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Articles by W.B. Bao
Total Records ( 5 ) for W.B. Bao
  G.H. Chen , J.H. Cheng , E.S. Shuiep , W.B. Bao and H. H. Musa
  Total of 120 chickens from Anka and Rugao breed includes (60 individual per breed and 30 individual per sex) were taken as a representative sample and were slaughtered at Jiangsu Poultry Institute, China, and then the carcasses were dissected manually. Water holding capacity, color density, pH and tenderness were estimated from breast muscle. Breeds were found differed significantly (P< 0.05) in color density, pH and tenderness. Regarding to water holding capacity no significant (P>0.05) different were noted between breeds. Tenderness, the values for shear force were significantly (P>0.01) higher in males than females in two chicken breeds. In addition color density, pH and water holding capacity were non significantly difference (P>0.05). In Anka chicken breed color density was positive correlated with pH, tenderness and water holding capacity, and similarly pH was positively related with tenderness. While in Rugao all meat quality traits shows negative correlation with each others, specially tenderness was observed positive correlation with color density.
  H.H. Musa , B.C. Li , G.H. Chen , T.P. Lanyasunya , Q. Xu and W.B. Bao
  Traditional karyotyping is invented in animal research for several decades depend on the analysis of characteristic banding patterns along the length of chromosome. In the present study chicken metaphase chromosomes were obtained by peripheral blood lymphocyte culture techniques, G-band patterns were obtained with trypsin and Giemsa, C-band patterns were treated with barium and the nuclear organizer regions (NORs) were identified by silver staining. All species studied presented a diploid number of 78 chromosomes, with 10 pairs of macro chromosomes including the sex chromosome and 29 pairs of micro chromosomes. G-band patterns were found quite different between breeds. The dark stained of C-band was observed on micro chromosome and W chromosome. Karyotype resemblance near coefficient was possible for breeds clustering. The position of centromers, relative length, arm ratio and the evolutionary distance of chicken breeds was estimated. The application of chromosome karyotype and banding techniques was used to study the origin, evolution and relationship of species, also used for gene location and sex determination. While, in the Medical field was used to identify genetic disease. The techniques was consider as a base for further molecular research, for example FISH.
  W.B. Bao , J.T. Shu , H.H. Musa and G.H. Chen
  Genetic distance and gene flow of all pairwises of 14 Chinese indigenous chicken breeds and one jungle fowl and their relations with geographical distances were evaluated in the present study. The pairwise FST/(1-FST) of 15 Chinese chicken breeds was evaluated by 29 microsatellite loci. The results showed that the number of alleles per locus was ranged from 2-25 and the average of expected heterozygosity and PIC of all loci were 0.6683 and 0.50, respectively. The average of genetic differentiation among population measured as FST value, was 16.4% (p< 0.001), all loci were contributed significantly (p< 0.001) to this differentiation. Red jungle fowl and Gushi chickens were observed distant genetic relationship with other breeds, whereas Huainan Partridge and Tibetan chickens were observed close relationship with other breeds. The geographical elements may own the close relationship for particular population pairs. However, the equation FST/ (1-FST) = -0.0162 + 0.0313ln (d) and the result of Mantelís test (p = 0.054) did not provide enough support for a significant correlation between the genetic and geographical pairwise distances.
  H.H. Musa , J.H. Cheng , W.B. Bao , J.T. Shu , B.C. Li and G.H. Chen
  Tissues specimens include (liver, kidney, small intestine and abdominal fat) were excised from lean (Rugao) and fat (Anka) chickens, both breeds were raised under the same conditions. The expression of LDL receptor mRNA in various tissues of genetically fat and lean chickens were determined by semi-quantitative RT-PCR. The level of gene expression was determined as the ratio of integrated peak area for each individual gene PCR product relative to that of the co-amplified ß-actin internal standard. The results shows that the expression of low density lipoprotein receptor mRNA was significantly (p< 0.05) different between abdominal fat and liver tissues, while they were non significantly different between the other tissues. The levels of LDLR1 mRNA expression in intestine, kidney and abdominal fat tissues were none significantly different between genetically fat and lean, while it was significantly (p< 0.05) different in liver. In addition, the levels of LDLR5 mRNA expression in various tissues were non significantly different between genetically fat and lean chickens.
  Musa H.H. , G.H. Chen , W.B. Bao , X.S. Wu and J.H. Cheng
  Meat quality such as tenderness, color Density (OD), pH and Water Holding Capacity (WHC) were estimated from breast muscle of genetically fat and lean chickens at 12 week of age. Mutation in lipoprotein lipase and apoVLDL-II genes was detected by PCR-SSCP techniques. Agreement of the genotype frequencies with the Hardy-Weinberg equilibrium expectations was tested using a chi-square goodness of -fit test. Lipoprotein lipase gene frequency was significantly different (p<0.01) in Rugao population, whereas in apoVLDL-II gene there are no significantly different between populations. The populations were differed significantly (p<0.01) within two genes. Lipoprotein lipase genotype was significantly (p<0.05) effect water holding capacity and meat tenderness. However, apoVLDL-II genotype was non significantly affected meat quality traits. The results also indicated that the interaction of Lipoprotein lipase and apoVLDL-II genotype was significantly (p<0.01) effected color density, pH and meat tenderness, whereas it was non significantly effects water holding capacity of breast muscle.
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