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Articles by W.D. Ratnasooriya
Total Records ( 2 ) for W.D. Ratnasooriya
  J.R.A.C. Jayakody , W.D. Ratnasooriya , W.A.N.A. Fernando and K.R. Weerasekera
  Ruta graveolens Linn (Family: Rutaceae) leaves are used in Sri Lankan traditional medicine as a diuretic but this effect is not scientifically validated. This study has evaluated the diuretic potential of R. graveolens leaves in rats using a Hot Water Infusion (HWI). Different concentrations of HWI (4.5, 6.75, 9.0 mg mL-1) or vehicle or furosemide (13 mg kg-1) were orally administrated (n = 6 per group) to hydrated rats and their urine output was monitored hourly for 6 h. Urinary pH, specific conductivity, specific gravity, Na+, K+, Cl¯ levels and creatinine clearance were determined (with the highest dose and control). Using these data standard urinary indices were calculated. Further, subchronic toxicity was examined in terms of serum Glutamic Oxaloacetic Transaminase (GOT), Glutamic Pyruvic Transaminase (GPT), urea and creatinine levels and overt signs. HWI increased the urine output markedly in a dose-dependent manner. The high dose of HWI was almost equipotent to furosemide (in terms of diuretic activity). The onset of diuresis was very rapid (within 1 h) and lasted throughout the studied period. HWI also caused significant increase in specific gravity, specific conductivity, creatinine clearance, Na+ and K+ levels, thiazide secretion index, urine alkaline index, diuretic action, Na+ and K+ saliuretic indices and significant decrease in carbonic anhydrase index. Further, no evidence of subchronic toxicity was seen. R.graveleons leaves exhibits safe and strong oral diuretic activity as claimed in Sri Lanka traditional medicine. This action is mediated via multiple mechanisms: thiazide like activity, inhibition of carbonic anhydrase activity and increase in glomerular filtration rate.
  R.M. Dharmadasa , G.A.S. Premakumara , P.L. Hettiarachchi and W.D. Ratnasooriya
  Munronia pinnata (Wall.) Theob. (Meliaceae) is therapeutically important medicinal plant used for the control of malaria in traditional medicine in Sri Lanka. This study described the cytotoxic potential of five morphotypes and antimalarial activity of the highest cytotoxic morphotype of M. pinnata using brine shrimp toxicity assay and Plasmodium yoelii murin model, respectively. Four concentrations of (0.1, 1, 5 and 10 mg mL-1) aqueous extracts of stem, roots and leaves of five morphotypes of M. pinnata were screened for cytotoxcity using brine shrimp assay. Either, three doses (3200, 1600, 800 mg kg-1) of the whole plant aqueous extract of the highest cytotoxic morphotype of M. pinnata (WPAE) or vehicle or quinine were orally administered for different groups of mice (n = 6) inoculated with P. yoelii murine model. Antimalarial activity was examined in terms of schizonticidal activity and chemo suppression. Overt signs of toxicity, body weight, rectal temperature, stress and aversive behavior were observed with long term oral administration of high dose of WPAE (n = 10). All parts of five morphotypes of M. pinnata showed potent cytotoxic activity. The order of potency was roots>stems>leaves. The highest cytotoxicity exhibited by morphotype collected from Nilgala forest reserve (MGNG3). The high dose of WPAE of MGNG3 has potent oral antimalarial activity. No signs of overt toxicity, signs of stress and aversive behaviour observed during long term toxicological studies. This study showed, for the first time, that all morphotypes of M. pinnata has marked cytotoxcity and morphotype MGNG3 has potent antimalarial activity as claimed by Sri Lankan traditional practitioners.
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