0.05) in Nanjiang cashmere goat. In Xinjiang goat, the fiber diameter showed significant differences (p<0.05) among the age two, three and four years old. The fiber diameter trait in one year old cashmere goat had significant differences (p<0.05) compared with two, three and four years old. The frequencies of the KAP13.1-T allele in Xinjiang goat (n = 220), Nanjiang cashmere goat (n = 310) and Bogeda cashmere goat breeds (n = 286) were 0.996, 0.568 and 0.969, respectively. The χ2-test showed that the genotype distributions in these three cashmere goat breeds were not in agreement with Hardy-Weinberg equilibrium. According to the classification of PIC, Nanjiang cashmere goat was more polymorphic at this locus. The mutation in our animals at KAP13.1 locus was recorded for the first time. In conclusion, The results possibly revealed that the polymorphism existed in the three Xinjiang local goat breed and a missense mutation was possibly caused by variations in the number of the decapeptide repeat structures. Further analysis of results leads us to believe that the polymorphism of KAP13.1 gene might be relevant to fiber diameter and other cashmere traits. Thus, molecular genetic study of KAP13.1 gene represented valuable results for genetic conservation purposes and economic production of cashmere traits.
  X.K. Yao , S.J. Yu , W.J. Liu , J. Meng , X.D. Pan , X.H. Tan and Q.J. Luo
  This study was designed to understand the dynamic changes of physical and chemical indicators in the Yili horse colostrum in order to provide the scientific basis for Yili horse breeding. Fat, non-fat solids, protein, density, freezing point, ash, electrical conductivity and so on in milk between Yili horse and F1 hybridization mare of new Kirghiz horse and Yili horse (hybrid horse for short) in 2, 4, 6, 8, 12, 24 and 48 h, 72 and 96 day, 120, 144 and 168 h after foaling were compared and analyzed. The results showed that: (1) Protein, non-fat solids, density, ash, electrical conductivity in colostrum of Yili horse and hybrid horse were higher than that in milk and gradually close to milk with the extension of lactation. (2)Protein, ash and non-fat solids in colostrum of Yili horses and hybrid horse were decreased dramatically in 12 h after foaling. (3) Protein, density, ash, electrical conductivity and non-fat solids in Yili horse colostrum were different with hybrid horse at the same lactation period, as most measured values of Yili horse were higher than hybrid horse and the difference was gradually narrowing with extended lactation period. (4) Protein in colostrum of Yili horse after foaling in 4 h were extremely significantly higher than that in hybrid horse (p<0.01).(5) Protein, density and crude ash in colostrum of Yili horse after foaling in 72 h were significantly higher than that in hybrid horse (p<0.05). (6) Non-fat solids in colostrum of Yili horse after foaling in 8 h were significantly higher than that in hybrid horse (p<0.05).
  J.T. Yang , R.X. Zang , W.J. Liu , H.W. Xu , J.L. Bai , J.X. Lu and J.P. Wu
  Diacylglycerol acyltransferase 1 (DGAT1) is considered the key enzyme in controlling the rate of synthesis of triglycerides. The DGAT1 gene was recently identified as a strong functional candidate gene affecting mutton quality traits (e.g., intramuscular fat) in sheep. The objective of present study was to inspect the single nucleotide polymorphism (SNP) of exon 16-17 of DGAT1 gene in four Chinese indigenous sheep breeds. A total of 167 sheep (Tan sheep (TS), n = 58, Ganjia sheep (GS), n = 36, Oula sheep (OS), n = 39 and Qiaoke Sheep (QS), n = 34) were used for investigating. The SNP was detected by Restriction Fragment Length Polymorphism (RFLP) and further confirmed by sequencing PCR products. A SNP (C→T) was identified in 4 Chinese sheep breeds, which creates a non-synonymous single base mutation (GCC(Ala)→GCT(Ala)) at restriction site for endonuclease Alu I (AGCT). The results of PCR-RFLP analysis showed that the SNP had three genotypes of TT (272 and 37 bp), TC (309, 272 and 37 bp) and CC (309 bp), in which CC was the predominant genotype and allelc C was predominant allele in 4 sheep breeds. The genotype of SNP in TS and OS was in agreement with Hardy-Weinberg equilibrium (p>0.05), in turn GS and QS was not in agreement with Hardy-Weinberg equilibrium (0.01<p<0.05). The allelic diversity was 0.29, 0.33, 0.35 and 0.36 and heterozygosity was 0.35, 0.42, 0.45 and 0.47, respectively in the TS, GS, OS and QS. The above mentioned results indicated that these 4 Chinese indigenous sheep breeds are moderate polymorphism.
  W.J. Liu , Y. Fang , G.X. Fang , M. Wang , H. Yu , X.L. Li , T.T. Feng , H. Chen and S. G. Yu
  In the present study, the keratins and Keratin-Associated Proteins (KAPs) are one of the largest gene families in mammalian genomes encode, which is a heterogeneous group of proteins that make up about 90% of the cashmere fiber. Also, it regarded as a candidate gene of cashmere production traits. In this study, we aimed to detect polymorphisms of KAP16.6 gene and to investigated their associations with cashmere production traits (fiber diameter, cashmere yield, down cashmere thickness, body weight after combed cashmere) of three local goat breeds in China. In Xinjiang goat, statistical evaluation revealed significant differences (p<0.05) between the fiber diameter and cashmere yield trait of GG genotype. In Nanjiang cashmere goat, it is no significant differences (p>0.05) between cashmere production traits. The missense mutation of KAP16.6 gene in 816 cashmere goat samples was firstly detected in three of Xinjiang local goat breeds. Also, parts of these samples were sequenced. The results showed that frequencies of the KAP16.6-G allele in Xinjiang goat (n = 220), Nanjiang cashmere goat (n = 310) and BoGeDa cashmere goat breeds (n = 286) were 0.705, 0.603 and 0.600, respectively. The χ2 test showed that the genotype distributions in these three cashmere goat breeds were in agreement with Hardy-Weinberg equilibrium. According to the classification of PIC, BoGeDa cashmere goat was more polymorphic at this locus. Then a missense mutation was described at KAP16.6 locus in Xinjiang local goat breeds for the first time. The results possibly revealed that the size polymorphism existed in the three Xinjiang local goat breeds.
  W.J. Liu , Y. Fang and L.J. Li
  The wool fiber is structurally composed of low-sulfur proteins, for which the Keratin Intermediate-Filaments (KIF) gene is responsible along with Keratin-Associated Proteins (KAPs). In this study, PCR-RFLP and DNA sequencing were used to detect polymorphisms of the Keratin Intermediate-Filaments type I (KIFI) gene in three Chinese cashmere goat breeds (Xinjiang, Nanjiang and Bogeda White), to estimate gene and genotype frequencies and Polymorphism Information Content (PIC) and to determine impacts of genotype for KIFI on expression of cashmere traits. Results showed a novel A-C (GGCA-to-GGCC) mutation in intron 1, which forms a HaeIII endonuclease restriction site. Three unique PCR-RFLP banding patterns (genotypes AA, AC and CC) were found. The frequencies of the A allele in the samples from the goat breeds varied from 0.700-0.747. The genotypic distributions in three cashmere goat breeds were in Hardy-Weinberg equilibrium (p>0.05). According to the classification by PIC, the Xinjiang cashmere goat breed was more polymorphic at this locus than the other breeds. Furthermore, analysis of the impact of KIFI gene polymorphism on cashmere traits (cashmere fineness, down cashmere thickness, cashmere yield, body weight after combing) in goats from the Xinjiang breed indicated greater cashmere fineness in genotype AA compared to genotype CC (p<0.05), suggesting that this mutation may have significant influence on the cashmere fineness.
  X. Yu , X.X. Huang , W.J. Liu , Y. Fang , L. Shi , W.T. Xing , C.W. Tang and M.A. Brown
  The genetic variability and genetic relationship of the fifteen sheep breeds were studied. The genotypes of 14 indigenous Chinese sheep breeds and one crossbreed of wild sheep were investigated using 13 microsatellite DNA markers recommended by the Food and Agriculture Organization of the United Nations (FAO) and the International Society of Animal Genetics (ISAG) through PCR. The allele frequency, heterozygosity and Genetic Differentiation Index (Fst) were computed to estimate the genetic variation of each population. To determine the genetic relationships among the breeds, phylogenetic trees were constructed based on Nei’s genetic distance using the unweighted pair-group method with arithmetic mean and neighbor-joining method. A total of 15 breeds were clustered into three groups. The first group included Altay sheep and Duolang sheep, the second group was very large and complicated, the third group was a Kazark sheep. The thirteen microsatellite loci were effective markers for the analysis of genetic relationship among sheep breeds. In addition, genetic distance among groups is not according with their geographic distribution and groups or breeds with low production performance can easily impacted by breeds with better production performance. As for the results, it may be result from applying on artificial insemination and extension of commercial cross breeding technique in sheep production system recent years in China.
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