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Articles by W.E. Wan Khadijah
Total Records ( 4 ) for W.E. Wan Khadijah
  A.A. Anna , M.N. Hisham , W.E. Wan Khadijah and R.B. Abdullah
  A strategize step in oocyte recovery is important and beneficial in producing successful production of high-quality Cumulus Oocyte-Complexes (COC) for in vitro maturation, fertilization and culture (IVMFC), nuclear transfer and other emerging technologies. Experiments were conducted to compare Oocyte Recovery (OR) between Laparoscopic Ovum Pick-Up (LOPU) and ovarian slicing and to evaluate the effects of OR cycle, hormone stimulation, oestrus synchronization, breeds, liveweight and age of goats on the quantity and quality of oocytes obtained. In Experiment 1, oocytes were recovered from abattoir-derived ovaries by the slicing method. The ovaries were transported to the laboratory for immediate harvesting. The slicing method yielded significantly (p<0.05) larger number of oocytes per ovary than LOPU (22.53±2.78 vs. 6.10±0.46). However, the proportion of Grade A oocytes was highest from the LOPU technique (30.51±4.14) compared to the slicing method (19.07±3.57). LOPU resulted in good quality Cumulus Oocytes Complexes (COC) with more than 5 layers of cumulus cells. Experiment 2 consisted of three OR cycles (OR-1, OR-2 or OR-3); four durations of oestrus synchronization using CIDR (Day-10, -14 , -17 or -21); three goat breeds (Boer Crossbred, Mixed breed or Katjang); 4 age groups (Young, Mature, Old or Very Old) and 4 levels of body weight (20, 21-29, 30-37 or 38 kg). Goats were synchronized using Control Internal Drug Release (CIDR) combined with 125 μg Estrumate and hyperstimulated with 70 mg FSH and 400 IU hCG after the removal of CIDR 24 h later. There was no significant effect of OR cycle, CIDR removal goat breed, age or body weight on the total number of oocytes recovered per ovary. Katjang goats yielded a higher proportion of Grade A oocytes compared to Boer Crossbreds and mixed breeds which had a higher proportion of Grade C oocytes (p<0.05). Age and weight of animals had no significant effect on the quality of oocytes recovered. Grade A and B oocytes were obtained in OR-1, whilst the repetition of LOPU resulted in an increment of Grade C oocytes in OR-2 and OR-3, respectively. Day-10 and -21 gave the highest cumulative percentage of 58 and 64% for Grade A and B oocytes, respectively. Under the conditions of the experiment, LOPU yielded better quality oocytes from the Katjang goats using less or no OR repetition, with oestrus synchronization at 10 or 21-Day.
  M.M. Rahman , M. Ikeue , M. Niimi , R.B. Abdullah , W.E. Wan Khadijah , K. Fukuyama and O. Kawamura
  A survey of oxalate and its related mineral contents in selected fodder plants was conducted in two regions of subtropical Okinawa, Japan and of tropical Savar and Shahzadpur, Bangladesh. A total of 31 samples were taken from 13 fodder species in Okinawa, Southern part of Japan and of 63 samples from 27 fodder species in Bangladesh. The data of both regions revealed that the majority of fodder plants accumulated lower contents of oxalate than the critical level for toxicity at more than 20 g kg-1 DM, while few fodder species (Pennisetum purpureum and Brachiaria mutica) in Bangladesh and only Setaria sphacelata in southern part of Japan reached this critical level. In most of the cases, no relationship was found between oxalate and mineral contents in the plants tested. The results from the present study demonstrate that the oxalate content in tropical fodder species may vary in a wide range, mainly depending on plant species. To be noted is that some fodder species could accumulate oxalate at so high content as might be toxic to ruminants in certain conditions.
  A.B. Ainul Bahiyah , W.E. Wan Khadijah and R.B. Abdullah
  This study evaluated the efficiency of direct plunging and vitrification techniques in preserving mouse embryos at various preimplantation stages. Embryos were from superovulated ICR female mice. In direct plunging, embryos were equilibrated in EG (4.0 M), sucrose (0.25 M), BSA (4%), loaded into straw and plunged into LN2. The straw was thawed in water bath (37°C) and embryos were equilibrated in sucrose (0.5 M), BSA (4%). In vitrification, embryos were equilibrated with EG (7.5%), DMSO (7.5%) with EG (15%), DMSO (15%) and loaded onto cryoleaf. The cryoleaf was plunged into LN2. Embryos were warmed in sucrose (1.0 M), sucrose (0.5 M), washing solution and in modified WM medium. Frozen-thawed/vitrified-warmed embryos were cultured in modified WM medium in 5% CO2 incubator (37°C). Some embryos were transferred into pseudopregnant CBA/ca females for in vivo viability assessment. Vitrification gave higher survival rates than direct plunging technique at all developmental stages studied. No significant differences in the percentage of live-birth from direct plunging (22.40±4.40%), vitrification (29.80±9.00%) and control embryos (28.83±3.25%).
  M.M. Rahman , M. Tateyama , M. Niimi , R.B. Abdullah , W.E. Wan Khadijah and O. Kawamura
  Oxalate concentration in forage plants is important, because it results mineral deficiency in ruminants. Data on oxalate concentration in forage plants in conjunction with cutting and uncutting conditions throughout the growing period are limited. This study was aimed to investigate the changes in oxalate and some mineral concentrations of setaria (Setaria sphacelata). The plants were harvested at different stages (vegetative, boot, pre-flowering, flowering and seed) of maturity and at about 50 cm in length of regrowth (second to sixth cuttings) for evaluation of soluble oxalate, insoluble oxalate and some mineral concentrations. Soluble oxalate and total oxalate concentrations, as well as mineral concentrations, decreased with advancing maturity. Both oxalate concentrations (soluble or insoluble) were higher in leaf compared to stem. Soluble oxalate and total oxalate concentrations of regrowth were the highest at third cutting and lowest at sixth cutting. Insoluble oxalate concentration of regrowth was almost similar in all cuttings, except for the sixth cutting. The highest concentrations of potassium, sodium and magnesium of regrowth were observed at third cutting, while the highest concentration of calcium was observed at sixth cutting. A relationship between oxalate and mineral concentrations was partially observed. Results suggest that cutting materials of setaria from June to October could achieve oxalate levels that are toxic to ruminants.
 
 
 
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