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Articles by W.D. JIANG
Total Records ( 3 ) for W.D. JIANG
  L. FENG , H.H. HUANG , Y. LIU , J. JIANG , W.D. JIANG , K. HU , S.H. LI and X.Q. ZHOU
  This study was to investigate the effect of dietary thiamin on the immune response and intestinal microflora in juvenile Jian carp (Cyprinus carpio var. Jian). Seven diets (0.25, 0.48, 0.79, 1.06, 1.37, 1.63 and 2.65 mg thiamin kg−1) were fed to Jian carp (8.20 ± 0.02 g). At the end of feeding trial, red blood cell counts and white blood cell counts increased with increasing dietary thiamin levels up to 0.79 and 1.06 mg kg−1 diet, respectively (< 0.05); Lactobacillus counts in intestine increased gradually, and maximum values obtained when the thiamin level was at 0.79 mg kg−1 diet (< 0.05). After the feeding trial, fish were injected with Aeromonas hydrophila only one time on the first day of the challenge trial and fed the same diets as the growth trial for 17 days. The survival rate, leucocyte phagocytic activity, lectin potency, acid phosphatase activity, lysozyme activity, total iron-binding capacity and immunoglobulin M content of fish after being injected with A. hydrophila were all improved with increasing dietary thiamin levels up to a point, respectively (< 0.05). These results suggested that thiamin could improve immune response and the balance of intestinal microflora of juvenile Jian carp.
  L. FENG , W.W. XIAO , Y. LIU , J. JIANG , K. HU , W.D. JIANG , S.H. LI and X.Q. ZHOU
  Oxidative damage and antioxidant status of intestine and hepatopancreas for juvenile Jian carp (Cyprinus carpio var. Jian) fed graded levels of methionine hydroxy analogue (MHA: 0, 5.1, 7.6, 10.2, 12.7, 15.3 g kg−1 diet) for 60 days were studied. Radical scavenging ability, antioxidant enzymes activities such as superoxide dismutase (SOD), catalase (CAT), glutathione-S-transferase (GST), glutathione peroxidase (GPX) and glutathione reducase (GR), as well as glutathione (GSH), protein carbonyl (PC) and malondialdehyde (MDA) contents were assayed in these tissues. Results indicated that anti-superoxide anion capacity in intestine and anti-hydroxyl radical capacity in hepatopancreas significantly improved with dietary MHA levels up to 7.6 and 10.2 g kg−1 diet respectively, whereupon they decreased (< 0.05). SOD, CAT, GST, GPX, GR activities in intestine and hepatopancreas, as well as GSH content in hepatopancreas significantly increased with optimal MHA levels which were in the range of 5.1−10.2 g kg−1 diet, and thereafter decreased (< 0.05). Meanwhile, MDA and PC contents in these tissues together with GOT and GPT activities in plasma significantly decreased with optimal MHA levels which were in the range of 5.1−7.6 g kg−1 diet, and thereafter increased (< 0.05). These results suggested that MHA improved antioxidant status and depressed lipid and protein oxidation in intestine and hepatopancreas.
  N.B. XIE , L. FENG , Y. LIU , J. JIANG , W.D. JIANG , K. HU , S.H. LI and X.Q. ZHOU
  A 9-week feeding trial was carried out with juvenile Jian carp (Cyprinus carpio var. Jian) to study the effects of dietary phosphorus on growth, body composition, intestinal enzyme activities and microflora. Quadruple groups of juvenile Jian carp (7.17 ± 0.01 g) were fed practical diets containing available phosphorus 1.7 (unsupplemented control), 3.6, 5.5, 7.3, 9.2 and 11.0 g kg−1 diet to satiation. Feed intake, specific growth ratio and feed efficiency were the lowest in fish fed the basal diet (< 0.05). Body moisture, protein, lipid content and ash were all significantly affected by dietary available phosphorus levels (< 0.05). Activities of trypsin, amylase, Na+, K+-ATPase, alkaline phosphatase and gamma-glutamyl transpeptidase were improved with increasing dietary phosphorus levels. Intestinal Aeromonas and Escherichia coli decreased with increasing dietary phosphorus up to 3.6 and 5.5 g kg−1 diet respectively (< 0.05), while Lactobacillus increased with the increasing dietary phosphorus up to 9.2 g kg−1 diet (< 0.05). These results suggested that phosphorus could enhance intestinal enzyme activities of juvenile Jian carp and the minimum dietary available phosphorus requirement for SGR of juvenile Jian carp (7.2−63.8 g) was 5.2 g kg−1 diet.
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