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by
W.A. Khalil |
Total Records (
2 ) for
W.A. Khalil |
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W.A. Khalil
,
Sh. A. Gabr
,
Sh. M. Shamiah
,
A.M.A. El-Haif
and
A.E. Abdel-Khalek
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This study aimed to compare the efficiency of three cryodevices (Conventional
Straws (CS), Hemi-Straws (HS) and spatula (SP)) in vitrification of buffalo
oocytes. Oocytes were recovered from ovaries of slaughtered buffaloes. They
were vitrified and thawed according to each cryodevice used. Then they were
in vitro matured, fertilized and cultured to blastocyst stage. Survival
and quality, in vitro maturation, fertilization rate and blastocyst production
rates of vitrified immature buffalo oocytes were assessed. Results showed that
total survival rate of the vitrified oocytes was higher (p<0.05) for SP and
HS (96 and 95%) than CS (80%). Recovery rate of normal oocytes relative to vitrified
or survival oocytes was the highest (p<0.05) for SP compared to other cryodevices.
In vitro maturation rate (oocytes at metaphase II stage) was lower (p<0.05)
for all vitrified oocytes than fresh (61.1 vs. 27.8-44%). SP showed higher (p<0.05)
maturation rate (44%) than HS and CS (34.4 and 27.8%, respectively). Cleavage
rate was lower (p<0.05) for all vitrified oocytes than fresh oocytes (37.7-58.9%
vs. 70.8), being higher (p<0.05) for SP and HS (55.8 and 58.9%, respectively)
than for CS (37.7%). Blastocyst production rate relative to cleaved oocytes
was lower (p<0.05) for vitrified than fresh oocytes (5.9-14.3% vs. 31.3)
but the differences among vitrification cryodevice were not significant. In
conclusion, immature buffalo oocytes can vitrified successfully by spatula,
conventional straws and Hemi-straws. Spatula device is a reliable alternative,
inexpensive and easy to assemble for vitrification of immature buffalo oocytes.
Also, vitrification spatula has the largest effective holding capacity. |
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M.A. El-Harairy
,
I.M. Abd El-Razek
,
E.A. Abdel-Khalek
,
S.M. Shamiah
,
H.K. Zaghloul
and
W.A. Khalil
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This study was conducted to determine the effect of different type and levels of antioxidant supplementation (0.4 and 0.8 mM from glutathione, GSH or 0.5 and 1.0 g L–1 extender from ascorbic acid, AA) as compared to control, on characteristics of camel epididymal spermatozoa stored at 25°C (room temperature) for 0, 2, 4 and 12 h or at 5°C (cool temperature) for 0, 12, 24 and 48 h. Testis of camel were collected after animal slaughtering and placed immediately into plastic bag into ice box at 5°C. Epididymal spermatozoa were collected by aspiration from tail and extended with tris-egg yolk extender. Results of epididymal spermatozoa stored at 25°C showed improvement in livability (p<0.05) and abnormality (p≥0.05) with GSH (0.4 mM), while sperm motility and curling spermatozoa improved (p≥0.05) with AA (0.5 g L–1). Storage at 5°C improved (p<0.05) motility, livability and curling spermatozoa with GSH (0.4 mM), while sperm abnormality improved (p<0.05) with AA (1 g L–1). At different incubation times at 25 or 5°C, percentages of motility, livability and curling spermatozoa decreased (p<0.05) and of sperm abnormality increased (p<0.05) by increasing storage time. The effect of interaction between antioxidant supplementation and storage time on all sperm characteristics studied was not significant. In conclusion, supplementation of tris-egg yolk extender with GSH (0.4 mM) or AA (0.5 g L–1) has a vital role in maintaining function, morphology and membrane integrity of epididymal camel spermatozoa stored at 25°C for 12 h or at 5°C for 48 h, respectively. |
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